Short communication: preliminary observations on the serum levels of HSP70 and its correlation with serum cortisol, thyroid hormones, and acute-phase protein concentration in cattle naturally infected with foot-and-mouth disease virus

Abstract

The study aimed to explore the serum levels of HSP70 and identify its possible association with serum cortisol, thyroid hormones, and acute-phase protein concentrations in cattle naturally infected with foot-and-mouth disease (FMD) virus. After the FMD outbreak in an organized dairy cattle farm in India, blood samples were obtained from clinically infected (n = 40) and apparently healthy (n = 30) animals. Samples were processed and tested by an in-house DIVA assay for confirmation of FMD infection. Serum was analyzed for HSP70, cortisol, T4, T3, haptoglobin, and serum amyloid A by enzyme-linked immunosorbent assay (ELISA). HSP70 concentrations were significantly higher in the serum of clinically infected cattle (p < 0.01) than the healthy group. To the best of our knowledge, this is the first report describing the elevated serum levels of HSP70 under infectious diseases of bovines. Cortisol (p < 0.05), haptoglobin (p < 0.001), and serum amyloid A (p < 0.05) concentrations also markedly increased in the diseased animals; however, no differences (p > 0.05) were found in T4 and T3 levels between healthy and infected cattle. Elevated HSP70 concentration correlated positively with high cortisol (p < 0.05) and haptoglobin (p < 0.001) levels suggesting an essential link between these acute events during clinical infectious phase of FMD. KeywordsSerum HSP70 • Cortisol • Thyroid hormone • Acute-phase proteins • Foot-and-mouth disease * Smrutirekha Mallick

Hematological Normality, Serum Biochemistry, and Acute Phase Proteins in Healthy Beef Calves in the Brazilian Savannah

“…The mean values observed are similar to those obtained by [122] in adult and young Holstein cattle, [123] in young taurine cattle in the finishing phase, and [124] in adult Zebu and Holstein crossbreeds. This suggests that there is no age or breed influence on the baseline value of the serum Hp concentration but rather the influence of the occurrence of diseases of an infectious character [125].…”

Thermoregulation responses in sheep: a cellular approach

“…Thus, heat stress has already been proven to increase plasma concentrations of amyloid A (Ríus et al, 2022). Another factor that may explain the higher expression of Serum amyloid A is the higher expression of HSP70 in this group (Ather et al, 2013;Mallick et al, 2021). Responses to extracellular HSPs appear to involve a variety of cell surface receptors that may determine their influence on tissue immune reactions (Calderwood et al, 2016).…”