Short communication: Relationship between the level of bovine leukemia virus antibody and provirus in blood and milk of cows from a naturally infected herd

Jaworski, Juan Pablo; Porta, Natalia Gabriela; Gutiérrez, Gerónimo; Politzki, Romina; Álvarez, Irene; Galarza, R.; Abdala, Alejandro Ariel; Calvinho, Luis Fernando; Trono, K.

doi:10.3168/jds.2015-10813

Cited by 20 publications

(29 citation statements)

References 24 publications

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“…During this period, calves are also fed with bulk-tank milk and balanced feed and then they are moved to pastures occupied exclusively by cattle of similar age and weight. Several authors have described the presence of provirus and infectious virus both in milk and colostrum from most BLV-infected cows and in bulk tank milk ( 58 – 60 ). Thus, both milk and colostrum are sources of infection to neonatal calves.…”

Section: Transmissionmentioning

confidence: 99%

Bovine Leukemia Virus Infection in Neonatal Calves. Risk Factors and Control Measures

et al. 2018

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Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). Although efficient eradication programs have been successfully implemented in most European countries and Oceania, BLV infection rates are still high worldwide. BLV naturally infects cattle, inducing a persistent infection with diverse clinical outcomes. The virus infects lymphocytes and integrates a DNA intermediate as a provirus into the genome of the cells. Therefore, exposure to biological fluids contaminated with infected lymphocytes potentially spreads the virus. Vertical transmission may occur in utero or during delivery, and about 10% of calves born to BLV-infected dams are already infected at birth. Most frequently, transmission from dams to their offspring occurs through the ingestion of infected colostrum or milk. Therefore, although EBL is not a disease specific to the neonatal period, during this period the calves are at special risk of becoming infected, especially in dairy farms, where they ingest colostrum and/or raw milk either naturally or artificially. Calves infected during the first week of life could play an active role in early propagation of BLV to susceptible animals. This review discusses the main factors that contribute to neonatal BLV infection in dairy herds, as well as different approaches and management practices that could be implemented to reduce the risk of BLV transmission during this period, aiming to decrease BLV infection in dairy herds.

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Section: Transmissionmentioning

confidence: 99%

Bovine Leukemia Virus Infection in Neonatal Calves. Risk Factors and Control Measures

et al. 2018

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“…Previous studies report the presence of BLV in the bovine mammary epihtelium (Buehring et al, 1994;Yoshikawa et al, 1997). However, most recent works are focused in the detection of BLV infected mononuclear cells in milk (Jaworski et al, 2016;Kuckleburg et al, 2003), and its potential risk to human (Olaya-Galán et al, 2017;Sellers et al, 2008).…”

mentioning

confidence: 99%

Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV)

et al. 2018

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Bovine leukemia virus (BLV) is a retrovirus that affects cattle causing a lymphoproliferative disease. BLV infection has been associated with misbalance of the immune response causing a higher incidence of other infections. Mastitis is one of the most important conditions that affect milk production in cattle. The aim of this study was to stably infect a bovine mammary epithelial cell line (MAC-T). MAC-T cell line was successfully infected with BLV and the infection was confirmed by nested PCR, qPCR, immunocytochemistry, western blot and transmission electron microscopy. This is the first report of a bovine mammary epithelial cell line stably infected with BLV. This new cell line could be used as an in vitro model to study the effect of BLV on the immune response in the mammary gland and the relationship with other agents causing mastitis.

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“…Serological research methods are the basis for the diagnosis of leukemia in cattle at the present stage. The most used and legal methods for diagnosing cattle leukemia are agar gel immunodiffusion (AGID) and enzyme immunoassay (ELISA) (Jaworski et al, 2016). The basis of these methods is the interaction of speci ic precipitating antibodies with the BLV antigens, which can be detected 3-6 weeks after infection.…”

Section: Introductionmentioning

confidence: 99%

Features of humoral immunity in cows infected with the leukaemia virus

Yakupov

Valiev

Zinnatov

et al. 2020

ijrps

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The article presents the results of studying the dynamics of the formation of antibodies and immune complexes, reveals the prospects for improving the early diagnosis of cattle leukaemia. Studies were conducted for 6 months on an experimental group of animals consisting of 12 cows. The titers of free and bound antibodies in blood serum and milk were determined by enzyme-linked immunosorbent assay. The results of studies showing that changes in titers of anti-leukaemia antibodies in the blood serum of cows naturally infected with BLV (bovine leukaemia virus) are significantly different from experimental infection data are adduced. In cows infected with BLV, there is no definite relationship between antibody titers in milk and in blood serum. With sufficiently high titers of serum antibodies, antibody titers in milk can be minimal for the same cows; conversely, with low titers of serum antibodies, there can be high antibody titers in milk. In the titers of antibodies free and bound in the immune complexes in blood serum with the development of the disease, a certain dependence is traced. With a decrease in titers of free antibodies, in most cases, an increase in titers of “bound” antibodies is observed, i.e., the degree of formation of circulating immune complexes (CECs) increases. There is no clearly defined dependence in the dynamics of changes in titers free and bound in immune complexes of antibodies in milk samples. They can remain at the same level for a long time, both at low and rather high levels.

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Short communication: Relationship between the level of bovine leukemia virus antibody and provirus in blood and milk of cows from a naturally infected herd

Cited by 20 publications

References 24 publications

Bovine Leukemia Virus Infection in Neonatal Calves. Risk Factors and Control Measures

Bovine Leukemia Virus Infection in Neonatal Calves. Risk Factors and Control Measures

Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV)

Features of humoral immunity in cows infected with the leukaemia virus

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