2012
DOI: 10.1016/j.molcel.2012.05.036
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Short Structured RNAs with Low GC Content Are Selectively Lost during Extraction from a Small Number of Cells

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Cited by 202 publications
(160 citation statements)
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“…It should be noted that RNA extraction from a small number of cells by organic extraction method could be selectively inefficient for small structured RNAs, such as tRNAs. 29 Thus, total RNA for FL-PCR quantification should be extracted from high amount of cells or identical number of cells when comparing mature tRNA levels in different samples.…”
Section: Resultsmentioning
confidence: 99%
“…It should be noted that RNA extraction from a small number of cells by organic extraction method could be selectively inefficient for small structured RNAs, such as tRNAs. 29 Thus, total RNA for FL-PCR quantification should be extracted from high amount of cells or identical number of cells when comparing mature tRNA levels in different samples.…”
Section: Resultsmentioning
confidence: 99%
“…Tissue samples were removed from storage and placed in 1 mL TRI Reagent ® solution supplemented with the addition of 10 m m MgCl 2 to aid recovery of microRNAs for future analysis (Kim et al ., 2012). Samples were then completely homogenized (15mins for spleen samples, 30mins for muscle samples) using a bead mill (Retsch Technology GmbH, Haan, Germany).…”
Section: Methodsmentioning
confidence: 99%
“…Indeed, the effect of extraction technique on miRNA expression detection has been previously reported by others. 33 The identification of deregulated miRNAs common to both tumor sets, despite the different methodologies employed, adds weight to the evidence that they play a role in thyroid cancer.…”
Section: Modern Pathology (2015) 28 748-757mentioning
confidence: 99%