Short tandem repeat polymorphism linkage to the androgen receptor gene in prostate carcinoma

Abstract

BACKGROUND Due to high polymorphism, common sequences, and ubiquitous presence, short tandem repeats (STRs) may enhance genomic typing to determine prostate carcinoma (CaP) predisposition. The human phosphoglycerate kinase (PGK1) gene is located within Xq11‐Xq13, a region implicated in familial prostate carcinoma, androgen insensitivity, perineal hypospadias, and other genitourinary abnormalities. The PGK1 STR is the most polymorphic site described in the Xq11‐Xq13 interval and was investigated for its ability to detect differences comparing a heterogeneous CaP population versus controls. METHODS We compared PGK1 STR allele sizes in 103 localized CaP patients with 299 control subjects to evaluate the STR's ability to detect potential CaP predisposing genetic factors. Allele sizes were measured with an automated DNA sequencer after polymerase chain reaction (PCR) based copying of the PGK1 STR region. Allele sizes were compared using chi square and Mann‐Whitney U tests. RESULTS Among 402 subjects, there were 10 distinct allele sizes consisting of five common and five relatively rare alleles. The PGK1 STR, 12 allele (12 tetrameric repeats) was more common among patients with CaP (p=0.03). Allele 13 was more common in CaP patients > 60 years old than among younger patients (p< 0.005). CONCLUSIONS Our findings suggest that STRs in the Xq11‐Xq13 region and other regions may provide a means to rapidly scan genetic loci in large populations of CaP patients and controls. Within limitations, STRs offer the advantage of relatively uniform protocols that could potentially provide a means to comprehensively scan genomes at known predisposing loci. Cancer 2001;92:2603–8. © 2001 American Cancer Society.

A Glycolytic Mechanism Regulating an Angiogenic Switch in Prostate Cancer

“…PGK1 is located in a chromosomal region associated with familial prostate cancers, hypospadias, and androgen insensitivity (X chromosome; Xq11-Xq13; ref. 7). In the nucleus, PGK1 influences DNA replication and repair.…”

Epigenetic deregulation of miR-29a and miR-1256 by isoflavone contributes to the inhibition of prostate cancer cell growth and invasion

“…38,39 Therefore, isoflavone with its specific targeting effect on was found in PCa. 29,30 In our study, we found that the expression of PGK-1 was upregulated and the expression of miR-1256 was downregulated in PCa cells and in human prostate tumor tissues. Transfection of miR-1256 mimic into PCa cells inhibited the expression of PGK-1, suggesting that PGK-1 could be a target of miR-1256.…”

Short tandem repeat polymorphism in a novel esophageal cancer‐related gene (ECRG2) implicates susceptibility to esophageal cancer in Chinese population

“…22 The expansion and instability of STRs, especially the trimeric repeats, in the coding region of disease related genes have been thought to be the directed and major reason for many neurological disorders, for example, Huntington disease, Ataxia, Kennedy disease, SCA-1, SCA-3 and Smith disease, et al 23,24 There are several reports about the association of STRs in the prostate cancer related genes with the risk of the cancer, and on the bases of the joint effect of several loci, people want to construct a risk profile for the diagnosis, treatment and prognosis of prostate cancer ultimately. 16,25,26 The prevalence of the somatic instability for polymorphism of tetra-and penta-nucleotide STRs in gastric cancer has been studied, 27 but there is no report about the STRs in esophageal cancer related genes. Our findings of TCA STR in ECRG2 and the polymorphism frequencies in population may give clues for a pilot study of carcinogenesis and development of esophageal cancer.…”

“…15 Owing to high polymorphism, common sequences and ubiquitous presence, STRs were also used in detecting predisposition to tumors, such as prostate cancer. 16 However, to our best …”

“…15 Owing to high polymorphism, common sequences and ubiquitous presence, STRs were also used in detecting predisposition to tumors, such as prostate cancer. 16 However, to our best Abbreviations: CIs, confidence intervals; DHPLC, denaturing high-performance liquid chromatography; ECRG2, esophageal cancer related gene 2; ESCC, esophageal squamous cell carcinoma; ORs, odds rations; PCR, polymerase chain reaction; STR, short tandem repeat.…”