Shortened and multivalent aptamers for ultrasensitive and rapid detection of alternariol in wheat using optical waveguide sensors

Wang, Shuo; Gao, Han; Wei, Zhenzhe; Zhou, Jianshuo; Ren, Shang; He, Junlin; Luan, Yunxia; Lou, Xinhui

doi:10.1016/j.bios.2021.113702

Cited by 29 publications

(19 citation statements)

References 42 publications

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“…They have the characteristics of high stability, no immunogenicity, and easy synthesis and modification, therefore have been proposed as a substitute for conventional antibodies ( Trinh et al, 2021 ; Zhang H. et al, 2017 ). However, the current aptamer-based small molecule sensing platforms are mostly designed using aptamers screened from SELEX, and the original aptamer length is 40–100 nt ( Wang et al, 2022 ). Only a few fractions of bases in aptamer are involved in target binding ( Chinnappan et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

Qin

Bubiajiaer

et al. 2022

Front. Bioeng. Biotechnol.

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Various biosensors based on aptamers are currently the most popular rapid detection approaches, but the performance of these sensors is closely related to the affinity of aptamers. In this work, a strategy for constructed high-affinity aptamer was proposed. By truncating the bases flanking the 59 nt dexamethasones (DEX) original aptamer sequence to improve the sensitivity of the aptamer to DEX, and then base mutation was introduced to further improve the sensitivity and selectivity of aptamers. Finally, the 33 nt aptamer Apt-M13 with G-quadruplex structures was obtained. The dissociation constant (Kd) was determined to be 200 nM by Graphene oxide (GO)-based fluorometry. As-prepared Apt-M13 was used for a label-free colorimetric aptamer sensor based on gold nanoparticles, the LOD was 3.2-fold lower than the original aptamer described in previous works. The anti-interference ability of DEX analogs is also further improved. It indicates that truncation technology effectively improves the specificity of the aptamer to DEX in this work, and the introduction of mutation further improves the affinity and selectivity of the aptamer to DEX. Therefore, the proposed aptamer optimization method is also expected to become a general strategy for various aptamer sequences.

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Section: Introductionmentioning

confidence: 99%

Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

Qin

Bubiajiaer

et al. 2022

Front. Bioeng. Biotechnol.

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“…The modification method of the optical fiber is the same as that reported previously by our group [ 34 , 35 , 37 ]. Briefly, a razor blade was used to strip 3.5 cm of the resin layer at one end of the fiber.…”

Section: Methodsmentioning

confidence: 99%

“…The NADL consists of an aptamer layer and a microextraction layer, which can achieve in situ target purification, detection and in situ target enrichment. We demonstrated the ultrasensitive detection of alternariol spiked in the wheat flour [ 35 ]. The application of EWOF aptasensor for the detection of ZEN has never been achieved.…”

Section: Introductionmentioning

confidence: 99%

Evanescent Wave Optical-Fiber Aptasensor for Rapid Detection of Zearalenone in Corn with Unprecedented Sensitivity

et al. 2022

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Zearalenone (ZEN) is a common mycotoxin pollutant found in agricultural products. Aptamers are attractive recognition biomolecules for the development of mycotoxin biosensors. Even though numerous aptasensors have been reported for the detection of ZEN in recent years, many of them suffer from problems including low sensitivity, low specificity, tedious experimental steps, high-cost, and difficulty of automation. We report here the first evanescent wave optical-fiber aptasensor for the detection of ZEN with unprecedented sensitivity, high specificity, low cost, and easy of automation. In our aptasensor, a 40-nt ZEN-specific aptamer (8Z31) is covalently immobilized on the fiber. The 17-nt fluorophore Cy5.5-labeled complementary DNA strand and ZEN competitively bind with the aptamer immobilized on the fiber, enabling the signal-off fluorescent detection of ZEN. The coating of Tween 80 enhanced both the sensitivity and the reproducibility of the aptasensor. The sensor was able to detect ZEN spiked-in the corn flour extract with a semilog linear detection range of 10 pM-10 nM and a limit of detection (LOD, S/N = 3) of 18.4 ± 4.0 pM (equivalent to 29.3 ± 6.4 ng/kg). The LOD is more than 1000-fold lower than the maximum ZEN residue limits set by China (60 μg/kg) and EU (20 μg/kg). The sensor also has extremely high specificity and showed negligible cross-reactivity to other common mycotoxins. In addition, the sensor was able to be regenerated for 28 times, further decreasing its cost. Our sensor holds great potential for practical applications according to its multiple compelling features.

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“…In addition, the high cost and a long reaction time are also the challenges of using antibodies as the recognition probes . Alternatively, aptamers are synthetic short-chain oligonucleotide fragments that can specifically recognize various species including proteins, cells, and small molecules. , Compared with antibodies or receptor proteins, aptamers possess higher stability, lower cost, and easier preparation and modification procedures. Since Han et al first designed PAEs aptamers and applied them for the detection of PAEs, the sensors based on aptamers have been developed for the detection of PAEs …”

Section: Introductionmentioning

confidence: 99%

Competitive Displacement Triggering DBP Photoelectrochemical Aptasensor via Cetyltrimethylammonium Bromide Bridging Aptamer and Perovskite

Shen

Guan

et al. 2022

Anal. Chem.

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Here, a label-free perovskite-based photoelectrochemical (PEC) aptasensor was rationally designed for the displacement assay of dibutyl phthalate (DBP), a well-known endocrine disruptor, with the aid of cetyltrimethylammonium bromide (CTAB). In this method, CTAB significantly enhanced the PEC response and humidity resistance of the CH3NH3PbI3 perovskite by forming a protecting layer and passivating the X- and A-sites vacancies of CH3NH3PbI3. In addition, CTAB facilitated the immobilization of an aptamer through van der Waals and hydrophobicity forces, as well as the electrostatic interactions between the phosphate group of the aptamer and the cationic group of CTAB. When exposed to DBP in the affinity solution, the DBP aptamer was released from the electrode because the affinity between DBP and its aptamer competes with the interaction of the aptamer and CTAB. The displacement of the aptamer from the perovskite surface relieves the block effect and thus enhances the photoelectric signal of perovskite. By virtue of the good photoelectrochemical characters of CH3NH3PbI3 and the specific recognition ability of aptamer, the linear range of the PEC sensor was 1.0 × 10–13 to 1.0 × 10–8 M and the detection and quantification limits were down to 2.5 × 10–14 and 8.2 × 10–14 M (S/N = 3), respectively. This work offers a novel strategy for designing aptasensors for the detection of various targets and exhibits the marvelous potential of organic–inorganic perovskite in the field of PEC analysis.

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Shortened and multivalent aptamers for ultrasensitive and rapid detection of alternariol in wheat using optical waveguide sensors

Cited by 29 publications

References 42 publications

Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

Engineering constructed of high selectivity dexamethasone aptamer based on truncation and mutation technology

Evanescent Wave Optical-Fiber Aptasensor for Rapid Detection of Zearalenone in Corn with Unprecedented Sensitivity

Competitive Displacement Triggering DBP Photoelectrochemical Aptasensor via Cetyltrimethylammonium Bromide Bridging Aptamer and Perovskite

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