Should I stay or should I go? Traffic control for plant pattern recognition receptors

Frescatada-Rosa, Márcia; Robatzek, Silke; Kühn, Harald

doi:10.1016/j.pbi.2015.08.007

Cited by 26 publications

(23 citation statements)

References 55 publications

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“…Protein accumulation of PRRs is also affected by endocytosis (Ben Khaled et al, 2015;Frescatada-Rosa et al, 2015;Couto and Zipfel, 2016). flg22 induces FLS2 internalization and degradation in Arabidopsis (Robatzek et al, 2006).…”

Section: Dynamic Control Of Prr Complexesmentioning

confidence: 99%

“…Similarly, Cf-4-and Cf-9-mediated resistance and endocytosis in tomato plants require ligand-induced recruitment of BAK1, indicating that diverse classes of PRRs share similar requirements for initiation of resistance and endocytosis (Postma et al, 2016). Readers are referred to recent reviews for detailed discussion on the endocytic regulation of plant RKs and RPs (Ben Khaled et al, 2015;Frescatada-Rosa et al, 2015).…”

Section: Dynamic Control Of Prr Complexesmentioning

confidence: 99%

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Receptor Kinases in Plant-Pathogen Interactions: More Than Pattern Recognition

2017

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Section: Dynamic Control Of Prr Complexesmentioning

confidence: 99%

Section: Dynamic Control Of Prr Complexesmentioning

confidence: 99%

Receptor Kinases in Plant-Pathogen Interactions: More Than Pattern Recognition

2017

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“…RLK/BAK1 complex formation is followed by the transphosphorylation of both kinase domains, the initiation of downstream signalling and the internalization of the activated RLK/BAK1 complex through endocytosis (Couto and Zipfel, ; Frescatada‐Rosa et al. , ; Schwessinger et al. , ; Wang et al.…”

Section: Introductionmentioning

confidence: 99%

Kinase activity of SOBIR1 and BAK1 is required for immune signalling

Burgh

Postma

Robatzek

et al. 2019

Molecular Plant Pathology

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Summary Leucine‐rich repeat‐receptor‐like proteins (LRR‐RLPs) and LRR‐receptor‐like kinases (LRR‐RLKs) trigger immune signalling to promote plant resistance against pathogens. LRR‐RLPs lack an intracellular kinase domain, and several of these receptors have been shown to constitutively interact with the LRR‐RLK Suppressor of BIR1‐1/EVERSHED (SOBIR1/EVR) to form signalling‐competent receptor complexes. Ligand perception by LRR‐RLPs initiates recruitment of the co‐receptor BRI1‐Associated Kinase 1/Somatic Embryogenesis Receptor Kinase 3 (BAK1/SERK3) to the LRR‐RLP/SOBIR1 complex, thereby activating LRR‐RLP‐mediated immunity. We employed phosphorylation analysis of in planta ‐produced proteins, live cell imaging, gene silencing and co‐immunoprecipitation to investigate the roles of SOBIR1 and BAK1 in immune signalling. We show that Arabidopsis thaliana ( At ) SOBIR1, which constitutively activates immune responses when overexpressed in planta , is highly phosphorylated . Moreover, in addition to the kinase activity of SOBIR1 itself, kinase‐active BAK1 is essential for At SOBIR1‐induced constitutive immunity and for the phosphorylation of At SOBIR1. Furthermore, the defence response triggered by the tomato LRR‐RLP Cf‐4 on perception of Avr4 from the extracellular pathogenic fungus Cladosporium fulvum is dependent on kinase‐active BAK1. We argue that, in addition to the trans‐autophosphorylation of SOBIR1, it is likely that SOBIR1 and BAK1 transphosphorylate, and thereby activate the receptor complex. The signalling‐competent cell surface receptor complex subsequently activates downstream cytoplasmic signalling partners to initiate RLP‐mediated immunity.

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“…Besides RbohD (Kadota et al ., ; Li et al ., ), this may also affect the multidrug exporter DTX18 (Dobritzsch et al ., ). It should be noted that the proposed flg22‐triggered delay in CME of plasma membrane proteins appears to contrast with reported flg22‐induced endocytosis of FLS2 (Robatzek et al ., ; Ben Khaled et al ., ; Frescatada‐Rosa et al ., ). It is possible that the endocytosis of FLS2 occurs spatially and/or temporally separately from that of other plasma membrane cargoes, or that the CME of FLS2 might not require PtdIns(4,5)P 2 formed by PIP5K6.…”

Section: Discussionmentioning

confidence: 97%

A PAMP‐triggered MAPK cascade inhibits phosphatidylinositol 4,5‐bisphosphate production by PIP5K6 in Arabidopsis thaliana

et al. 2019

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Summary The phosphoinositide kinase PIP5K6 has recently been identified as a target for the mitogen‐activated protein kinase (MAPK) MPK6. Phosphorylation of PIP5K6 inhibited the production of phosphatidylinositol 4,5‐bisphosphate (PtdIns(4,5)P2), impacting membrane trafficking and cell expansion in pollen tubes. Here, we analyzed whether MPK6 regulated PIP5K6 in vegetative Arabidopsis cells in response to the pathogen‐associated molecular pattern (PAMP) flg22. Promoter‐β‐glucuronidase analyses and quantitative real‐time reverse transcription polymerase chain reaction data show PIP5K6 expressed throughout Arabidopsis tissues. Upon flg22 treatment of transgenic protoplasts, the PIP5K6 protein was phosphorylated, and this modification was reduced for a PIP5K6 variant lacking MPK6‐targeted residues, or in protoplasts from mpk6 mutants. Upon flg22 treatment of Arabidopsis plants, phosphoinositide levels mildly decreased and a fluorescent reporter for PtdIns(4,5)P2 displayed reduced plasma membrane association, contrasting with phosphoinositide increases reported for abiotic stress responses. Flg22 treatment and chemical induction of the upstream MAPK kinase, MKK5, decreased phosphatidylinositol 4‐phosphate 5‐kinase activity in mesophyll protoplasts, indicating that the flg22‐activated MAPK cascade limited PtdIns(4,5)P2 production. PIP5K6 expression or PIP5K6 protein abundance changed only marginally upon flg22 treatment, consistent with post‐translational control of PIP5K6 activity. PtdIns(4,5)P2‐dependent endocytosis of FM 4‐64, PIN2 and the NADPH‐oxidase RbohD were reduced upon flg22 treatment or MKK5 induction. Reduced RbohD‐endocytosis was correlated with enhanced ROS production. We conclude that MPK6‐mediated phosphorylation of PIP5K6 limits the production of a functional PtdIns(4,5)P2 pool upon PAMP perception.

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Should I stay or should I go? Traffic control for plant pattern recognition receptors

Cited by 26 publications

References 55 publications

Receptor Kinases in Plant-Pathogen Interactions: More Than Pattern Recognition

Receptor Kinases in Plant-Pathogen Interactions: More Than Pattern Recognition

Kinase activity of SOBIR1 and BAK1 is required for immune signalling

A PAMP‐triggered MAPK cascade inhibits phosphatidylinositol 4,5‐bisphosphate production by PIP5K6 in Arabidopsis thaliana

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