SHW1 Interacts with HY5 and COP1, and Promotes COP1-mediated Degradation of HY5 During Arabidopsis Seedling Development

Srivastava, Anjil Kumar; Senapati, Dhirodatta; Srivastava, Archana; Chakraborty, Moumita; Gangappa, Sreeramaiah N.; Chattopadhyay, Sudip

doi:10.1104/pp.15.01184

Cited by 25 publications

(24 citation statements)

References 65 publications

(115 reference statements)

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“…In addition to COP1, SPA1-4 promote degradation of HY5 through COP1 (Saijo et al, 2003;Zhu et al, 2008). Another negative regulator of photomorphogenesis, SHORT HYPOCOTYL UNDER BLUE 1 (SHW1) also enhances HY5 degradation in a COP1dependent manner (Srivastava et al, 2015).…”

Section: Regulation Of Hy5 Is Dynamicmentioning

confidence: 99%

The Multifaceted Roles of HY5 in Plant Growth and Development

2016

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ELONGATED HYPOCOTYL5 (HY5), a member of the bZIP transcription factor family, inhibits hypocotyl growth and lateral root development, and promotes pigment accumulation in a light-dependent manner in Arabidopsis. Recent research on its role in different processes such as hormone, nutrient, abiotic stress (abscisic acid, salt, cold), and reactive oxygen species signaling pathways clearly places HY5 at the center of a transcriptional network hub. HY5 regulates the transcription of a large number of genes by directly binding to cis-regulatory elements. Recently, HY5 has also been shown to activate its own expression under both visible and UV-B light. Moreover, HY5 acts as a signal that moves from shoot to root to promote nitrate uptake and root growth. Here, we review recent advances on HY5 research in diverse aspects of plant development and highlight still open questions that need to be addressed in the near future for a complete understanding of its function in plant signaling and beyond.

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Section: Regulation Of Hy5 Is Dynamicmentioning

confidence: 99%

The Multifaceted Roles of HY5 in Plant Growth and Development

2016

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“…Recombinant protein expression and production in E. coli were previously described (Srivastava et al, 2015). OsOTS1 (GST: OsOTS1), OsbZIP23 (OsbZIP23:His) were expressed in BL21 (DE3) cells and purified using manufacturer's guidelines for GST and histidine affinity purification tags.…”

Section: Recombinant Protein and Gst-pull Down Assaymentioning

confidence: 99%

RiceSUMOproteaseOverly Tolerant to Salt 1targets the transcription factor, OsbZIP23 to promote drought tolerance in rice

et al. 2017

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Conjugation of SUMO (Small Ubiquitin-like Modifier) protein to cellular targets is emerging as a very influential protein modification system. Once covalently bound, SUMO conjugation can change the stability or functionality of its cognate target proteins. SUMO protease can rapidly reverse SUMO conjugation making this modification system highly dynamic. A major factor in the variation of SUMO-target function is the balance between the conjugated/de-conjugated forms. The mechanistic role of these regulatory SUMO proteases in mediating stress responses has not been defined in any crops. In this study, we reveal the role of the SUMO protease, OsOTS1 in mediating tolerance to drought in rice. OsOTS1 depleted transgenic plants accumulate more ABA and exhibit more productive agronomic traits during drought while OsOTS1 overexpressing lines are drought sensitive but ABA insensitive. Drought and ABA treatment stimulates the degradation of OsOTS1 protein indicating that SUMO conjugation is an important response to drought stress in rice achieved through down-regulation of OTS1/2 activity. We reveal that OsOTS1 SUMO protease directly targets the ABA and drought responsive transcription factor OsbZIP23 for de-SUMOylation affecting its stability. OsOTS-RNAi lines show increased abundance of OsbZIP23 and increased drought responsive gene expression while OsOTS1 overexpressing lines show reduced levels of OsbZIP23 leading to suppressed drought responsive gene expression. Our data reveal a mechanism in which rice plants govern ABA-dependant drought responsive gene expression by controlling the stability of OsbZIP23 by SUMO conjugation through manipulating specific SUMO protease levels.

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“…It has been shown that COP1‐mediated degradation of HY5, LAF1 and HFR1 is promoted by SPA1, another negative regulator of the photomorphogenic pathway (Hoecker and Quail, ; Saijo et al ., ; Seo et al ., , ; Jang et al ., ; Yang et al ., 2005a,b; Yang and Wang, ). Recently, it has been shown that SHW1 is also involved in the stimulation of COP1‐mediated degradation of HY5 (Bhatia et al ., ; Srivastava et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Functional interrelation of MYC2 and HY5 plays an important role in Arabidopsis seedling development

et al. 2019

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Arabidopsis MYC2 bHLH transcription factor plays a negative regulatory role in blue light (BL)-mediated seedling development. HY5 bZIP protein works as a positive regulator of multiple wavelengths of light and promotes photomorphogenesis. Both MYC2 and HY5, belonging to two different classes of transcription factors, are the integrators of multiple signaling pathways. However, the functional interrelations of these two transcription factors in seedling development remain unknown. Additionally, whereas HY5-mediated regulation of gene expression has been investigated in detail, the transcriptional regulation of HY5 itself is yet to be understood. Here, we show that HY5 and MYC2 work in an antagonistic manner in Arabidopsis seedling development. Our results reveal that HY5 expression is negatively regulated by MYC2 predominantly in BL, and at various stages of development. On the other hand, HY5 negatively regulates the expression of MYC2 at various wavelengths of light. In vitro and in vivo DNA-protein interaction studies suggest that MYC2 binds to the E-box cis-acting element of HY5 promoter. Collectively, this study demonstrates a coordinated regulation of MYC2 and HY5 in blue-light-mediated Arabidopsis seedling development.

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SHW1 Interacts with HY5 and COP1, and Promotes COP1-mediated Degradation of HY5 During Arabidopsis Seedling Development

Cited by 25 publications

References 65 publications

The Multifaceted Roles of HY5 in Plant Growth and Development

The Multifaceted Roles of HY5 in Plant Growth and Development

RiceSUMOproteaseOverly Tolerant to Salt 1targets the transcription factor, OsbZIP23 to promote drought tolerance in rice

Functional interrelation of MYC2 and HY5 plays an important role in Arabidopsis seedling development

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