Sialic acids are surface components of<i>Sporothrix schenckii</i>yeast forms

Alviano, Celuta Sales; Pereira, M. E. A.; Souza, Wanderley de; Oda, Leila M.; Travassos, Luiz R.

doi:10.1111/j.1574-6968.1982.tb00072.x

Cited by 35 publications

(11 citation statements)

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“…However, it remains to be addressed whether these enzymes participate in elaboration of glycoproteins and/or glycolipids. Sialic acid has previously been reported as a component of S. schenckii cell wall glycolipids [96], so it is likely that the putative Golgi CMP-sialic acid transporter is involved in modification of such lipids.…”

Section: Resultsmentioning

confidence: 99%

Comparative genomics of the major fungal agents of human and animal Sporotrichosis: Sporothrix schenckii and Sporothrix brasiliensis

et al. 2014

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BackgroundThe fungal genus Sporothrix includes at least four human pathogenic species. One of these species, S. brasiliensis, is the causal agent of a major ongoing zoonotic outbreak of sporotrichosis in Brazil. Elsewhere, sapronoses are caused by S. schenckii and S. globosa. The major aims on this comparative genomic study are: 1) to explore the presence of virulence factors in S. schenckii and S. brasiliensis; 2) to compare S. brasiliensis, which is cat-transmitted and infects both humans and cats with S. schenckii, mainly a human pathogen; 3) to compare these two species to other human pathogens (Onygenales) with similar thermo-dimorphic behavior and to other plant-associated Sordariomycetes.ResultsThe genomes of S. schenckii and S. brasiliensis were pyrosequenced to 17x and 20x coverage comprising a total of 32.3 Mb and 33.2 Mb, respectively. Pair-wise genome alignments revealed that the two species are highly syntenic showing 97.5% average sequence identity. Phylogenomic analysis reveals that both species diverged about 3.8-4.9 MYA suggesting a recent event of speciation. Transposable elements comprise respectively 0.34% and 0.62% of the S. schenckii and S. brasiliensis genomes and expansions of Gypsy-like elements was observed reflecting the accumulation of repetitive elements in the S. brasiliensis genome. Mitochondrial genomic comparisons showed the presence of group-I intron encoding homing endonucleases (HE’s) exclusively in S. brasiliensis. Analysis of protein family expansions and contractions in the Sporothrix lineage revealed expansion of LysM domain-containing proteins, small GTPases, PKS type1 and leucin-rich proteins. In contrast, a lack of polysaccharide lyase genes that are associated with decay of plants was observed when compared to other Sordariomycetes and dimorphic fungal pathogens, suggesting evolutionary adaptations from a plant pathogenic or saprobic to an animal pathogenic life style.ConclusionsComparative genomic data suggest a unique ecological shift in the Sporothrix lineage from plant-association to mammalian parasitism, which contributes to the understanding of how environmental interactions may shape fungal virulence. . Moreover, the striking differences found in comparison with other dimorphic fungi revealed that dimorphism in these close relatives of plant-associated Sordariomycetes is a case of convergent evolution, stressing the importance of this morphogenetic change in fungal pathogenesis.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-943) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Comparative genomics of the major fungal agents of human and animal Sporotrichosis: Sporothrix schenckii and Sporothrix brasiliensis

et al. 2014

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“…Remarkably, in the fungi Sporothrix schenkii [926] and Fonsecaea pedrosi [928], both of which are pathogenic to man, NeuSAc and NeuSGc were found. In this way, cells and molecules are protected against damaging influences from the environment, such as proteases and immunological or phagocytotic agents.…”

Section: 2 Sialic Acids Masking Biological Recognition Sitesmentioning

confidence: 99%

Chemistry, biochemistry and biology of sialic acids

Schauer

Kamerling

1997

New Comprehensive Biochemistry

168

228

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“…Their presence in the pathogenic fungi Sporothrix schenckii and Fonsecaea pedrosoi has been suggested [3,4] based on a combination of evidence. In S. schenckii, treatment of yeast forms with neuraminidase increased about 8-fold their uptake by mouse peritoneal macrophages [5], suggesting that sialic acid units may protect the fungus against phagocytosis before an effective antibody response is established in the infected host [6].…”

Section: Brasiliensismentioning

confidence: 99%

Identification of sialic acids on the cell surface of hyphae and yeast forms of the human pathogen Paracoccidioides brasiliensis

Soares

1993

FEMS Microbiology Letters

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Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis, when grown in a synthetic medium, expresses at the cell surface of both yeast and mycelial forms acidic glycoconjugates containing N-acetylneuraminic acid units. Sialic acids were extracted using mild hydrolytic conditions, and were identified by thin-layer and gas chromatography, standard colorimetry, reaction with periodate-resorcinol and mass spectrometry. Their surface location was inferred from fluorescent-lectin (Limulus polyphemus agglutinin) binding to whole cells abrogated by previous treatment with neuraminidase. Expression of sialic acids on virulent yeast forms of P. brasiliensis (3.7 x 10(6) residues per cell) may inhibit fungal phagocytosis during early infection, when the immunological response is still being built up.

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Sialic acids are surface components ofSporothrix schenckiiyeast forms

Cited by 35 publications

References 12 publications

Comparative genomics of the major fungal agents of human and animal Sporotrichosis: Sporothrix schenckii and Sporothrix brasiliensis

Comparative genomics of the major fungal agents of human and animal Sporotrichosis: Sporothrix schenckii and Sporothrix brasiliensis

Chemistry, biochemistry and biology of sialic acids

Identification of sialic acids on the cell surface of hyphae and yeast forms of the human pathogen Paracoccidioides brasiliensis

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