Sıçan Eritrositlerinde Karbon Tetraklorürün(CCl4) ve Kuersetinin Bazı Metabolik Enzim Aktiviteleri Üzerine İn Vivo Etkisinin İncelenmesi

Abstract

In the study; the purpose was to investigate the in vivo impact of carbon tetrachloride (CCl4) and quercetin (Qu) on activities of important metabolic enzymes such as Glucose 6phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), glutathione reductase (GR) and glutathione S-transferase (GST) in rat erythrocytes. At the experimental stage, rats were divided into 4 groups. 1.Group (Control): Pure olive oil at a dose determined according to their body weight (1mL/kg) was given to the rats in this group, 2.Group (CCl4: 1.0 mL/kg (ip)(1:1), 3.Group (Ku: 25) mg/kg (ip), 4.Group (CCl4(1.0 ml/kg (ip)+ Ku (25 mg/kg (ip) was injected. The study was continued for 3 days. The results revealed that the activities of; G6PD (p˂0.01), 6PGD (p˂0.01), GR(p˂0.001) and GST (p˃0.05) enzyme activities were decreased in the CCl4 group compared to the control group. It was determined that enzyme activities were higher in CCl4+Qu applied groups compared to CCl4 group. The application of Qu caused an increase in the enzyme activity value. This can be accepted as an indication that the inhibition caused by CCI4 has disappeared. Consequently; It is thought that Qu may be effective in preventing oxidative damage due to CCl4 administration.