Sickle-cell membrane-bound (Ca2+ + Mg2+)-ATPase: Activation by 3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-6-butyric acid, a novel antisickling agent

Bewaji, Clement O.; Olorunsogo, O. O.; Bababunmi, Enitan A.

doi:10.1016/0143-4160(85)90009-0

Cited by 8 publications

(8 citation statements)

References 24 publications

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“…For Mg 2+ -ATPase, the level was significantly (p<0.05) higher in HbSS than HbAA and HbAS erythrocytes. These findings agree with earlier report on the relative activities of the three ATPases in human erythrocytes of different genotypes (4,8,20).…”

Section: Resultssupporting

confidence: 93%

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In vitro effects of aqueous extracts of Zanthoxylum macrophylla roots on adenosines triphosphatases from human erythrocytes of different genotypes

2005

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The in vitro effects of aqueous extracts of Zanthoxylum macrophylla roots on the activities of three ATPases (Na + , K + -; Ca 2+ -; and Mg 2+ -ATPases) were investigated in membrane preparations from human erythrocytes of HbAA, HbAS and HbSS bloods. The activities of these membrane-bound enzymes were determined in the presence of varying concentrations of the plant extract. The results indicated that for Na + , K + -and Ca 2+ -ATPases, a dose-dependent activation of the three enzymes by the aqueous plant extract occurred. The trend for the different genotypes was HbSS>HbAS>HbAA. The activations were significant (p<0.05) when compared with activities obtained in the absence of the plant extract. Phenylalanine, a known anti-sickling agent, at varying concentrations (200-800µM) produced similar activating effect. In contrast, the extract inhibited the Mg 2+ -ATPase activity in a dose-dependent manner with a trend of HbSS>HbAS>HbAA. The inhibitions seen were significant (p<0.05) in comparison with activities in the absence of the plant extract. Phenylalanine produced similar effect for Mg 2+ -ATPase. These findings in this study suggest that differences in the ATPases of the different genotypes may account for the reports of other workers on the involvement of ion transport as a factor in the physiological manifestation of sickle cell disease. The findings are also significant to an understanding of the molecular basis for the use of the aqueous extract of Z. macrophylla roots in the management of sickle cell disease.

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Section: Resultssupporting

confidence: 93%

“…In addition, elevated intracellular Ca 2+ had been suggested to destroy the normal cell shape and plasticity leading to increase K + leakage hence inhibiting Na + , K + -pump (7). Indeed, it had been suggested that sickling of red cells could be reversed if the excess Ca 2+ in the red cells is pumped out (8).…”

Section: Introductionmentioning

confidence: 99%

In vitro effects of aqueous extracts of Zanthoxylum macrophylla roots on adenosines triphosphatases from human erythrocytes of different genotypes

2005

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“…The effectiveness of this method has been previously shown in identifying bioactive components in plant extracts. 39,52–55 The results of our tests are listed in the Tables 1–4, where 28, 29, 30, and 29 secondary metabolites were identified from C. cajan leaf, C. cajan seed, Z. zanthoxyloides leaf, and C. papaya aqueous leaf extracts, respectively. Despite the effectiveness of GC–MS analysis in identifying possible secondary metabolites in plant extracts, it cannot be used for classifying the constituents into functional or structural groups, since GC–MS is designed to identify the compounds by comparing the candidates with known molecules in the database/library.…”

Section: Results and Discusionmentioning

confidence: 99%

Potential of Three Ethnomedicinal Plants as Antisickling Agents

et al. 2016

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Sickle cell disease (SCD) is a genetic blood disorder that affects the shape and transportation of red blood cells (RBCs) in blood vessels, leading to various clinical complications. Many drugs that are available for treating the disease are insufficiently effective, toxic, or too expensive. Therefore, there is a pressing need for safe, effective, and inexpensive therapeutic agents from indigenous plants used in ethnomedicines. The potential of aqueous extracts of Cajanus cajan leaf and seed, Zanthoxylum zanthoxyloides leaf, and Carica papaya leaf in sickle cell disease management was investigated in vitro using freshly prepared 2% sodium metabisulfite for sickling induction. The results indicated that the percentage of sickled cells, which was initially 91.6% in the control, was reduced to 29.3%, 41.7%, 32.8%, 38.2%, 47.6%, in the presence of hydroxyurea, C. cajan seed, C. cajan leaf, Z. zanthoxyloides leaf, and C. papaya leaf extracts, respectively, where the rate of polymerization inhibition was 6.5, 5.9, 8.0, 6.6, and 6.0 (×10−2) accordingly. It was also found that the RBC resistance to hemolysis was increased in the presence of the tested agents as indicated by the reduction of the percentage of hemolyzed cells from 100% to 0%. The phytochemical screening results indicated the presence of important phytochemicals including tannins, saponins, alkaloids, flavonoids, and glycosides in all the plant extracts. Finally, gas chromatography–mass spectrometry analysis showed the presence of important secondary metabolites in the plants. These results suggest that the plant extracts have some potential to be used as alternative antisickling therapy to hydroxyurea in SCD management.

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“…Nearly 70% of the world's population (mainly in the developing countries) relied mostly on traditional medicine therapies as their basic way of health care. 1 Andrographis paniculata Nees (Acanthaceae) known as "king of bitters" is used traditionally as a remedy for fever. The aerial parts of the plant are commonly used to treat cold, hypertension, diabetes, cancer, malaria and snake bite.…”

Section: Introductionmentioning

confidence: 99%

Photochemical screening and in vitro antimicrobial analysis of chloroform, methanol and acetone extracts of leaves of Andrographis paniculata

Bassey¹,

Ifeayinwa²,

Onuorah³

et al. 2019

IJMBOA

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Herbs have been used for centuries to improve health and wellbeing of mankind. Mainstream modern science is now beginning to re-focus on herbs and natural plant therapies as a primary means to deal with current day health issues. Plants have been an essential part of human society since the cultivation. The aim of this study focus on the phytochemicals and in vitro analysis of the antimicrobials activities of chloroform, methanol and acetone extracts of Andrographis paniculata leaves. Phytochemical analysis revealed the presence of flavonoids, alkaloids, glycosides, steroids, phenols, tannins and saponins. In vitro antimicrobial effect of these extracts were tested on four clinical and health important microbes (Staphylococcus aureus, Escherichia coli, Aspergillus niger and Candida albicans) at 400, 200, 100, 50 and 25mg/ml concentrations. The agar well diffusion and serial doubling dilution techniques were employed in this analysis, with azithromycin and fluconazole as the positive controls, whereas drug free and extract free solutions of chloroform, methanol and acetone were used as negative controls. All the extracts showed inhibitory effect on all the test organisms when compared with the positive and negative controls. The inhibitory zone diameter ranges from 50 to 200mg/ml whereas the lethal concentrations ranged from 100 to 200mg/ml. The chloroform extract of this plant was more effective than methanol and acetone extracts, and Staphylococcus aureus proved more sensitive to the extracts than E. coli, Aspergillus niger and Candida albicans; nevertheless the plant extracts were considerably effective against all the organisms tested in this study. Therefore, this study justifies the use of Andrographis paniculata as potential source of antibiotics, and recommends the use of highly effective organic solvents for its extraction.

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Sickle-cell membrane-bound (Ca2+ + Mg2+)-ATPase: Activation by 3,4-dihydro-2,2-dimethyl-2H-1-benzopyran-6-butyric acid, a novel antisickling agent

Cited by 8 publications

References 24 publications

<i>In vitro</i> effects of aqueous extracts of <i>Zanthoxylum macrophylla</i> roots on adenosines triphosphatases from human erythrocytes of different genotypes

<i>In vitro</i> effects of aqueous extracts of <i>Zanthoxylum macrophylla</i> roots on adenosines triphosphatases from human erythrocytes of different genotypes

Potential of Three Ethnomedicinal Plants as Antisickling Agents

Photochemical screening and in vitro antimicrobial analysis of chloroform, methanol and acetone extracts of leaves of Andrographis paniculata

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