Sickle Cell Trait (HbAS) is Associated with Increased Expression of Erythrocyte Complement Regulatory Proteins CR1 and CD55 Levels in Children

Otieno, Walter; Estambale, Benson B.; Odera, Michael M.; Gondi, Stacey M. O.; Aluoch, Joash R.; Stoute, A.

doi:10.9734/ijtdh/2013/2948

Cited by 7 publications

(12 citation statements)

References 36 publications

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“…In areas of P. falciparum malaria holoendemicity such as Kisumu in Western Kenya, malaria contributes to 63.4% of admissions in children less than 5 years (12). Due to this protection associated with SCT, these disorders have reached a balanced polymorphism in malaria holo-endemic regions such as in Kisumu County in western Kenya (13,14). It is thought that SCD make the cell susceptible to haemolysis at reduced thresholds of oxygen and any interior or exterior stressors on the RBC can simply make them haemolyse (14).…”

Section: Introductionmentioning

confidence: 99%

Association between haematological parameters and sickle cell genotypes in children with Plasmodium falciparum malaria resident in Kisumu County in western Kenya

Kosiyo

Otieno

Gitaka

et al. 2020

Preprint

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Background: Sickle cell disease (SCD) is a monogenic disorder due to point mutation in the β-globin gene resulting in substitution of Valine for Glutamic acid. The SCD is prevalent in P. falciparum endemic regions such as western Kenya. Carriage of different sickle cell genotypes may influence haematological parameter during malaria. Children resident in malaria holoendemic regions suffer more from malaria-related complications and this is moderated by the presence of the SCD. In the current study, we determined the association between sickle cell genotypes and haematological parameters in children with P. falciparum malaria resident in Kisumu County in Western Kenya.Methodology: Children (n=217, aged 1-192 months) with acute febrile condition were recruited at Jaramogi Oginga Odinga Teaching and Referral Hospital. Chi-square (c2) analysis was used to determine differences between proportions. Differences in haematological parameters were compared across groups using Kruskal Wallis test and between groups using Mann Whitney U test. Multivariate logistic regression analysis controlling for infection status was used to determine the association between sickle cell genotypes and haematological parameters. Results: Using HbAA as the reference group, multivariate logistic regression analysis revealed that carriage of HbSS was associated with reduced haemoglobin [OR=0.310, 95% CI=0.101-0.956, P=0.041], reduced haematocrit [OR=0.318, 95% CI=0.128-0.793, P=0.014], reduced RBC count [OR=0.124, 95% CI=0.045-0.337, P=0.001], reduced MCHC [OR=0.325, 95% CI=0.118-0.892, P=0.029], increased leucocytosis [OR=9.283, 95% CI=3.167-27.210, P=0.001] and reduced monocytosis [OR=0.319, 95% CI=0.123-0.830, P=0.019]. However, carriage of HbAS was only associated with increased micro-platelets [OR=3.629, 95% CI=1.291-8.276, P=0.012]. Conclusion: Results show that carriage of HbSS in children influence the levels of haemoglobin, haematocrit, RBC, MCHC, WBC and Monocytes. Therefore prior knowledge of HbSS should be considered to improve clinical management of haematological alterations during malaria in children.

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Section: Introductionmentioning

confidence: 99%

Association between haematological parameters and sickle cell genotypes in children with Plasmodium falciparum malaria resident in Kisumu County in western Kenya

Kosiyo

Otieno

Gitaka

et al. 2020

Preprint

Self Cite

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“…Kisumu County has seven sub-counties namely Kisumu East, Kisumu West, Kisumu Central, Muhoroni, Seme, Nyando and Nyakach (31). An earlier study in this site reported that the percentage of children aged below 5 years who had the SCT was 17.4% (13). Furthermore, it has been con rmed that despite intensive malaria control approaches, this region show an increasing trend of malaria infection (33).…”

Section: Study Sitementioning

confidence: 79%

“…Plasmodium falciparum is the most deadly and most prevalent species in areas of malaria holo-endemicity including Kisumu in Western Kenya (11,12). Due to this protection associated with SCT, these disorders have reached a balanced polymorphism in malaria holo-endemic regions such as in Kisumu County in western Kenya (13,14). It is thought that SCD make the cell susceptible to haemolysis at reduced thresholds of oxygen and any interior or exterior stressors on the RBC can simply make them haemolyse (14).…”

Section: Introductionmentioning

confidence: 99%

Association Between Haematological Parameters and Sickle Cell Genotypes in Children With Plasmodium Falciparum Malaria Residents in Kisumu County in Western Kenya

Kosiyo

Otieno

Gitaka

et al. 2020

Preprint

Self Cite

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Background: Sickle cell disease (SCD) is a monogenic disorder due to point mutation in the β-globin gene resulting in substitution of Valine for Glutamic acid. The SCD is prevalent in P. falciparum endemic regions such as western Kenya. Carriage of different sickle cell genotypes may influence haematological parameter during malaria. Children resident in malaria holoendemic regions suffer more from malaria-related complications and this is moderated by the presence of the SCD. In the current study, we determined the association between sickle cell genotypes and haematological parameters in children with P. falciparum malaria resident in Kisumu County in Western Kenya.Methodology: Children (n=217, aged 1-192 months) with acute febrile condition were recruited at Jaramogi Oginga Odinga Teaching and Referral Hospital. Chi-square (2) analysis was used to determine differences between proportions. Differences in haematological parameters were compared across groups using Kruskal Wallis test and between groups using Mann Whitney U test. Multivariate logistic regression analysis controlling for infection status was used to determine the association between sickle cell genotypes and haematological parameters. Results: Using HbAA as the reference group, multivariate logistic regression analysis revealed that carriage of HbSS was associated with reduced haemoglobin [OR=0.310, 95% CI=0.101-0.956, P=0.041], reduced haematocrit [OR=0.318, 95% CI=0.128-0.793, P=0.014], reduced RBC count [OR=0.124, 95% CI=0.045-0.337, P=0.001], reduced MCHC [OR=0.325, 95% CI=0.118-0.892, P=0.029], increased leucocytosis [OR=9.283, 95% CI=3.167-27.210, P=0.001] and reduced monocytosis [OR=0.319, 95% CI=0.123-0.830, P=0.019]. However, carriage of HbAS was only associated with increased micro-platelets [OR=3.629, 95% CI=1.291-8.276, P=0.012]. Conclusion: Results show that carriage of HbSS in children influence the levels of haemoglobin, haematocrit, RBC, MCHC, WBC and Monocytes. Therefore prior knowledge of HbSS should be considered to improve clinical management of haematological alterations during malaria in children.

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“…*Fisher’s exact test, § One-way ANOVA. ‡ α + thalassaemia and sickle cell genotype were included in the study as both have previously been reported to influence CR1 copy number 40 , 42 , 63 . SD, standard deviation.…”

Section: Resultsmentioning

confidence: 99%

No Evidence that Knops Blood Group Polymorphisms Affect Complement Receptor 1 Clustering on Erythrocytes

Swann

Harrison

Opi

et al. 2017

Sci Rep

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Clustering of Complement Receptor 1 (CR1) in the erythrocyte membrane is important for immune-complex transfer and clearance. CR1 contains the Knops blood group antigens, including the antithetical pairs Swain-Langley 1 and 2 (Sl1 and Sl2) and McCoy a and b (McCa and McCb), whose functional effects are unknown. We tested the hypothesis that the Sl and McC polymorphisms might influence CR1 clustering on erythrocyte membranes. Blood samples from 125 healthy Kenyan children were analysed by immunofluorescence and confocal microscopy to determine CR1 cluster number and volume. In agreement with previous reports, CR1 cluster number and volume were positively associated with CR1 copy number (mean number of CR1 molecules per erythrocyte). Individuals with the McC b /McC b genotype had more clusters per cell than McC a/McC a individuals. However, this association was lost when the strong effect of CR1 copy number was included in the model. No association was observed between Sl genotype, sickle cell genotype, α+thalassaemia genotype, gender or age and CR1 cluster number or volume. Therefore, after correction for CR1 copy number, the Sl and McCoy polymorphisms did not influence erythrocyte CR1 clustering, and the effects of the Knops polymorphisms on CR1 function remains unknown.

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Sickle Cell Trait (HbAS) is Associated with Increased Expression of Erythrocyte Complement Regulatory Proteins CR1 and CD55 Levels in Children

Cited by 7 publications

References 36 publications

Association between haematological parameters and sickle cell genotypes in children with Plasmodium falciparum malaria resident in Kisumu County in western Kenya

Association between haematological parameters and sickle cell genotypes in children with Plasmodium falciparum malaria resident in Kisumu County in western Kenya

Association Between Haematological Parameters and Sickle Cell Genotypes in Children With Plasmodium Falciparum Malaria Residents in Kisumu County in Western Kenya

No Evidence that Knops Blood Group Polymorphisms Affect Complement Receptor 1 Clustering on Erythrocytes

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