Sidestream dark field videomicroscopy for evaluating liver microcirculation in vivo

Wigmore, Stephen J.; Oniscu, Gabriel C.

doi:10.1002/lt.24734

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…Sidestream dark field imaging is a noninvasive technique for measuring local tissue perfusion. The device emits 530 nm of polarized light or green light to the sublingual tissue, which appears as a dark area in the field of view if the light is absorbed by the hemoglobin in the microvasculature and as a bright area if it is not absorbed (53). The sublingual microcirculation index can be calculated from the data obtained by SDF imaging.…”

Section: Sublingual Microcirculation Parametersmentioning

confidence: 99%

Hemorrhagic Shock Assessed by Tissue Microcirculatory Monitoring: A Narrative Review

Yao,

Chen,

et al. 2023

Shock

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Hemorrhagic shock (HS) is a common complication after traumatic injury. Early identification of HS can reduce patients’ risk of death. Currently, the identification of HS relies on macrocirculation indicators such as systolic blood pressure and heart rate, which are easily affected by the body's compensatory functions. Recently, the independence of the body's overall macrocirculation from microcirculation has been demonstrated, and microcirculation indicators have been widely used in the evaluation of HS. In this study, we reviewed the progress of research in the literature on the use of microcirculation metrics to monitor shock. We analyzed the strengths and weaknesses of each metric and found that microcirculation monitoring could not only indicate changes in tissue perfusion before changes in macrocirculation occurred but also correct tissue perfusion and cell oxygenation after the macrocirculation index returned to normal following fluid resuscitation, which is conducive to the early prediction and prognosis of HS. However, microcirculation monitoring is greatly affected by individual differences and environmental factors. Therefore, the current limitations of microcirculation assessments mean that they should be incorporated as part of an overall assessment of HS patients. Future research should explore how to better combine microcirculation and macrocirculation monitoring for the early identification and prognosis of HS patients.

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Section: Sublingual Microcirculation Parametersmentioning

confidence: 99%

Hemorrhagic Shock Assessed by Tissue Microcirculatory Monitoring: A Narrative Review

Yao,

Chen,

et al. 2023

Shock

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Three-dimensional structure of liver vessels and spatial distribution of hepatic immune cells

Liu

et al. 2023

J. Innov. Opt. Health Sci.

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As the largest internal organ of the human body, the liver has an extremely complex vascular network and multiple types of immune cells. It plays an important role in blood circulation, material metabolism, and immune response. Optical imaging is an effective tool for studying fine vascular structure and immunocyte distribution of the liver. Here, we provide an overview of the structure and composition of liver vessels, the three-dimensional (3D) imaging of the liver, and the spatial distribution and immune function of various cell components of the liver. Especially, we emphasize the 3D imaging methods for visualizing fine structure in the liver. Finally, we summarize and prospect the development of 3D imaging of liver vessels and immune cells.

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Instrumental Non-Invasive Diagnostics of the Depth of Skin Burn: Current Opportunities and Unsolved Problems

Petrova

Сергеевна²,

Немирова

et al. 2021

VECS

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Introduction. Burns of various etiologies are on the 3-4 place in frequency among all injuries, they are associated with a high percentage of complications, disability and mortality. Currently, a single algorithm for objective verification of the depth of skin damage has not been developed. Numerous diagnostic technologies used to solve this problem require systemic analysis.The aim of study was to analyze current literature data on technologies for instrumental diagnostics of the depth of thermal damage to the skin.Materials and methods. A search for literary sources in databases was conducted using eLIBRARY.RU, PubMed, Cyberleninka databases. The period of publications was limited to 2011-2020.Results. The method of objective "gold standard" verification of thermal damage to the skin remains a biopsy followed by morphological examination, but the method's traumaticity does not allow it to be used for monitoring the course of the process in dynamics, for multi-focal studies. Among non-invasive methods, dermatoscopy and video microscopy are noted, but the small imaging area and the need for direct contact of the device with the skin surface limit the use of these methods. Laser Doppler flowmetry and imaging are fairly accurate non-contact methods that allow assessing the condition of a burn wound in real time. The method is not applicable to wounds with blisters, insufficient sanitation. Laser speckle contrast imaging is used to evaluate blood flow and is based on the analysis of speckle structure fluctuations. The method allows determining the degree of burn damage depending on changes in the blood flow. However, the method is difficult to apply with a vessel diameter sized 40 microns. The method is sensitive to movement and internal factors. Infrared and Raman spectroscopy are technique for evaluating vibrational patterns in a particular spectrum, these can be used to identify a molecule or determine its structure. Simultaneous use of spatial frequency visualization and speckle imaging demonstrated a high level of correlation with the results of histological research, which makes the use of these methods promising. Ultrasound also provides a proper correlation with histological data, but it is an imperfect method due to inaccuracies in determining the thickness of the epidermis, dermis, and scar condition. Optical coherence tomography (OCT) is a non-invasive optical method that allows obtaining high-resolution images of skin architecture in real time. The high level of compliance of the visualized structures, in particular, layers, appendages and vessels of the skin, with histological findings, gave this method a name "optical biopsy". Anatomical congruence of normal and damaged skin was established in an experiment between histology and OCT.Conclusion. Early assessment of the depth of thermal damage to the skin is crucial for choosing a personalized treatment strategy for the burned. None of the modern diagnostic methods is universal. Multimodal approaches to diagnostics are the most effective.

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Sidestream dark field videomicroscopy for evaluating liver microcirculation in vivo

Cited by 3 publications

References 7 publications

Hemorrhagic Shock Assessed by Tissue Microcirculatory Monitoring: A Narrative Review

Hemorrhagic Shock Assessed by Tissue Microcirculatory Monitoring: A Narrative Review

Three-dimensional structure of liver vessels and spatial distribution of hepatic immune cells

Instrumental Non-Invasive Diagnostics of the Depth of Skin Burn: Current Opportunities and Unsolved Problems

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