Signal amplification for impedimetric genosensing using gold-streptavidin nanoparticles

Bonanni, Alessandra; Esplandiu, M.J.; Valle, Manel del

doi:10.1016/j.electacta.2007.11.030

Cited by 61 publications

(41 citation statements)

References 47 publications

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“…Regarding the labelled DNA detection schemes, one common strategy includes the chemical labeling of target DNA sequences with redox active molecules, enzymes, nanoparticles, etc (Fan, 2003;Pividori, 2003;He, 2004a;He, 2006;Bonanni, 2007;. In the case of enzymatic labels bound to the DNA target, the enzyme triggers the catalysis of a redox active reaction under hybridization.…”

mentioning

confidence: 99%

Carbon Nanotubes as Suitable Electrochemical Platforms for Metalloprotein Sensors and Genosensors

Pacios¹,

Martin-Fernandez²,

Sarria-Villa³

et al. 2011

Carbon Nanotubes - Growth and Applications

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mentioning

confidence: 99%

Carbon Nanotubes as Suitable Electrochemical Platforms for Metalloprotein Sensors and Genosensors

Pacios¹,

Martin-Fernandez²,

Sarria-Villa³

et al. 2011

Carbon Nanotubes - Growth and Applications

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“…DNA probe and target concentration were fixed at 30 pmol value as studied earlier using the same type of electrode [35]. Experimental conditions such as type of buffers and variation of time required in each step were also optimized in previous work [36].…”

Section: Methodsmentioning

confidence: 99%

Dual‐Genic Hybridization Sensor Employing Electrochemical Impedance Spectroscopy

2008

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“…Sixty microliter of strep-AuNPs, from a 1/100 dilution of the stock solution in phosphate buffer were dropped onto the electrodes [33]. This step was followed by two gentle washing steps in phosphate buffer for 10 min at 25°C.…”

Section: Addition Of Strep-aunpsmentioning

confidence: 99%

Aptamer-antibody sandwich assay for cytochrome c employing an MWCNT platform and electrochemical impedance

2015

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We report on a sensitive aptamer-antibody interaction-based assay for cytochrome c (Cyt c) using electrochemical impedance. 4-Amino benzoic acid is used for the oriented immobilization of aminated aptamers onto multi-walled carbon nanotubes on the surface of a screen-printed electrode via electrochemical grafting. Impedance was measured in a solution containing the redox system ferro/ferricyanide. The change in interfacial charge transfer resistance (R ct ) experienced by the redox marker was recorded to confirm the formation of a complex between aptamer and the target (Cyt c). A biotinylated antibody against cytochrome c was then used in a sandwich type of assay. The addition of streptavidin conjugated to gold nanoparticles and signal enhancement by treatment with silver led to a further increase in Rct. Under optimized conditions, a detection limit as low as 12 pM was obtained. Cross-reactivity against other serum proteins including fibrinogen, BSA and immunoglobulin G demonstrated improved selectivity.

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Signal amplification for impedimetric genosensing using gold-streptavidin nanoparticles

Cited by 61 publications

References 47 publications

Carbon Nanotubes as Suitable Electrochemical Platforms for Metalloprotein Sensors and Genosensors

Carbon Nanotubes as Suitable Electrochemical Platforms for Metalloprotein Sensors and Genosensors

Dual‐Genic Hybridization Sensor Employing Electrochemical Impedance Spectroscopy

Aptamer-antibody sandwich assay for cytochrome c employing an MWCNT platform and electrochemical impedance

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