Signaling Pathways Coupled to Activation of the Kinin B1 Receptor

Ehrenfeld, Pamela; Figueroa, Carlos D.; Bhoola, K. D.; Matus, Carola E.

doi:10.5772/48058

Cited by 2 publications

(2 citation statements)

References 98 publications

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“…Whereas LDBK stimulates secretion of gelatinases from human neutrophils and breast cancer cells by activating ERK1/2 and p38 MAPK signalling pathways, in breast cancer cells this event involves transactivation of epidermal growth factor receptor (EGFR) . LDBK does not signal Ca 2+ mobilization in primary cultured human keratinocytes, but it triggers EGFR transactivation, an event that involves phosphorylation of Tyr845, Tyr992 and Tyr1068 of EGFR . The EGFR transactivation was blocked by AG1478 (tyrphostin), a specific inhibitor of the EGFR tyrosine kinase activity .…”

Section: Introductionmentioning

confidence: 99%

Activation of the human keratinocyte B1 bradykinin receptor induces expression and secretion of metalloproteases 2 and 9 by transactivation of epidermal growth factor receptor

Matus

Ehrenfeld

Pavicic

et al. 2016

Experimental Dermatology

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The B1 bradykinin receptor (BDKRB1) is a component of the kinin cascade localized in the human skin. Some of the effects produced by stimulation of BDKRB1 depend on transactivation of epidermal growth factor receptor (EGFR), but the mechanisms involved in this process have not been clarified yet. The primary purpose of this study was to determine the effect of a BDKRB1 agonist on wound healing in a mouse model and the migration and secretion of metalloproteases 2 and 9 from human HaCaT keratinocytes and delineate the signalling pathways that triggered their secretion. Although stimulation of BDKRB1 induces weak chemotactic migration of keratinocytes and wound closure in an in vitro scratch-wound assay, the BDKRB1 agonist improved wound closure in a mouse model. BDKRB1 stimulation triggers synthesis and secretion of both metalloproteases, effects that depend on the activity of EGFR and subsequent phosphorylation of ERK1/2 and p38 mitogen-activated protein kinases and PI3K/Akt. In the mouse model, immunoreactivity for both gelatinases was concentrated around wound borders. EGFR transactivation by BDKRB1 agonist involves Src kinases family and ADAM17. In addition to extracellular matrix degradation, metalloproteases 2 and 9 regulate cell migration and differentiation, cell functions that are associated with the role of BDKRB1 in keratinocyte differentiation. Considering that BDKRB1 is up-regulated by inflammation and/or by cytokines that are abundant in the inflammatory milieu, more stable BDKRB1 agonists may be of therapeutic value to modulate wound healing.

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Section: Introductionmentioning

confidence: 99%

Activation of the human keratinocyte B1 bradykinin receptor induces expression and secretion of metalloproteases 2 and 9 by transactivation of epidermal growth factor receptor

Matus

Ehrenfeld

Pavicic

et al. 2016

Experimental Dermatology

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“…Padronizou-se assim que a cada amostra coletada fosse realizada a quantificação em espectrofotômetro da quantidade necessária de solução de hipoclorito de Cloro 12% necessária para obtenção de 0,1 a 0,2 mg/L de Cloro livre na solução, considerado suficiente para gerar estresse, sem causar morte da população adicionada (EHRENFELD 1997), utilizando o método DPD colorimétrico (HACH TM DR 4000U).…”

Section: Determinação Do Tempo E Concentração De Cloro Adequadaunclassified

Avaliação da técnica 3Mtm Petrifilm tm para análises microbiológicas em água de consumo humano na região de Campinas

Constantino¹,

Pereira²

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por aceitar ser meu orientador e compreender minha falta de tempo. À Norma Miya e colegas do laboratório de Toxinas Microbianas, Alessandra, Isabela e Rafael por todo o suporte e carinho durante o projeto. Aos amigos do laboratório de Saneamento ambiental Enelton, Fernando e Ligia pela ajuda nas análises físico-química. Aos atenciosos pesquisadores Doutora Neusely Silva, Prof. Dr. Edson Nour e Dr.Romeu Cantúsio pelo suporte no desenvolvimento deste projeto.À amiga Heloisa Ruppert pela revisão estatística e Letícia Feix de Abreu pela revisão de português. À Interlab S.A. pelo fornecimento de reagentes com significativos descontos. À 3M pelo fomento deste projeto, através do Genesis Grant e liberação de 15% do meu tempo profissional para dedicação ao mestrado. E finalmente, ao meu amado marido Bruno Constantino, pela a ajuda com coletas e contagens noturnas, mas, principalmente, pelo suporte e pela paciência com minha ausência.

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Signaling Pathways Coupled to Activation of the Kinin B1 Receptor

Cited by 2 publications

References 98 publications

Activation of the human keratinocyte B1 bradykinin receptor induces expression and secretion of metalloproteases 2 and 9 by transactivation of epidermal growth factor receptor

Activation of the human keratinocyte B1 bradykinin receptor induces expression and secretion of metalloproteases 2 and 9 by transactivation of epidermal growth factor receptor

Avaliação da técnica 3Mtm Petrifilm tm para análises microbiológicas em água de consumo humano na região de Campinas

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