Signalling by Transforming Growth Factor Beta Isoforms in Wound Healing and Tissue Regeneration

Gilbert, Richard; Vickaryous, Matthew K.; Viloria-Petit, Alicia

doi:10.3390/jdb4020021

Cited by 128 publications

(118 citation statements)

References 171 publications

(241 reference statements)

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“…In addition to the three isoform prototypes of TGF-β (TGF-β1, TGF-β2 and TGF-β3), this superfamily also involves activin, inhibin, Bone Morphogenetic Proteins (BMPs), Growth and Differentiation Factors (GDFs), miostatin, nodal, leftis and Mullerian Inhibiting Substance (MIS) [9]. TGF-β is attracted to fibroblasts, keratinocytes and inflammatory cells via chemotaxis.…”

Section: G Transforming Growth Factor-β1mentioning

confidence: 99%

“…TGF-β has several known important functions in the wound healing process. The function of TGF-β is to regulate the proliferation, differentiation, migration, invasion and chemotaxis of epithelial cells, fibroblasts and immune cells (inflammatory phase) of tissue, as well as the proliferation, migration, invasion and maturation of endothelial cells (to produce functional blood vessels) during angiogenesis [1,2,9].…”

Section: G Transforming Growth Factor-β1mentioning

confidence: 99%

“…Alpha granules also contain other TGF-β isoforms, but the number of isoforms is much lower compared to TGF-β1 (4000 TGF-β1: 1 TGF-β2: 10 TGF-β3). Activation of the blood coagulation mechanism by platelets will cause fibrin clumping resulting in haemostasis and formation of ascaffold for inflammatory cell migration to the injured tissue [9]. After haemostasis, TGF-β acts as achemo-attractant and an inflammatory mediator for many immune cell types, including neutrophils and other polymer-phonuclear (PMN) cells (24 to 48 hours post injury) and monocyte cells (48 to 96 hours post injury).…”

Section: Advances In Health Science Research Volumementioning

confidence: 99%

“…Although platelets are said to be very rich in TGF-β1, but in neutrophils, the ratio of TGF-β isoforms is very different from that of platelets with more TGF-β3 (12 TGF-β1: 1 TGF-β2: 34 TGF-β3), indicating that there is a specific difference in TGF-β isoform throughout the wound healing process. In addition, the role of macrophages in triggering formation of granulation and angiogenesis tissue is also stimulated by TGF-β [9].…”

Section: Advances In Health Science Research Volumementioning

confidence: 99%

“…After an injury, the three TGF-β isoforms trigger re-epithelialization and destruction of TGF-β (via the antibody neutralization effect) that disrupts wound closure. However, although TGF-β1 plays a role in stimulating keratinocyte migration, TGF-β3 has no such function [9].…”

Section: Advances In Health Science Research Volumementioning

confidence: 99%

See 4 more Smart Citations

The Role of TGF Beta 1 and PDGF BB in Wound Healing of the Palate

Hanafiah¹,

Poravi²,

Angga³

et al. 2018

Proceedings of the International Dental Conference of Sumatera Utara 2017 (IDCSU 2017)

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Abstract-Oral wound healing is a process that consists of three highly integrated and overlapping phases inflammation, proliferation and tissue remodelling. These phases involve the interaction between many cells and growth factors that work together from the beginning of an injury to complete tissue formation either through regeneration of tissue or by scar formation. At the point of injury, circulating platelets are capable of secreting growth factors needed during the wound healing phases. Growth factors such as transforming growth factor beta-1(TGF-β1) and platelet-derived growth factor-BB (PDGF-BB) involved in oral wound healing stimulates epithelial proliferation and angiogenesis within the injured tissue.Proliferation of epithelial cells in wound healing and formation of new blood vessels (angiogenesis) plays a significant role in healing of keratinized mucosa such as the palate and gingiva. Therefore, TGF-β and PDGF, being one of the major growth factors in oral wound healing is found to play a pivotal role in tissue healing of the palatal mucosa after an injury.

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Section: G Transforming Growth Factor-β1mentioning

confidence: 99%

Section: G Transforming Growth Factor-β1mentioning

confidence: 99%

Section: Advances In Health Science Research Volumementioning

confidence: 99%

Section: Advances In Health Science Research Volumementioning

confidence: 99%

Section: Advances In Health Science Research Volumementioning

confidence: 99%

See 3 more Smart Citations

The Role of TGF Beta 1 and PDGF BB in Wound Healing of the Palate

Hanafiah¹,

Poravi²,

Angga³

et al. 2018

Proceedings of the International Dental Conference of Sumatera Utara 2017 (IDCSU 2017)

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Epitope‐Imprinted Nanoparticles as Transforming Growth Factor‐β3 Sequestering Ligands to Modulate Stem Cell Fate

Teixeira

Domingues

Babo

et al. 2020

Adv Funct Materials

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Growth factors (GFs) are biomolecules with potent biological effects but inherent limitations hinder their potential as therapeutic agents and cell culture supplements in tissue engineering and regenerative medicine (TERM). Biomaterials that sequester endogenous GFs by affinity binding might circumvent such limitations and thus are being increasingly investigated. Here, molecularly imprinted nanoparticles (MINPs) are proposed as specific abiotic ligands for GFs. As a proof of concept, a conformational epitope of transforming growth factor‐β3 (TGF‐β3) is designed and surface imprinted onto polyacrylamide‐based nanoparticles by inverse microemulsion polymerization. It is found that, depending on the polymerization mixture composition, MINPs can recognize and preferentially bind TGF‐β3, either in noncompetitive assays or from a complex human fluid (platelet lysate). Substrates functionalized with MINPs are then used for 2D culture of adipose‐derived stem cells. Remarkably, gene and protein expression profiles show a marked upregulation of SOX‐9, suggesting activation of TGF‐β3 signaling pathways without requiring supplementation with exogenous GF. Likewise, culturing these cells in pellets incorporating MINPs previously incubated with platelet lysate results in higher collagen II‐rich matrix deposition, compared to pellets incorporating non‐imprinted nanoparticles. In summary, results suggest MINPs can be used as cost‐effective, stable, and scalable alternative abiotic GF ligands to guide cell fate in TERM applications.

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Development of a Silk Fibroin‐Small Intestinal Submucosa Small‐Diameter Vascular Graft with Sequential VEGF and TGF‐β1 Inhibitor Delivery for In Situ Tissue Engineering

Liu

Rütten

Buhl

et al. 2023

Macromolecular Bioscience

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Proper endothelialization and limited collagen deposition on the luminal surface after graft implantation plays a crucial role to prevent the occurrence of stenosis. To achieve these conditions, a biodegradable graft with adequate mechanical properties and the ability to sequentially deliver therapeutic agents isfabricated. In this study, a dual‐release system is constructed through coaxial electrospinning by incorporating recombinant human vascular endothelial growth factor (VEGF) and transforming growth factor β1 (TGF‐β1) inhibitor into silk fibroin (SF) nanofibers to form a bioactive membrane. The functionalized SF membrane as the inner layer of the graft is characterized by the release profile, cell proliferation and protein expression. It presents excellent biocompatibility and biodegradation, facilitating cell attachment, proliferation, and infiltration. The core‐shell structure enables rapid VEGF release within 10 days and sustained plasmid delivery for 21 days. A 2.0‐mm‐diameter vascular graft is fabricated by integrating the SF membrane with decellularized porcine small intestinal submucosa (SIS), aiming to facilitate the integration process under a stable extracellular matrix structure. The bioengineered graft is functionalized with the sequential administration of VEGF and TGF‐β1, and with the reinforced and compatible mechanical properties, thereby offers an orchestrated solution for stenosis with potential for in situ vascular tissue engineering applications.

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Signalling by Transforming Growth Factor Beta Isoforms in Wound Healing and Tissue Regeneration

Cited by 128 publications

References 171 publications

The Role of TGF Beta 1 and PDGF BB in Wound Healing of the Palate

The Role of TGF Beta 1 and PDGF BB in Wound Healing of the Palate

Epitope‐Imprinted Nanoparticles as Transforming Growth Factor‐β3 Sequestering Ligands to Modulate Stem Cell Fate

Development of a Silk Fibroin‐Small Intestinal Submucosa Small‐Diameter Vascular Graft with Sequential VEGF and TGF‐β1 Inhibitor Delivery for In Situ Tissue Engineering

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