Signalling pathway in the induction of neurite outgrowth in human mesenchymal stem cells

Chu, Miensheng; Chang, Ching-Fang; Yang, Chih Yu; Bau, Yi-Chi; Ho, Larry L.T.; Hung, Shih‐Chieh

doi:10.1016/j.cellsig.2005.05.018

Cited by 43 publications

(37 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the case of MSCs, cAMP elevators have been shown to stimulate neuron-like morphology. 74,75 However, these morphological changes were not consistent with a real process of transdifferentiation into mature functional neurons. 76,77 Indeed, specific application of dbcAMP to MSCs has been shown to have cytotoxic effects.…”

Section: Discussionmentioning

confidence: 89%

Sustained Delivery of Dibutyryl Cyclic Adenosine Monophosphate to the Transected Spinal Cord Via Oligo [(Polyethylene Glycol) Fumarate] Hydrogels

Rooney

Knight

Madigan

et al. 2011

Tissue Engineering Part A

View full text Add to dashboard Cite

This study describes the use of oligo [(polyethylene glycol) fumarate] (OPF) hydrogel scaffolds as vehicles for sustained delivery of dibutyryl cyclic adenosine monophosphate (dbcAMP) to the transected spinal cord. dbcAMP was encapsulated in poly(lactic-co-glycolic acid) (PLGA) microspheres, which were embedded within the scaffolds architecture. Functionality of the released dbcAMP was assessed using neurite outgrowth assays in PC12 cells and by delivery to the transected spinal cord within OPF seven channel scaffolds, which had been loaded with Schwann cells or mesenchymal stem cells (MSCs). Our results showed that encapsulation of dbcAMP in microspheres lead to prolonged release and continued functionality in vitro. These microspheres were then successfully incorporated into OPF scaffolds and implanted in the transected thoracic spinal cord. Sustained delivery of dbcAMP inhibited axonal regeneration in the presence of Schwann cells but rescued MSC-induced inhibition of axonal regeneration. dbcAMP was also shown to reduce capillary formation in the presence of MSCs, which was coupled with significant functional improvements. Our findings demonstrate the feasibility of incorporating PLGA microsphere technology for spinal cord transection studies. It represents a novel sustained delivery mechanism within the transected spinal cord and provides a platform for potential delivery of other therapeutic agents.

show abstract

Section: Discussionmentioning

confidence: 89%

Sustained Delivery of Dibutyryl Cyclic Adenosine Monophosphate to the Transected Spinal Cord Via Oligo [(Polyethylene Glycol) Fumarate] Hydrogels

Rooney

Knight

Madigan

et al. 2011

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…The induction protocol included growth factors known to induce neural differentiation in MSCs, such as epidermal growth factor, basic fibroblast growth factor, and PDGF [33,43], and N2 cocktail, which is reported to have increased survival of neural primary cultures and differentiation [44]. Cyclic AMP, an important intracellular secondary messenger, is also linked to this process [39,45]. We excluded retinoic acid, a substance that we previously used for neuronal differentiation and not astroglial differentiation.…”

Section: Discussionmentioning

confidence: 99%

Migration of Neurotrophic Factors-Secreting Mesenchymal Stem Cells Toward a Quinolinic Acid Lesion as Viewed by Magnetic Resonance Imaging

et al. 2008

View full text Add to dashboard Cite

show abstract

“…8 The cells were grown in a complete culture medium [CCM: DMEM-low glucose (LG) (Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 10 lg/mL streptomycin] at 378C under 5% CO 2 atmosphere. The medium was changed twice a week and subculture was performed at 1:3 split every week.…”

Section: Cell Lines and Culture Conditionsmentioning

confidence: 99%

Fibronectin and pellet suspension culture promote differentiation of human mesenchymal stem cells into insulin producing cells

Chang

Hsu

Chiou

et al. 2007

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Multipotential mesenchymal stem cells (MSCs) isolated from bone marrow can differentiate into multiple mesenchymal tissues and exhibit a neuronal phenotype under appropriate induction conditions. Methods promoting neural differentiation have been adapted to derive insulin producing cells (IPCs) from embryonic stem cells, but it remains unclear whether neuronal cell-based differentiation method will be able to derive IPCs from MSCs. Using a four-stage differentiation protocol which contains neuronal differentiation factor and IPC-conversion reagent-nicotinamide, the potential of human MSCs to differentiate into IPCs was evaluated by means of reverse transcription-polymerase chain reaction, immunostaining, and functional analysis. MSCs in monolayer spontaneously expressed genes for islet transcription factors, Nkx6.1 and Ngn3, but did not express insulin after treatment in this protocol. Pellet suspension culture and the addition of fibronectin enhanced pancreatic differentiation with increase in insulin and Glut2 gene expression. Switching of cells to high-glucose culture further increased immunostaining for proinsulin and insulin. IPCs secreted insulin in response to elevated glucose concentration, which was regulated by reagents that increase cyclic AMP production or modify calcium influx. Our data suggest that MSCs in the monolayer do not undergo IPC differentiation and pellet suspension culture with fibronectin promotes IPCs derived from MSCs.

show abstract

Signalling pathway in the induction of neurite outgrowth in human mesenchymal stem cells

Cited by 43 publications

References 52 publications

Sustained Delivery of Dibutyryl Cyclic Adenosine Monophosphate to the Transected Spinal Cord Via Oligo [(Polyethylene Glycol) Fumarate] Hydrogels

Sustained Delivery of Dibutyryl Cyclic Adenosine Monophosphate to the Transected Spinal Cord Via Oligo [(Polyethylene Glycol) Fumarate] Hydrogels

Migration of Neurotrophic Factors-Secreting Mesenchymal Stem Cells Toward a Quinolinic Acid Lesion as Viewed by Magnetic Resonance Imaging

Fibronectin and pellet suspension culture promote differentiation of human mesenchymal stem cells into insulin producing cells

Contact Info

Product

Resources

About