Significant increases in serum and plasma concentrations of matrix metalloproteinases 3 and 9 in patients with rapidly destructive osteoarthritis of the hip

Abstract

Objective. Rapidly destructive osteoarthritis (OA) of the hip is an uncommon subset of OA that affects mainly elderly women. Previous studies indicate that elevated levels of matrix metalloproteinases (MMPs) are produced within the tissue of patients with the condition. In the present study, we sought to determine whether serum and plasma levels of MMPs and tissue inhibitors of metalloproteinases (TIMPs) are also elevated.Methods. Blood samples were obtained from 16 patients with rapidly destructive hip OA and from 20 patients with OA before total hip arthroplasty was performed. Synovial specimens were obtained during surgery. Synovial fibroblasts that had migrated sufficiently from explants were subcultured in vitro for 72 hours after confluency, and harvested supernatants were collected. Blood, tissue samples, and fibroblasts were assayed for MMPs 1, 2, 3, and 9, and TIMPs 1 and 2 by sandwich enzyme immunoassay.Results. In blood samples, the levels of MMP-3 and MMP-9 in the group with rapidly destructive hip OA were significantly higher than the normal range and were also significantly higher than those in the OA group. In tissue samples, the levels of MMP-1, MMP-3, MMP-9, and TIMP-1 in the group with rapidly destructive hip OA were significantly higher than those in the OA group. Conclusion.The results of this study show that serum and plasma levels of MMP-3 and MMP-9 are significantly increased in patients with rapidly destructive hip OA. Significantly large amounts of these MMPs produced in synovial tissues within the hip joint could contribute in part to elevation of blood levels. Detection of increased levels of MMP-3 and MMP-9 in patients with painful, disabling hip OA may be of diagnostic value for rapidly destructive hip OA.

Circulating matrix metalloproteinases and their tissue inhibitors as markers for ethnic variation in pelvic floor tissue integrity

“…KHC3081, KHC3061, KHC1491 and KHC3081, respectively; Thermo Fisher Scientific, Inc., Waltham, MA, USA). In each assay, the proactive and active forms of both MMP-2 and -9 were processed according to the manufacturer's instructions, as previously described (14,15). Prior to analysis, the samples were thawed briefly in a 37˚C water bath and placed on ice.…”

The Role of Vitamin D in Osteoarthritis and Rheumatic Disease

“…Tissue and circulating concentrations of MMPs are elevated in patients with OA [20,21]. Observational human studies showed vitamin D status to be inversely associated with circulating MMP9 in healthy adults, while vitamin D 3 supplementation (3 monthly injections of either 500 IU or 50 000 IU) reduced MMP9 concentrations in adults with vitamin D insufficiency [22].…”

Arthropathies destructrices du sujet âgé

“…Enfin, Masuhara une coxarthrose d'évolution lente [23]. Ces deux résultats reflètent la sévérité de la chondrolyse et l'intensité de l'activité protéasique dans la CDR.…”