Silenced ZNF154 Is Associated with Longer Survival in Resectable Pancreatic Cancer

Wiesmueller, Felix; Köpke, J.; Aust, Daniela E.; Roy, Janine; Dahl, Andreas; Pilarsky, Christian; Grützmann, Robert

doi:10.3390/ijms20215437

Cited by 8 publications

(13 citation statements)

References 33 publications

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“… 2 A recent study also showed the effects of ZNF154 on cell viability and gene expression in vitro. 1 In the present study, ZNF154 upregulation revealed obvious effects on the growth and viability of MGC-803 GC cells, indicating that ZNF154 silencing should be considered as a relevant factor for GC growth. ZNF154 re-expression decreased the mRNA level of HGF, a specifically proliferative gene, in MGC-803 cells, indicating a potential molecular mechanism of ZNF154-mediated inhibition of GC growth.…”

Section: Discussionsupporting

confidence: 51%

“…The zinc finger protein 154 ( ZNF154 ) gene have been reported as a tumor suppressor gene in the zinc finger protein family. 1 Compelling evidence has shown that ectopic expression of the ZNF154 gene would suppress tumor cell growth, migration, invasion, and colony formation in nasopharyngeal carcinoma. 2 Studies have shown that the count of methylated CpG sites in the ZNF154 DNA promoter was positively associated with longer survival in respectable pancreatic cancer.…”

Section: Introductionmentioning

confidence: 99%

“… 2 Studies have shown that the count of methylated CpG sites in the ZNF154 DNA promoter was positively associated with longer survival in respectable pancreatic cancer. 1 Hypermethylation of ZNF154 leads to inhibition of gene expression inhibition and loss of function, which contribute to cancer development and poor patient outcomes. 3 , 4 In addition, researchers recently revealed a novel molecular mechanism.…”

Section: Introductionmentioning

confidence: 99%

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Low Expression of ZNF154 is Related to Poor Prognosis in Gastric Cancer

He¹,

Huang²,

Tang³

et al. 2022

CMAR

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Introduction Zinc finger protein 154 ( ZNF154 ) has been identified as a tumor suppressor gene in multiple carcinomas. Lymph node (LN) metastasis is one of the most intensively negative factor of gastric cancer (GC) prognosis. However, the potential mechanisms of ZNF154-mediated LN metastasis are not elucidated. This study aimed to investigate the role of ZNF154 in LN metastasis of GC and their underlying mechanisms through in vitro and in vivo experiments. Methods Antitumor effect was measured by growth inhibition by cell counting kit-8 (CCK-8) and colony formation assay. Cell cycle distribution and apoptosis were evaluated by flow cytometry. Cell migration and invasion were measured by wound healing and transwell invasion assays, respectively. The expression levels of proteins were analyzed by Western blot. Xenograft models were used for validation in vivo. Results Our research showed that ZNF154 was down-regulated in 81.43% (57 of 70) of GC tissues compared with 58.6% of paired non-tumor tissues from patients, ZNF154 was down-regulated in 100% (7 of 7) of GC cell lines, up-regulated expression of ZNF154 in MGC-803 GC cells reduced cell proliferation, viability, migration and invasion, and enhanced cell apoptosis and arrested cell cycle in G2 phase, and suppressed tumorigenicity of MGC-803 cells in mice. Furthermore, up-regulated expression of ZNF154 mRNA reduced the expression of B-cell lymphoma-2 (Bcl-2), matrix metalloproteinase 2 (MMP-1), hepatocyte growth factor (HGF), vascular endothelial growth factor-A/C (VEGF-A/C). Conclusion ZNF154 inhibited LN metastasis of GC cells by suppressing several biological events of GC cells. ZNF154 was a tumor suppressor gene that is a promising target for blocking nodal involvement in GC.

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Section: Discussionsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Low Expression of ZNF154 is Related to Poor Prognosis in Gastric Cancer

He¹,

Huang²,

Tang³

et al. 2022

CMAR

View full text Add to dashboard Cite

show abstract

“…With the development of gene detection technology, more and more oncogenes and anti-oncogenes have been found to be involved in the occurrence of PC. For example, it was reported that silenced ZNF154 was in relation to longer survival in resectable PC [ 18 ]. Besides, SPOP suppressed PC progression by promoting the degradation of NANOG [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

ZC3HAV1 promotes the proliferation and metastasis via regulating KRAS in pancreatic cancer

Huang

Hua

Xiao

et al. 2021

Aging

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Proliferation and metastasis are important malignant features of pancreatic cancer (PC), but the underlying molecular mechanism is unclear. ZC3HAV1, a PARP family member of proteins-enzymes, has been considered to play a significant part in a variety of biological processes. Nonetheless, the functions of ZC3HAV1 in developing PC are still unknown. This research aims to explore the biological function and the expression of ZC3HAV1 shown in PC. In our study, PCR analysis suggested that ZC3HAV1 was expressed at a high level in PC tissues and cell lines, and high ZC3HAV1 expression was remarkably related to poor prognosis. The functional assays indicated that upregulated ZC3HAV1 accelerated PC cell proliferation along with colony formation capacities in vitro . Subsequently, ZC3HAV1 could upregulate cyclin D1 and CDK2 and also promote G1/S transition in cells of PC. What’s more, we also discovered that ZC3HAV1 promotes the migration and the invasion of PC cells. It upregulates the expression of EMT (epithelial-mesenchymal transition) relevant markers. Conversely, the functional assays showed that ZC3HAV1 knockdown significantly reduced tumorigenesis. Using bioinformatics analysis and immunoprecipitation assays we found that ZC3HAV1 could directly bind to KRAS and positively regulate its expression. Furthermore, ZC3HAV1 overexpression activated MAPK signaling by increasing p-ERK levels. Conversely, knockdown of KRAS attenuated ZC3HAV1-mediated promotion of proliferation and invasion in cells of PC. The result indicated that ZC3HAV1 was in relation to poor prognosis and accelerated the proliferation and metastasis of PC cells by regulation of KRAS. Our research may offer brand-new evidence to diagnose and treat PC in clinic.

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“…Among genes associated with the 38-classifier probes, hypermethylation vs hypomethylation was already reported in different tumor types (with methylation changes being in the same direction as in our study). For instance, hypomethylation of ZNF154 was associated with worsened OS in pancreatic cancer 27 while TTC28 hypomethylation, a target of TP53, was associated with genetic instability and L1-element transpositions in colorectal cancer. 28 Likewise, DCC was reportedly stably hypermethylated in HNSCC, independent of smoking status.…”

Section: Discussionmentioning

confidence: 99%

Tumor DNA‐methylome derived epigenetic fingerprint identifies HPV‐negative head and neck patients at risk for locoregional recurrence after postoperative radiochemotherapy

Tawk

Wirkner

Schwager

et al. 2021

Intl Journal of Cancer

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Biomarkers with relevance for loco-regional therapy are needed in human papillomavirus negative aka HPV(À) head and neck squamous cell carcinoma (HNSCC). Based on the premise that DNA methylation pattern is highly conserved, we sought to develop a reliable and robust methylome-based classifier identifying HPV(À) HNSCC patients at risk for loco-regional recurrence (LR) and all-event progression after postoperative radiochemotherapy (PORT-C). The training cohort consisted of HPV-DNA negative HNSCC patients (n = 128) homogeneously treated with PORT-C in frame of the German Cancer Consortium-Radiation Oncology Group (DKTK-ROG) multicenter biomarker trial. DNA Methylation analysis was performed using Illumina 450 K and 850 K-EPIC microarray technology. The performance of the classifier was integrated with a series of biomarkers studied in the training set namely hypoxia-, 5-microRNA (5-miR), stem-cell gene-expression signatures and immunohistochemistry (IHC)-based immunological characterization of tumors (CD3/CD8/PD-L1/PD1). Validation occurred in an independent cohort of HPV(À) HNSCC patients, pooled from two German centers (n = 125). We identified a 38-methylation probe-based HPV(À)

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Silenced ZNF154 Is Associated with Longer Survival in Resectable Pancreatic Cancer

Cited by 8 publications

References 33 publications

Low Expression of ZNF154 is Related to Poor Prognosis in Gastric Cancer

Low Expression of ZNF154 is Related to Poor Prognosis in Gastric Cancer

ZC3HAV1 promotes the proliferation and metastasis via regulating KRAS in pancreatic cancer

Tumor DNA‐methylome derived epigenetic fingerprint identifies HPV‐negative head and neck patients at risk for locoregional recurrence after postoperative radiochemotherapy

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