Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach

Tateishi, Kosuke; Watanabe, Takayuki; Nishino, Hiroshi; Mizunami, Makoto; Watanabe, Hidehiro

doi:10.1016/j.isci.2022.104272

Cited by 18 publications

(19 citation statements)

References 74 publications

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“…Silencing AhygOrco prolongs the time needed for A. hygrophila to detect its host, althought it does not completely disable the ability to find its host. This is consistent with Balkenius's study (Balkenius et al, 2009;Tateishi et al, 2022). These facts demonstrate that AhygOrco is a key receptor in A. hygrophila olfaction, that the OR pathway is important for insects to be able to carry out normal physiological life activities and that other sensory systems synergistic with olfaction should be further defined in the future.…”

Section: Discussionsupporting

confidence: 90%

RNAi verifications on olfactory defects of an essential biocontrol agent Agasicles hygrophila (Coleoptera: Chrysomelidae) regarding mating and host allocation

et al. 2023

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Alligator weed Alternanthera philoxeroides is a perennial, worldwide pernicious weed. The beetle Agasicles hygrophila is considered to be a classical biological agent used to control A. philoxeroides. In the insect peripheral olfactory system, the odorant receptor co-receptor (ORco) plays an important function in the perception of odors in insects. However, the function of ORco in the mating and host-finding behaviors of A. hygrophila remains unclear. In this study, we characterized the odorant receptor co-receptor of A. hygrophila (AhygOrco). Real-time quantitative PCR (qRT–PCR) showed that AhygOrco was predominantly expressed in the antennae of both male and female adults, and the difference between male and female antennae was not significant. The RNA interference (RNAi) results showed that compared to the control, the injection of AhygOrco dsRNA strongly reduced the expression of AhygOrco by 90% in male beetles and 89% in female beetles. The mate-seeking and feeding behavior of AhygOrco-silenced beetles were significantly inhibited. Male adults were significantly less successful in finding a mate compared to the control group. Furthermore, host allocation abilities toward A. philoxeroides of both adults were significantly repressed. These results indicated that AhygOrco is associated with A. hygrophila feeding and mate-seeking and that inhibition of AhygOrco expression is one of the causes of reduced host and mate recognition in A. hygrophila. Meanwhile, the study provides support for exploring gene functions based on RNAi.

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Section: Discussionsupporting

confidence: 90%

RNAi verifications on olfactory defects of an essential biocontrol agent Agasicles hygrophila (Coleoptera: Chrysomelidae) regarding mating and host allocation

et al. 2023

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“…To deliver vaporized CHCs to an antenna, we attached three heaters to our olfactory stimulation apparatus for SSR ( Tateishi et al, 2022 ). Fresh air taken from outdoors via a diaphragm pump was cleaned and dried by charcoal and silica-gel filters, respectively.…”

Section: Methodsmentioning

confidence: 99%

Cuticular hydrocarbon reception by sensory neurons in basiconic sensilla of the Japanese carpenter ant

Watanabe

Ogata

Nodomi

et al. 2023

Front. Cell. Neurosci.

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To maintain the eusociality of a colony, ants recognize subtle differences in colony-specific sets of cuticular hydrocarbons (CHCs). The CHCs are received by female-specific antennal basiconic sensilla and processed in specific brain regions. However, it is controversial whether a peripheral or central neural mechanism is mainly responsible for discrimination of CHC blends. In the Japanese carpenter ant, Camponotus japonicus, about 140 sensory neurons (SNs) are co-housed in a single basiconic sensillum and receive colony-specific blends of 18 CHCs. The complexity of this CHC sensory process makes the neural basis of peripheral nestmate recognition difficult to understand. Here, we electrophysiologically recorded responses of single basiconic sensilla to each of 18 synthesized CHCs, and identified CHC responses of each SN co-housed in a single sensillum. Each CHC activated different sets of SNs and each SN was broadly tuned to CHCs. Multiple SNs in a given sensillum fired in synchrony, and the synchronicity of spikes was impaired by treatment with a gap junction inhibitor. These results indicated that SNs in single basiconic sensilla were electrically coupled. Quantitative analysis indicated that the Japanese carpenter ants have the potential to discriminate chemical structures of CHCs based on the combinational patterns of activated SNs. SNs of ants from different colonies exhibited different CHC response spectra. In addition, ants collected from the same colony but bred in separate groups also exhibited different CHC response spectra. These results support the hypothesis that the peripheral sensory mechanism is important for discrimination between nestmate and non-nestmate ants.

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“…However, the onset of the inhibitory phase occurred significantly later than the offset of the stimulus (Figure 12E). The prolonged discharge of action potentials by ORNs in response to an odor stimulus, exceeding the duration of the stimulus, has been reported in many different species (locust (Raman et al 2010), honeybee (Getz and Akers 1993), cockroach (Tateishi et al 2022), moths (Grémiaux et al 2012, Jarriault et al 2010, Kaissling et al 1989, Rospars et al 2014, some ORN-odor combinations in Drosophila (Martelli et al 2013, Nagel andWilson 2011), and Drosophila larva (Montague et al 2011)). Cockroach ORNs can faithfully encode both the onset and offset of a stimulus by having a pair of ORNs in each sensillum, one sensitive to odor onset and another to odor offset (Burgstaller andTichy 2011, Tichy et al 2005).…”

Section: Rapid Response Termination Stems From Slow Spike Frequency A...mentioning

confidence: 99%

Stimulus duration encoding occurs early in the moth olfactory pathway

Barta

Montsempes

Demondion

et al. 2022

Preprint

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Flying insects encounter turbulent environments, where chemotaxis along a concentration gradient makes little sense. Detection of the onset and offset of discrete odor pulses is then expected to become crucial for navigation, but it is not well understood how the olfactory system encodes the offset of the odor pulse. Previous works indicated that the duration of a male moth olfactory receptor neuron’s (ORN) spike firing response to pheromone stimuli greatly exceeds the pulse duration. However, these works were based on imprecise odor delivery systems. We built an odor delivery system capable of delivering much sharper pheromone stimuli. The stimuli evoked ORN firing responses that faithfully tracked the stimulus duration, provided the stimulus lasted at least 200 ms. A transient inhibition marked the termination of such stimuli. Shorter stimuli produced a firing response exceeding the stimulus duration. The response shapes could be explained by adaptation of the ORN on only two time scales. With simulations, we showed that the observed limits in stimulus offset detection propagate to the antennal lobe and are likely to be behaviorally significant. Our results increase the understanding of the mechanisms necessary for male moths to navigate through pheromone plumes.

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Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach

Cited by 18 publications

References 74 publications

RNAi verifications on olfactory defects of an essential biocontrol agent Agasicles hygrophila (Coleoptera: Chrysomelidae) regarding mating and host allocation

RNAi verifications on olfactory defects of an essential biocontrol agent Agasicles hygrophila (Coleoptera: Chrysomelidae) regarding mating and host allocation

Cuticular hydrocarbon reception by sensory neurons in basiconic sensilla of the Japanese carpenter ant

Stimulus duration encoding occurs early in the moth olfactory pathway

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