Silica Hydride: A Separation Material Every Analyst Should Know About

Pesek, Joseph J.; Matyska, Maria T.

doi:10.3390/molecules26247505

Cited by 3 publications

(1 citation statement)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acetonitrile or methanol is usually applied as further eluents. Depending on the analytes and the composition of the mobile phases, both RP mechanisms and normal phase‐related effects come into play [29]. Although silica hydride phases are still used relatively rarely, they have already been applied to separate various polar food extracts, for example, with asparagus [2], elderberries [14] as well as wine, tuna, and parmesan cheese [30].…”

Section: Chromatographic Separation Techniquesmentioning

confidence: 99%

Food metabolomics: Latest hardware—developments for nontargeted food authenticity and food safety testing

Creydt¹,

Fischer²

2022

Electrophoresis

View full text Add to dashboard Cite

The analytical requirements for food testing have increased significantly in recent years. On the one hand, because food fraud is becoming an ever-greater challenge worldwide, and on the other hand because food safety is often difficult to monitor due to the far-reaching trade chains. In addition, the expectations of consumers on the quality of food have increased, and they are demanding extensive information. Cutting-edge analytical methods are required to meet these demands. In this context, non-targeted metabolomics strategies using mass and nuclear magnetic resonance spectrometers (mass spectrometry [MS]) have proven to be very suitable. MS-based approaches are of particular importance as they provide a comparatively high analytical coverage of the metabolome.Accordingly, the efficiency to address even challenging issues is high. A variety of hardware developments, which are explained in this review, have contributed to these advances. In addition, the potential of future developments is highlighted, some of which are currently not yet commercially available or only used to a comparatively small extent but are expected to gain in importance in the coming years.

show abstract

Section: Chromatographic Separation Techniquesmentioning

confidence: 99%

Food metabolomics: Latest hardware—developments for nontargeted food authenticity and food safety testing

Creydt¹,

Fischer²

2022

Electrophoresis

View full text Add to dashboard Cite

show abstract

A Compendium of the Principal Stationary Phases Used in Hydrophilic Interaction Chromatography: Where Have We Arrived?

et al. 2022

View full text Add to dashboard Cite

Hydrophilic interaction liquid chromatography (HILIC) today is a well-known and largely applied technique to analyse polar compounds such as pharmaceuticals, metabolites, proteins, peptides, amino acids, oligonucleotides, and carbohydrates. Due to the large number of stationary phases employed for HILIC applications, this review aims to help the reader in choosing a proper stationary phase, which often represents the critical point for the success of a separation. A great offer is present for achiral applications in contrast to the chiral phases developed for HILIC enantioseparations. In the last case, up-to-date solutions are presented.

show abstract

Validation of an Aqueous Normal Phase Chromatography Method for the Analysis of Ergothioneine in Commercial Mushrooms

Pesek,

Matyska,

Hiltz

et al. 2023

LCGC N. Am.

View full text Add to dashboard Cite

An analytical HPLC method for the identification and quantification of ergothioneine (EGT) has been developed using a silica hydride-based column. A simplified sample preparation method was developed. Several types of commercially available mushrooms were analyzed for quantification of ergothioneine using a Cogent Diamond Hydride column (100 x 4.6 mm) at a flow rate of 1 mL/min, with a deionized water:acetonitrile:0.1% formic acid mobile phase using an aqueous normal phase (ANP) gradient. EGT was detected at 254 nm and was identified by matching retention times of the verified standard. The standard ergothioneine content ranged from 0.01 mg to 0.1 mg/mL with excellent linearity correlation (R2 = 0.99999). EGT recoveries were within 96.5–100.3%. The developed HPLC method was simple, accurate, reproducible, sensitive and rugged.

show abstract

Silica Hydride: A Separation Material Every Analyst Should Know About

Cited by 3 publications

References 37 publications

Food metabolomics: Latest hardware—developments for nontargeted food authenticity and food safety testing

Food metabolomics: Latest hardware—developments for nontargeted food authenticity and food safety testing

A Compendium of the Principal Stationary Phases Used in Hydrophilic Interaction Chromatography: Where Have We Arrived?

Validation of an Aqueous Normal Phase Chromatography Method for the Analysis of Ergothioneine in Commercial Mushrooms

Contact Info

Product

Resources

About