Silica nanoparticles as an enhancer in the IL-1β-induced inflammation cycle of A549 cells

Wu, Jing; Han, Yuge; Zou, Xiaoqian; Zhu, Kui; Wang, Zichen; Ye, Xiaohong; Liu, Yumei; Dong, Shihua; Chen, Xiaojing; Liu, Dandan; Wen, Zihao; Wang, Yao; Huang, Shiqi; Zhou, Zixing; Zeng, Chengli; Huang, Chuican; Zheng, Shimin; Du, Xiuben; Huang, Xueling; Zhang, Baohuan; Jing, Chunxia; Yang, Guang

doi:10.1080/08923973.2019.1569046

Cited by 22 publications

(13 citation statements)

References 24 publications

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“…The RHS-induced IL-1β, IL-6, IL-15, and TNF-α cytokine and COX-2a expression in zebrafish suggests that RHS could enhance immunity against bacterial infection. Studies have reported that exposure to silica nanoparticles induced cytokine (IL-1β, IL-6, and TNF-α) expression in cell lines, mice, and zebrafish, also supporting the results of the present study, although the subjects of these studies focus on the toxicity of silica nanoparticles [45][46][47]. Lysozyme is an indispensable enzyme that functions as a humoral component of the innate immune defense system in resisting pathogen infections by hydrolyzing the peptidoglycan of bacterial cell walls.…”

Section: Discussionsupporting

confidence: 89%

Rice Husk Silica Enhances Innate Immune in Zebrafish (Danio rerio) and Improves Resistance to Aeromonas hydrophila and Streptococcus iniae Infection

et al. 2019

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Rice husk (RH) contains abundant silica such that RH silica (RHS) may be useful for possible industrial exploitation. Here, amorphous silica nanoparticles with multiple pore structures were acquired from RH by simple thermochemical processes. RHS antimicrobial activity and effects on zebrafish innate immunity against pathogen infections were evaluated. A toxicity assay showed that zebrafish exposed to an RHS dose lower than 200 μg/mL did not exhibit damage to zebrafish embryonic development or juvenile survival. RHS showed a wide spectrum of bacteriostatic activity against a variety of pathogens including antibiotic-resistant pathogens, implying its potential application as an antimicrobial agent in diverse industries. Fish exposed to 20 or 200 μg/mL RHS exhibited significantly increased mRNA expression of immune-related genes, including IL-1β, IL-6, IL-15, TNF-α, COX-2a, TLR-4a, lysozyme, and complement C3b. RHS-treated zebrafish exhibited a higher cumulative survival compared to that in control fish after infecting with Aeromonas hydrophila and Streptococcus iniae. The present results showed that a safe RHS dose enhanced innate immunity against infections without toxic effects in healthy fish, suggesting that RHS may be developed as an immunostimulant for improving health status in aquaculture.

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Section: Discussionsupporting

confidence: 89%

Rice Husk Silica Enhances Innate Immune in Zebrafish (Danio rerio) and Improves Resistance to Aeromonas hydrophila and Streptococcus iniae Infection

et al. 2019

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“…In contrast, both cell systems revealed an increased IL-8 expression, in contrast to the observation of Wand and colleagues, who observed a cell-system-specific IL-8 response [ 26 ]. All of these genes are cellular markers for a pro-inflammatory response [ 52 , 53 , 54 ]; therefore, the up-regulation of these genes indicates an inflammatory potential of CuO NP. However, the dose-dependent down-regulation of CCL22 and IL-1b does not match this scenario.…”

Section: Discussionmentioning

confidence: 99%

Impact of Differentiated Macrophage-Like Cells on the Transcriptional Toxicity Profile of CuO Nanoparticles in Co-Cultured Lung Epithelial Cells

Hufnagel

Neuberger

Wall

et al. 2021

IJMS

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To mimic more realistic lung tissue conditions, co-cultures of epithelial and immune cells are one comparatively easy-to-use option. To reveal the impact of immune cells on the mode of action (MoA) of CuO nanoparticles (NP) on epithelial cells, A549 cells as a model for epithelial cells have been cultured with or without differentiated THP-1 cells, as a model for macrophages. After 24 h of submerged incubation, cytotoxicity and transcriptional toxicity profiles were obtained and compared between the cell culture systems. Dose-dependent cytotoxicity was apparent starting from 8.0 µg/cm2 CuO NP. With regard to gene expression profiles, no differences between the cell models were observed concerning metal homeostasis, oxidative stress, and DNA damage, confirming the known MoA of CuO NP, i.e., endocytotic particle uptake, intracellular particle dissolution within lysosomes with subsequent metal ion deliberation, increased oxidative stress, and genotoxicity. However, applying a co-culture of epithelial and macrophage-like cells, CuO NP additionally provoked a pro-inflammatory response involving NLRP3 inflammasome and pro-inflammatory transcription factor activation. This study demonstrates that the application of this easy-to-use advanced in vitro model is able to extend the detection of cellular effects provoked by nanomaterials by an immunological response and emphasizes the use of such models to address a more comprehensive MoA.

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“…The results of other studies are compatible with the results of the present study, associating the increase in the toxic effects with an increase in the concentration of A-SiO 2 (Ahamed, 2013; Lee et al, 2013) and iron oxide nanoparticles (Helmig et al, 2018; Khan et al, 2012). Wu et al (2019) stated that A-SiO 2 nanoparticles induce the cytokine compounds such as interleukin-6 and promote the A 549 cells’ inflammatory response and cytotoxicity. Some authors in respect to iron nanoparticle exposure stated that iron overload in cells could initiate Fenton’s reaction and production of ROS, followed by a change in the MMP and cellular damage (Kai et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Interactive toxicity effect of combined exposure to hematite and amorphous silicon dioxide nanoparticles in human A₅₄₉ cell line

et al. 2021

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The study on the health effects of combined exposure to various contaminants has been recommended by many authors. The objective of the present study was to examine the effects of the co-exposure to hematite and amorphous silicon dioxide (A-SiO2) nanoparticles on the human lung A549 cell line. The A549 cell line was exposed to 10, 50, 100, and 250 µg/ml concentrations of hematite and A-SiO2 nanoparticles both independently and in combination. Their toxicity in both circumstances was investigated by MTT, intracellular reactive oxygen species, cell glutathione content, and mitochondrial membrane potential tests, and the type of interaction was investigated by statistical analysis using Statistical Package for Social Sciences (SPSS, v. 21). Results showed that the independent exposure to either hematite or A-SiO2 compared with the control group produced more toxic effects on the A549 cell line. The toxicity of combined exposure of the nanoparticles was lower compared with independent exposure, and antagonistic interactive effects were detected. The findings of this study could be useful in clarifying the present debate on the health effects of combined exposure of hematite and A-SiO2 nanoparticles. Because of the complexities of combined exposures, further studies of this kind are recommended.

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Silica nanoparticles as an enhancer in the IL-1β-induced inflammation cycle of A549 cells

Cited by 22 publications

References 24 publications

Rice Husk Silica Enhances Innate Immune in Zebrafish (Danio rerio) and Improves Resistance to Aeromonas hydrophila and Streptococcus iniae Infection

Rice Husk Silica Enhances Innate Immune in Zebrafish (Danio rerio) and Improves Resistance to Aeromonas hydrophila and Streptococcus iniae Infection

Impact of Differentiated Macrophage-Like Cells on the Transcriptional Toxicity Profile of CuO Nanoparticles in Co-Cultured Lung Epithelial Cells

Interactive toxicity effect of combined exposure to hematite and amorphous silicon dioxide nanoparticles in human A₅₄₉ cell line

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Silica nanoparticles as an enhancer in the IL-1β-induced inflammation cycle of A549 cells

Cited by 22 publications

References 24 publications

Rice Husk Silica Enhances Innate Immune in Zebrafish (Danio rerio) and Improves Resistance to Aeromonas hydrophila and Streptococcus iniae Infection

Rice Husk Silica Enhances Innate Immune in Zebrafish (Danio rerio) and Improves Resistance to Aeromonas hydrophila and Streptococcus iniae Infection

Impact of Differentiated Macrophage-Like Cells on the Transcriptional Toxicity Profile of CuO Nanoparticles in Co-Cultured Lung Epithelial Cells

Interactive toxicity effect of combined exposure to hematite and amorphous silicon dioxide nanoparticles in human A549 cell line

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Interactive toxicity effect of combined exposure to hematite and amorphous silicon dioxide nanoparticles in human A₅₄₉ cell line