Silk-based hybrid microfibrous mats as guided bone regeneration membranes

Wu, Mi; Han, Zhengyi; Li, Wen; Yao, Jinrong; Zhao, Biwei; Shao, Zhengzhong; Chen, Xin

doi:10.1039/d0tb02687e

Cited by 31 publications

(27 citation statements)

References 51 publications

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“…Laponite (Lap, Na 0.7 + [(Mg 5.5 Li 0.3 )Si 8 O 20 (OH) 4 ] 0.7 - ) is one of the clays that can be used to produce these hydrogels [ 8 , 9 ]. This nanoclay is a synthetic smectic with 25–30 nm diameter of and 1 nm thickness [ 10 ]. Lap crystals have positive charges on the edge and negative charges on the top and bottom surfaces [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Nanocomposite Hydrogel Produced from PEGDA and Laponite for Bone Regeneration

Magalhães

Andrade

Bezerra

et al. 2022

JFB

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Herein, a nanocomposite hydrogel was produced using laponite and polyethylene-glycol diacrylate (PEGDA), with or without Irgacure (IG), for application in bone tissue regeneration. The nanocomposites were characterized by X-ray diffraction (XRD), Fourier-Transform infrared spectroscopy (FTIR), and thermal analysis (TG/DTG). The XRD results showed that the crystallographic structure of laponite was preserved in the nanocomposite hydrogels after the incorporation of PEGDA and IG. The FTIR results indicated that PEGDA polymer chains were entangled on laponite in hydrogels. The TG/DTG found that the presence of laponite (Lap) improved the thermal stability of nanocomposite hydrogel. The toxicity tests by Artemia salina indicated that the nanocomposite hydrogels were not toxic, because the amount of live nauplii was 80.0%. In addition, in vivo tests demonstrated that the hydrogels had the ability to regenerate bone in a bone defect model of the tibiae of osteopenic rats. For the nanocomposite hydrogel (PEGDA + Lap nanocomposites + UV light), the formation of intramembranous bone in the soft callus was more intense in 66.7% of the animals. Thus, the results presented in this study evidence that nanocomposite hydrogels obtained from laponite and PEGDA have the potential for use in bone regeneration.

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Section: Introductionmentioning

confidence: 99%

Nanocomposite Hydrogel Produced from PEGDA and Laponite for Bone Regeneration

Magalhães

Andrade

Bezerra

et al. 2022

JFB

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“…Functionality for biomedicine has been one of the research focuses for SF. Recently, the literature aimed to investigate SF’s cell adhesion, drug-loading capacity, and osteoinductivity [ 131 , 132 , 133 , 134 , 135 ]. Some materials are usually applied to composite SF in hard tissue engineering to improve the biological properties.…”

Section: Bonementioning

confidence: 99%

A Review of Recent Advances in Natural Polymer-Based Scaffolds for Musculoskeletal Tissue Engineering

et al. 2022

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The musculoskeletal (MS) system consists of bone, cartilage, tendon, ligament, and skeletal muscle, which forms the basic framework of the human body. This system plays a vital role in appropriate body functions, including movement, the protection of internal organs, support, hematopoiesis, and postural stability. Therefore, it is understandable that the damage or loss of MS tissues significantly reduces the quality of life and limits mobility. Tissue engineering and its applications in the healthcare industry have been rapidly growing over the past few decades. Tissue engineering has made significant contributions toward developing new therapeutic strategies for the treatment of MS defects and relevant disease. Among various biomaterials used for tissue engineering, natural polymers offer superior properties that promote optimal cell interaction and desired biological function. Natural polymers have similarity with the native ECM, including enzymatic degradation, bio-resorb and non-toxic degradation products, ability to conjugate with various agents, and high chemical versatility, biocompatibility, and bioactivity that promote optimal cell interaction and desired biological functions. This review summarizes recent advances in applying natural-based scaffolds for musculoskeletal tissue engineering.

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“…Bai et al have prepared hydrogels with robust mechanical properties through the combination of dynamic host‐guest interactions in silk hydrogels 19 . Moreover, some researchers have used electrospinning or 3D printing to prepare scaffolds with high porosity for silk fibroin 20–23 . However, the hydrogel lacks the anisotropic microstructure of natural tissues, sometimes it is difficult to provide the physical guidance signals required by tissue cells and the mechanical properties of freeze‐dried pure silk fibroin scaffolds are usually unsatisfactory.…”

Section: Introductionmentioning

confidence: 99%

“…19 Moreover, some researchers have used electrospinning or 3D printing to prepare scaffolds with high porosity for silk fibroin. [20][21][22][23] However, the hydrogel lacks the anisotropic microstructure of natural tissues, sometimes it is difficult to provide the physical guidance signals required by tissue cells and the mechanical properties of freeze-dried pure silk fibroin scaffolds are usually unsatisfactory. Polyurethane (PU), a traditional chemical product, has been famous for its chemical stability and mechanical properties such as excellent high strength, elasticity and toughness, which can make up for the lack of mechanical properties of silk fibroin.…”

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confidence: 99%

A novel high mechanical and excellent hydrophilic electrospun polyurethane‐silk‐bioactive glass nanofiber film for rotator cuff injury repair

Duan

Ling

Sun

et al. 2021

J of Applied Polymer Sci

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Rotator cuff injury is the most common muscle injury in bone surgery and there was still enormously challenging to completely repair it. Disturbed by mechanical stability and biocompatibility, most implants have failed to relieve symptoms and prevent the development of osteoarthritis. Herein, we have firstly fabricated novel electrospinned nanofiber membranes by integrating the excellent mechanical properties of polyurethane (PU), the good biocompatibility of silk fibroin with the osteogenesis properties of bioactive glass (PSB). The obtained results have exhibited that the PSB nanofiber film possessed excellent mechanical properties with the tensile stress have reached 14.6 MPa and the tensile strain reached 70%, which had extremely remedied the properties of pure silk film (3.8 MPa and the tensile strain 7.9%). Especially, PSB has shown superior hydrophilicity (WCA) than silk (WCA) and PU (WCA) groups.Furthermore, we have endowed the PSB with osteogenesis by doping bioactive glass (BG) in it. The PSB film can long-term release functional ions (Si and B ions) that benefit bone tissue repair, and the CCK-8 and ALP results have also confirmed the excellent biocompatibility and osteogenesis of PSB. Hence, the novel PSB film may provide a new treatment strategy for repairing rotator cuff injury.

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Silk-based hybrid microfibrous mats as guided bone regeneration membranes

Abstract: LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

Cited by 31 publications

References 51 publications

Nanocomposite Hydrogel Produced from PEGDA and Laponite for Bone Regeneration

Nanocomposite Hydrogel Produced from PEGDA and Laponite for Bone Regeneration

A Review of Recent Advances in Natural Polymer-Based Scaffolds for Musculoskeletal Tissue Engineering

A novel high mechanical and excellent hydrophilic electrospun polyurethane‐silk‐bioactive glass nanofiber film for rotator cuff injury repair

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