Silk-Based Scaffold for Ligament Tissue Engineering

Liu, H.; Fan, Hongbin; Wong, Eugene J.W.; Toh, Siew-Lok; Goh, James Cho‐Hong

doi:10.1007/978-3-540-69367-3_10

Cited by 6 publications

(1 citation statement)

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“…[70][71][72][73] Tenomodulin and tenascinc are biomarkers of early stage ligament repair and are necessary for cell proliferation and collagen fibril maturation. [74][75][76] Collagen type I is a more mature form of collagen that appears later in the ligamentogenesis process, 77 and fibronectin is a biomarker of early stage ligament repair that is abundantly expressed during the proliferation phase of ligamentogenesis. [78][79][80] High expression of decorin and tenomodulin and low expression of Col1 and collagen type III in native CrCL tissue are consistent with mature tissue.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of canine adipose–derived multipotent stromal cell differentiation to ligamentoblasts on tensioned collagen type I templates in a custom bioreactor culture system

Taguchi

Zhang

Angibeau

et al. 2021

ajvr

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OBJECTIVE To evaluate differentiation of canine adipose–derived multipotent stromal cells (ASCs) into ligamentoblasts on tensioned collagen type I (Col1) templates in a perfusion culture system. SAMPLES Infrapatellar fat pad ASCs from healthy stifle joints of 6 female mixed-breed dogs. PROCEDURES Third-passage ASCs (6 × 106 cells/template) were loaded onto suture-augmented Col1 templates under 15% static strain in perfusion bioreactors. Forty-eight ASC-Col1 constructs were incubated with ligamentogenic (ligamentogenic constructs; n = 24) or stromal medium (stromal constructs; 24) for up to 21 days. Specimens were collected from each construct after 2 hours (day 0) and 7, 14, and 21 days of culture. Cell number, viability, distribution, and morphology; construct collagen content; culture medium procollagen-I-N-terminal peptide concentration; and gene expression were compared between ligamentogenic and stromal constructs. RESULTS ASCs adhered to collagen fibers. Cell numbers increased from days 0 to 7 and days 14 to 21 for both construct types. Relative to stromal constructs, cell morphology and extracellular matrix were more mature and collagen content on day 21 and procollagen-I-N-terminal peptide concentration on days 7 and 21 were greater for ligamentogenic constructs. Ligamentogenic constructs had increased expression of the genes biglycan on day 7, decorin throughout the culture period, and Col1, tenomodulin, fibronectin, and tenascin-c on day 21; expression of Col1, tenomodulin, and tenascin-c increased between days 7 and 21. CONCLUSIONS AND CLINICAL RELEVANCE Ligamentogenic medium was superior to stromal medium for differentiation of ASCs to ligamentoblasts on suture-augmented Col1 scaffolds. Customized ligament neotissue may augment treatment options for dogs with cranial cruciate ligament rupture.

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