Silver - calcium titanate – titania decorated Ti6Al4V powders: An antimicrobial and biocompatible filler in composite scaffold for bone tissue engineering application

Venkatesan, K.; Mathew, Ann; Sreya, P V; Raveendran, Subina; Rajendran, A.; Subramanian, B.; Pattanayak, Deepak K.

doi:10.1016/j.apt.2021.10.008

Cited by 8 publications

(13 citation statements)

References 46 publications

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“…The individual photoemission spectra of each element were plotted concerning the C 1s peak position at 284.8 eV . The XPS spectrum of Ti 2p displays two peaks centered at 463.92 and 458.02 eV (Figure b) corresponding to the typical binding energies of Ti 2p 1/2 and Ti 2p 3/2 in TiO 2 , respectively . The peaks located around 529.6 and 531.5 eV were assigned to O 1s (Figure c) with respect to the previous reports and correspond to lattice oxygen and surface active oxygen, respectively .…”

Section: Results and Discussionmentioning

confidence: 78%

“…NaOH treatment of CP-Ti will enable the Na and O ions to penetrate into the surface to a depth of 1 μm and leads to the formation of sodium hydrogen titanate. Upon heat treatment, the surface oxide layer adheres strongly to the inner core titanium due to the formation of sodium titanate and rutile TiO 2 on the surface by the elimination of hydrogen as a water molecule . The samples of lower concentrations of Ce (TiNa0.01Ce and TiNa0.001Ce) show the presence of anatase TiO 2 over the surface.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Surface modification techniques adopted for metals and alloys can be mechanical (grinding, polishing, blasting), chemical (alkali/acid treatment, anodization), or physical (spraying, PVD). ,, These surface modification approaches are mainly aimed at making the implant surface more rough and porous. Enhanced surface roughness as well as the porous nature of the implant will lead to enriched protein adsorption that promotes better cell adhesion and their ingrowth into the porous layers of the surface. , A bioactive surface with a porous nanonetwork structure can be generated onto a Ti/Ti alloy-based implant by a simple alkaline treatment with a high concentration of NaOH. − It is also reported that elements with antimicrobial properties (Ag, Zn, Ce, etc. ), as well as enhancement of osseointegration (Ca, Mg, Zr, Se, Ce etc.…”

Section: Introductionmentioning

confidence: 99%

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Cerium Ion-Incorporated Titanium Metal Implants of Enhanced Bioactivity for Biomedical Applications

Mathew

Venkatesan

et al. 2023

ACS Appl. Bio Mater.

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The enhancement in the performance of metallic bone implants based on commercially pure titanium (CP-Ti) by incorporation of cerium (Ce) ions onto the surface was evaluated. The incorporation of Ce ions onto the CP-Ti surface was carried out by a simple two-step chemical treatment method, where an initial NaOH treatment and then a subsequent treatment with different molar concentrations of ceric nitrate solution followed by heat treatment at 600 °C were carried out. The modified surfaces were observed using field emission scanning electron microscopy (FE-SEM), scanning electron microscopy−energy dispersive X-ray analysis (SEM-EDX), X-ray photoelectron spectroscopy (XPS), the laser Raman spectroscopic technique, high-resolution transmission electron microscopy (HR-TEM), and atomic force microscopy (AFM). The formation of a nanonetwork structure by the initial NaOH treatment and the replacement of Na ions with Ce ions along with different phases of TiO 2 was evident from the surface characterization results. The transition of rutile TiO 2 to anatase TiO 2 in the modified surface is evident from the Raman spectra with respect to the treatment of higher to lower concentrations of ceric nitrate solution. The presence of two different oxidation states of Ce (Ce 3+ and Ce 4+ ) and improvement in the surface wettability were also distinct in the modified samples. Thus, the incorporated Ce ions over the nanostructured titania network showed low cytotoxicity, good cell adhesion, and enhanced extracellular mineralization on MG-63 cells with better protein adsorption in BSA medium. Taken together, the thus-improved nanostructured surface morphology with the anatase TiO 2 phase and distinct extracellular mineralization in the Ce-incorporated Ti metal with good biocompatibility make it a promising candidate for bone implant applications.

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Section: Results and Discussionmentioning

confidence: 78%

Section: Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cerium Ion-Incorporated Titanium Metal Implants of Enhanced Bioactivity for Biomedical Applications

Mathew

Venkatesan

et al. 2023

ACS Appl. Bio Mater.

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Section: Discussionmentioning

confidence: 99%

“…Recent discoveries of the antioxidant and microbial activities of CaTiO 3 -NPs containing compounds together with a high capacity of CaTiO 3 to deliver calcium ions raise interest in the use of CaTiO 3 -NPs in a wide range of medical applications [3,18]. However, the cytotoxic and genotoxic effects of CaTiO 3 -NPs in human MCF-7 cancer Although, the size of many nanoparticles measured by DLS is actually found to be larger than the size measured by TEM, there is no fixed rule for nanoparticles because the measured size of nanoparticles depends on the concentration of nanoparticles, ions and other variables.…”

Section: Discussionmentioning

confidence: 99%

Induction of oxidative DNA damage, cell cycle arrest and p53 mediated apoptosis by calcium titanate nanoparticles in MCF-7 breast cancer cells

2022

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Background The distinctive properties and high activity of calcium titanate nanoparticles (CaTiO3-NPs) increase their use in many products. However, the cytotoxic and genotoxic effects of CaTiO3-NPs in human cancer cell lines have not been well studied. Therefore, this study was conducted to explore CaTiO3-NPs induced cytotoxicity, genomic instability and apoptosis in human breast cancer (MCF-7) cells. Methods Sulforhodamine B (SRB) and the alkaline comet assays were done to study cell viability and DNA damage induction, respectively. Apoptosis induction and cell cycle distribution were analyzed using flow cytometry. The level of intracellular reactive oxygen species (ROS) was studied, and the expression levels of p53, Bax and Bcl2 genes were also measured. Results The results of the Sulforhodamine B (SRB) cytotoxicity assay showed that viability of MCF-7 cells was not affected by CaTiO3-NPs treatment for 24 h, however, exposure to CaTiO3-NPs for 72 h caused concentrations dependent death of MCF-7 cells. Treatment with CaTiO3-NPs for 72 h caused marked increases in intracellular ROS level and induced DNA damage. Treatment of MCF-7 cells with CaTiO3-NPs also caused MCF-7 cell cycle arrest at the G0 and S phases and s triggered apoptosis of MCF-7 cells by causing simultaneous increases in the expression levels of apoptotic p53 and Bax genes and a decrease in the expression level of anti-apoptotic Bcl2 gene. Conclusion Collectively, it was concluded that CaTiO3-NPs cause time- and concentration-dependent cytotoxic effects in human MCF-7 cells through induction of ROS generation, genomic instability and apoptosis. Thus it is recommended that further in vitro and in vivo studies are therefore recommended to understand the cytotoxic and biological effects of CaTiO3-NPs.

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Advancing strategies towards the development of tissue engineering scaffolds: a review

2023

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Silver - calcium titanate – titania decorated Ti6Al4V powders: An antimicrobial and biocompatible filler in composite scaffold for bone tissue engineering application

Cited by 8 publications

References 46 publications

Cerium Ion-Incorporated Titanium Metal Implants of Enhanced Bioactivity for Biomedical Applications

Cerium Ion-Incorporated Titanium Metal Implants of Enhanced Bioactivity for Biomedical Applications

Induction of oxidative DNA damage, cell cycle arrest and p53 mediated apoptosis by calcium titanate nanoparticles in MCF-7 breast cancer cells

Advancing strategies towards the development of tissue engineering scaffolds: a review

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