Silver colloidal nanoparticles: antifungal effect against adhered cells and biofilms ofCandida albicansandCandida glabrata

Abstract

The aim of this study was to evaluate the effect of silver nanoparticles (SN) against Candida albicans and Candida glabrata adhered cells and biofilms. SN (average diameter 5 nm) were synthesized by silver nitrate reduction with sodium citrate and stabilized with ammonia. Minimal inhibitory concentration (MIC) tests were performed for C. albicans (n = 2) and C. glabrata (n = 2) grown in suspension following the Clinical Laboratory Standards Institute microbroth dilution method. SN were applied to adhered cells (2 h) or biofilms (48 h) and after 24 h of contact their effect was assessed by enumeration of colony forming units (CFUs) and quantification of total biomass (by crystal violet staining). The MIC results showed that SN were fungicidal against all strains tested at very low concentrations (0.4-3.3 μg ml(-1)). Furthermore, SN were more effective in reducing biofilm biomass when applied to adhered cells (2 h) than to pre-formed biofilms (48 h), with the exception of C. glabrata ATCC, which in both cases showed a reduction ∼90%. Regarding cell viability, SN were highly effective on adhered C. glabrata and respective biofilms. On C. albicans the effect was not so evident but there was also a reduction in the number of viable biofilm cells. In summary, SN may have the potential to be an effective alternative to conventional antifungal agents for future therapies in Candida-associated denture stomatitis.

Inhibitory effect of silver nanoparticles mediated by atmospheric pressure air cold plasma jet against dermatophyte fungi

“…Even with the same species, the MIC (mg ml for griseofulvin and 40 mg ml 21 for fluconazole. The variation in AgNP toxicity level found for a fungal species in this research compared with the same species investigated by other authors may be related to the difference in susceptibility pattern of the strains or may be explained by differences in the nanoparticle synthesis method and subsequent stabilization (Monteiro et al, 2011).…”

Antimicrobial, Antimycobacterial and Antibiofi lm Properties of Couroupita guianensis Aubl. Fruit Extract

“…C. albicans bio�lms exhibited increased resistance in comparison to C. glabrata with silver nanoparticle treatment, and an 85% reduction of adherent cell growth was observed at concentration >6.7 g/mL. No effect on mature bio�lms was reported [93].…”

Biocidal potential of biosynthesized silver nanoparticles against fungal threats

“…The apoptosis in C. albicans by silver nanoparticles takes place through phosphatidylserine exposure and DNA damage followed by the activation of metacaspases. The mitochondrial integrity get disrupts thereby inducing the release of Cytochrome-C and programmed cell death occurs through ROS accumulation particularly hydroxyl free radical [26][27][28].…”