Silver(I) complexes containing diclofenac and niflumic acid induce apoptosis in human-derived cancer cell lines

Altay, Ahmet; Çağlar, Sema; Çağlar, Bülent

doi:10.1080/13813455.2019.1662454

Cited by 34 publications

(21 citation statements)

References 58 publications

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“…Taken together, our observation supports cytotoxic and anticancer properties of silver nitrate and its metronidazole and 4-hydroxymethylpyridine complexes against various cancer cells, including pancreatic cancer cells. The molecular background of this anticancer action is suggested to be associated with the induction of apoptosis [10,[22][23][24]. The results obtained in this study also support this hypothesis, since we demonstrated that silver nitrate and its complexes with metronidazole and 4-hydroxymethylpyridine induced a dose-dependent apoptotic death of PANC-1 and 1.2B4 cells.…”

Section: Discussionsupporting

confidence: 83%

Light Stability, Pro-Apoptotic and Genotoxic Properties of Silver (I) Complexes of Metronidazole and 4-Hydroxymethylpyridine against Pancreatic Cancer Cells In Vitro

Żyro

Śliwińska

Szymczak-Pajor

et al. 2020

Cancers

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Antimicrobial properties of silver (I) ion and its complexes are well recognized. However, recent studies suggest that both silver (I) ion and its complexes possess anticancer activity associated with oxidative stress-induced apoptosis of various cancer cells. In this study, we aimed to investigate whether silver nitrate and its complexes with metronidazole and 4-hydroxymethylpyridine exert anticancer action against human pancreatic cancer cell lines (PANC-1 and 1.2B4). In the study, we compared decomposition speed for silver complexes under the influence of daylight and UV-A (ultraviolet-A) rays. We employed the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazonium bromide) assay to evaluate the cytotoxicity and the alkaline comet assay to determine genotoxicity of silver nitrate and its complexes. Flow cytometry and the Annexin V-FITC/PI apoptosis detection kit were used to detect the apoptosis of human pancreatic cancer cells. We found a dose dependent decrease of both pancreatic cancer cell line viability after exposure to silver nitrate and its complexes. The flow cytometry analysis confirmed that cell death occurred mainly via apoptosis. We also documented that the studied compounds induced DNA damage. Metronidazole and 4-hydroxymethylpyridine alone did not significantly affect viability and level of DNA damage of pancreatic cancer cell lines. Complex compounds showed better stability than AgNO3, which decomposed slower than when exposed to light. UV-A significantly influences the speed of silver salt decomposition reaction. To conclude, obtained data demonstrated that silver nitrate and its complexes exerted anticancer action against human pancreatic cancer cells.

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Section: Discussionsupporting

confidence: 83%

Light Stability, Pro-Apoptotic and Genotoxic Properties of Silver (I) Complexes of Metronidazole and 4-Hydroxymethylpyridine against Pancreatic Cancer Cells In Vitro

Żyro

Śliwińska

Szymczak-Pajor

et al. 2020

Cancers

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“…Cell viability tests were performed using 2,3-bis(2methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. [56] Briefly, 100 μL of cell suspension (5x10 3 cells/well) was added into 96-well culture plates and incubated overnight in the CO 2 incubator. The next day, the cells were exposed to the different concentrations (5-250 μg/mL) of each extract (EAE, ME, and WE) and incubated for 24 h in the CO 2 incubator.…”

Section: Cell Viability Assaymentioning

confidence: 99%

A Biochemical Approach for Hedysarum candidissimum from Turkey: Screening Phytochemicals, Evaluation of Biological Activites, and Molecular Docking Study

Altay

Yeniçeri

Taslimi

et al. 2022

Chemistry & Biodiversity

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This study was designed to screen the phytochemical composition and investigate the biological activities of Hedysarum candidissimum extracts and also support the results with molecular docking studies. LC/MS/MS analysis revealed the presence of 22 phytochemical constituents (mainly phenolic acids, flavonoids, and flavonoid glycosides) in the plant structure. The methanol extract exhibited the strongest antioxidant activity among all the extracts with its strong DPPH radical scavenging and iron reducing capacity, as well as high phenolic and flavonoid contents. Additionally, it was found to be the most promising acetylcholinesterase (AChE: IC 50 : 93.26 μg/mL) and α-glycosidase (AG: IC 50 : 28.57 μg/mL) inhibitory activities, supported by the major phenolics of the species through in silico studies. Ethyl acetate extract had the strongest cytotoxic effect on HT-29 (IC 50 : 63.03 μg/mL) and MDA-MB-453 (IC 50 : 95.36 μg/mL) cancer cell lines. Both extracts exhibited considerable apoptotic and anti-migrative effects on HT-29 cells. The investigations provide phyto-analytical and biopharmacological results which can be extended by in vivo studies in the future.

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“…Numerous studies have demonstrated that metal-NSAID complexes, including zinc(II)-NSAIDs, − iridium(III)-aspirin, ruthenium(II)-NSAIDs, − nickel(II)-NSAIDs, silver(I)-NSAIDs, − manganese(II)-NSAIDs, , and organotin(IV)-NSAIDs, also exhibit potent cytotoxic activity against several cancer cells. The most likely modes of action of these complexes are increase of COX-2 selectivity, , induction of ROS, interaction with serum albumins, and intercalation of DNA .…”

Section: Development Of Nsaid Analogs and Their Applications In Cance...mentioning

confidence: 99%

NSAIDs: Old Acquaintance in the Pipeline for Cancer Treatment and Prevention─Structural Modulation, Mechanisms of Action, and Bright Future

et al. 2021

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The limitations of current chemotherapeutic drugs are still a major issue in cancer treatment. Thus, targeted multimodal therapeutic approaches need to be strategically developed to successfully control tumor growth and prevent metastatic burden. Inflammation has long been recognized as a hallmark of cancer and plays a key role in the tumorigenesis and progression of the disease. Several epidemiological, clinical, and preclinical studies have shown that traditional nonsteroidal anti-inflammatory drugs (NSAIDs) exhibit anticancer activities. This Perspective reports the most recent outcomes for the treatment and prevention of different types of cancers for several NSAIDs alone or in combination with current chemotherapeutic drugs. Furthermore, an extensive review of the most promising structural modifications is reported, such as phospho, H2S, and NO releasing-, selenium-, metal complex-, and natural product-NSAIDs, among others. We also provide a perspective about the new strategies used to obtain more efficient NSAID- or NSAID derivative- formulations for targeted delivery.

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Silver(I) complexes containing diclofenac and niflumic acid induce apoptosis in human-derived cancer cell lines

Cited by 34 publications

References 58 publications

Light Stability, Pro-Apoptotic and Genotoxic Properties of Silver (I) Complexes of Metronidazole and 4-Hydroxymethylpyridine against Pancreatic Cancer Cells In Vitro

Light Stability, Pro-Apoptotic and Genotoxic Properties of Silver (I) Complexes of Metronidazole and 4-Hydroxymethylpyridine against Pancreatic Cancer Cells In Vitro

A Biochemical Approach for Hedysarum candidissimum from Turkey: Screening Phytochemicals, Evaluation of Biological Activites, and Molecular Docking Study

NSAIDs: Old Acquaintance in the Pipeline for Cancer Treatment and Prevention─Structural Modulation, Mechanisms of Action, and Bright Future

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