Silver metal induced surface enhanced Raman of bacteria

Genetically Engineered Microbial Fuel Cells

“…Zeiri et al reported the Raman spectra of bacteria induced by silver metal. The bacteria were treated with sodium borohydride, which served as a nucleating substrate for the reduction of silver ions, and a rough silver coating formed around the microorganisms [30]. A variety of bacteria have been used to induce architectured nanostructures made of metal nanoparticles [31 -33].…”

Activation of Nanoparticles by Biosorption for E. coli Detection in Milk and Apple Juice

“…No further purification or separation was necessary due to excellent monodispersity of the nanostructures. For producing nanorice with an external silver nanoparticles coating [23,24], the suspension was washed (pelleted, rinsed with deionized water, and re-pelleted, 30 min at ∼3,400×g), suspended in 0.1 M aqueous sodium borohydride, washed again (with the second spin done for 10 min at ∼2,800×g), and then resuspended in a 0.05 M silver nitrate aqueous solution.…”

Chemotaxonomic Identification of Single Bacteria by Micro-Raman Spectroscopy: Application to Clean-Room-Relevant Biological Contaminations

“…In particular, when only a small sample amount is available, a special Raman technique called SERS spectroscopy (surface-enhanced Raman scattering spectroscopy) is especially suited. For an investigation of bacteria, various different SERS substrates or SERS microchips in combination with antibodies were tested (14,15,19,24,25,53,54,63). By applying UV-resonance Raman spectroscopy, direct investigation of macromolecules such as proteins or DNA becomes possible.…”