Silver nanoparticle applications and human health

Ahamed, Maqusood; AlSalhi, Mohamad S.; Siddiqui, M.K.J.

doi:10.1016/j.cca.2010.08.016

Cited by 1,156 publications

(809 citation statements)

References 74 publications

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“…(This is partly because the solubility of nanosilver is not fully understood, and the solubility influences the bioavailability which, in turn, influences the sensitivity of silver-resistant and silver-sensitive bacteria 17 .) Finally, we predict that the SCENIHR will underline the importance of silver speciation as this has been a general theme in many previous reviews 3,11,14,16,[18][19][20][24][25][26] .…”

mentioning

confidence: 87%

“…A further eight review articles in scientific journals followed in 2010 [11][12][13][14][15][16][17][18] , plus substantial reports commissioned by the Environmental Protection Agency (EPA) in the US (which ran to 221 pages) 19 and the Joint Research Centre (JRC) of the European Commission (426 pages) 20 . Two of the review articles in journals were also funded by the European Commission 14,16 .…”

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confidence: 99%

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When enough is enough

Hansen

Baun

2012

Nature Nanotech

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confidence: 87%

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confidence: 99%

When enough is enough

Hansen

Baun

2012

Nature Nanotech

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“…Subsequently, it was found that when silica nanospheres covered as low as 0.02% of the surface of the bilayers, the electrophysiological approach was able to detect bilayer current change caused by nanoparticle adsorption to lipid bilayers. Another electrical approach 25 quantified nanoparticle adsorption to membrane by detecting capacitive increase of suspended planar lipid bilayers.…”

Section: Experimental Techniquesmentioning

confidence: 99%

Centrifugation-based assay for examining nanoparticle–lipid membrane binding and disruption

Bothun

2014

Analyst

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Physical disruption of cellular membranes arising from interactions with engineered nanoparticles is an important, but poorly understood aspect of nanotoxicology and nanomedicine. Model cellular membranes (i.e. lipid bilayers) can be used to identify interaction mechanisms, and most studies have largely focused on lipid bilayers supported on solid planar or spherical substrates. While useful and informative, these systems do not accurately represent an intact cell membrane because they restrict the elastic motion of the bilayer and the capacity for mechanical changes.Free standing bilayers are preferred, but add complexity. Given the importance of nanoparticle-membrane interactions in nanotoxicology and nanomedicine, and the vast range in nanoparticle composition, size, shape, and surface functionalization, there is a need to develop techniques that can rapidly and inexpensively analyze the membranenanoparticle activity by using free standing or unsupported membranes.This work develops a centrifugation-based assay that can analyze the membrane-nanoparticle activity as a function of nanoparticle surface functionalization, membrane lipid composition, and monovalent salt concentration (NaCl). Free standing, unsupported vesicles were used to gain relevant information on elastic membrane deformation and vesicle destabilization due to nanoparticle binding. Silver nanoparticles were chosen due to their widespread biological applications and surface plasmon resonance (SPR) properties. UV-vis based centrifugation assay, coupled with cryo-TEM and DLS analysis, was proposed to screen nanoparticle-membrane interactions; silver nanoparticles binding ratio RSPR was calculated as a function of Ag nanoparticle coating and vesicle composition. Study showed that strong electrostatic attraction led to significant sedimentation, vesicle / membrane disruption and higher RSPR value; in contrast, systems that exhibited weak or no electrostatic attraction did not show significant sedimentation, membrane disruption or high RSPR value. The centrifugation assay provides a rapid and straightforward way to screen nanoparticlemembrane interactions.iv ACKNOWLEDGMENTS

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“…Because of these antibacterial properties, Ag NPs are suitable for a wide range of uses that include drinking water treatment, drug delivery, silver-embedded clothing, food-industry, paints and cosmetics. Moreover, their unique plasmon-resonance properties make them interesting for bio-sensing and imaging [4].…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

Broggi¹,

Ponti²,

Giudetti

et al. 2013

BioNanoMaterials

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Silver nanoparticles (Ag NPs) are one of the most common nanomaterials present in nanotechnologybased products. Here, the physical chemical properties of Ag NPs suspensions of 44 nm, 84 nm and 100 nm sizes synthesized in our laboratory were characterized. The NM-300 material (average size of 17 nm), supplied by the Joint Research Centre Nanomaterials Repository was also included in the present study. The Ag NPs potential cytotoxicity was tested on the Balb3T3 cell line by the Colony Forming Efficiency assay, while their potential morphological neoplastic transformation and genotoxicity were tested by the Cell Transformation Assay and the micronucleus test, respectively. After 24 h of exposure, NM-300 showed cytotoxicity with an IC50 of 8 µM (corresponding to 0.88 µg/mL) while for the other nanomaterials tested, values of IC50 were higher than 10 µM (1.10 µg/mL). After 72 h of exposure, Ag NPs showed size-dependent cytotoxic effect with IC50 values of 1.5 µM (1.16 µg/mL) for NM-300, 1.7 µM (1.19 µg/mL) for Ag 44 nm, 1.9 µM (0.21 µg/mL) for Ag 84 nm and 3.2 µM (0.35 µg/mL) for Ag 100 nm. None of the Ag NPs tested was able to induce either morphological neoplastic transformation or micronuclei formation.

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Silver nanoparticle applications and human health

Cited by 1,156 publications

References 74 publications

When enough is enough

When enough is enough

Centrifugation-based assay for examining nanoparticle–lipid membrane binding and disruption

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

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