Wabaidur and Alam: Moxifloxacin Analysis using Ru(bipy) 3
2+-Ce(IV) Chemiluminescence System Silver nanoparticles were synthesized and applied to sensitize Ru(bipy) 3
2+-Ce(IV) chemiluminescence system for the quantitative estimation of moxifloxacin. Prior to the experiment, silver nanoparticles were prepared using the well-reputed aqueous-gaseous phase reaction between ammonia and solution of silver nitrate. The utilization of silver nanoparticle was found to have enhanced effect on the weak chemiluminescence intensity of Ru(bipy) 3
2+-Ce(IV) system. Additionally, the enrichment of the intensity was linearly proportional to the concentration of target compound. The effects of different experimental parameters were checked to obtain best results. Under the ideal experimental conditions, the linearity of the proposed method was obtained over the moxifloxacin concentration range of 5.6×10 -9 -2.2×10 -6 M with the correlation coefficient (r 2 ) value 0.9998. The detection limit was found to be 1.5×10-9 M and the relative standard deviation calculated from five replicate measurements was 2.1%. The interference from various foreign species for the analysis of moxifloxacin was tested since they may suppress or enhance the chemiluminescence signal. The obtained quality parameters and no significant interference effect on the intensity confirm that the method is reproducible, simple and could be applied for the successful evaluation of moxifloxacin in commercial tablet and human urine.