Silver nanoparticle-induced expression of proteins related to oxidative stress and neurodegeneration in an <i>in vitro</i> human blood-brain barrier model

Khan, Asif Iqbal; Korzeniowska, Barbara; Gorshkov, Vladimir; Tahir, Muhammad; Schrøder, Henrik Daa; Skytte, Lilian; Rasmussen, Kaare Lund; Khandige, Surabhi; Møller‐Jensen, Jakob; Kjeldsen, Frank

doi:10.1080/17435390.2018.1540728

Cited by 60 publications

(30 citation statements)

References 98 publications

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“…With respect to astrocytes, a lack of a significant proteome response to NP exposures might be caused by a lesser concentration of nanomaterials as discussed. This observation is also supported by studies reporting no influence, at the protein expression level, on astrocytes exposed to silver NPs grown in 3D cell cultures 39.…”

supporting

confidence: 69%

“…37 Since metallothioneins in the human central nervous system are primarily expressed in astrocytes and there are very few reports showing that these proteins are present in the endothelium, the same mechanism may be responsible for the observed differences in nanotoxicity in our study. [38][39][40] Regarding platinum NPs, we detected much less sensitivity for either cell line. This agrees with the literature; platinum NPs feature excellent biocompatibility in various in vitro cell culture models.…”

Section: Resultsmentioning

confidence: 73%

“…60 Surprisingly, single-type NP exposure conditions provoke immunosuppressive-mediated response in endothelial cells. Among metal NPs reported to induce this response, only gold NPs have considerable size dependence, favoring particles  10 nm.Unlike gold NPs, silver NPs are generally recognized to raise the inflammation level in various human cells, including the endothelial cells of the human BBB 39,[61][62][63]. The reason(s) for this difference in immune response between single-type NP and NP co-exposure conditions are not clear from our study, but further underline the importance of considering synergetic effects when assessing NP impacts on cells.…”

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confidence: 73%

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The Cytotoxicity of Metal Nanoparticles Depends on Their Synergistic Interactions

Korzeniowska

Fonseca

Gorshkov

et al. 2020

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confidence: 69%

Section: Resultsmentioning

confidence: 73%

mentioning

confidence: 73%

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The Cytotoxicity of Metal Nanoparticles Depends on Their Synergistic Interactions

Korzeniowska

Fonseca

Gorshkov

et al. 2020

Part & Part Syst Charact

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“…These materials are normally classified based on morphology, size or chemical characteristics.NPs can be synthesized through top-down or bottom-up processes. The first method consists of mechanical, physical or chemical processes to reduce bulk materials into NPs; the second, more commonly used method one and the most used, requires molecules, ions or atoms to obtain the nanoparticles [6][7][8]. Depending on their physicochemical properties, NPs are classified as carbon-based, metallic, ceramics, semiconductor, polymeric and lipid-based [8].…”

mentioning

confidence: 99%

Genotoxicity of Silver Nanoparticles

et al. 2020

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Silver nanoparticles (AgNPs) are widely used in diverse sectors such as medicine, food, cosmetics, household items, textiles and electronics. Given the extent of human exposure to AgNPs, information about the toxicological effects of such products is required to ensure their safety. For this reason, we performed a bibliographic review of the genotoxicity studies carried out with AgNPs over the last six years. A total of 43 articles that used well-established standard assays (i.e., in vitro mouse lymphoma assays, in vitro micronucleus tests, in vitro comet assays, in vivo micronucleus tests, in vivo chromosome aberration tests and in vivo comet assays), were selected. The results showed that AgNPs produce genotoxic effects at all DNA damage levels evaluated, in both in vitro and in vivo assays. However, a higher proportion of positive results was obtained in the in vitro studies. Some authors observed that coating and size had an effect on both in vitro and in vivo results. None of the studies included a complete battery of assays, as recommended by ICH and EFSA guidelines, and few of the authors followed OECD guidelines when performing assays. A complete genotoxicological characterization of AgNPs is required for decision-making.

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“…In fact, CNS treatments for infectious diseases caused by brain-eating amoebae (Acanthamoeba species, Balamuthia mandrillaris, and Naegleria fowleri) have been proposed using these NPs [28]. However, very few studies have been performed using AgNPs as drug delivery systems as a treatment of CNS neurons, especially due to several safety-related aspects [29][30][31]. But these safety issues are not only associated with AgNPs; in fact, in spite of all of the mentioned advantageous properties of inorganic NPs, the majority still have major drawbacks and have been shown to be particularly critical for the brain, as they can induce neurotoxicity, neuroinflammation and can permanently alter the BBB permeability [23,32,33].…”

Section: Inorganic Nanosystemsmentioning

confidence: 99%

Breaking Barriers: Bioinspired Strategies for Targeted Neuronal Delivery to the Central Nervous System

et al. 2020

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Central nervous system (CNS) disorders encompass a vast spectrum of pathological conditions and represent a growing concern worldwide. Despite the high social and clinical interest in trying to solve these pathologies, there are many challenges to bridge in order to achieve an effective therapy. One of the main obstacles to advancements in this field that has hampered many of the therapeutic strategies proposed to date is the presence of the CNS barriers that restrict the access to the brain. However, adequate brain biodistribution and neuronal cells specific accumulation in the targeted site also represent major hurdles to the attainment of a successful CNS treatment. Over the last few years, nanotechnology has taken a step forward towards the development of therapeutics in neurologic diseases and different approaches have been developed to surpass these obstacles. The versatility of the designed nanocarriers in terms of physical and chemical properties, and the possibility to functionalize them with specific moieties, have resulted in improved neurotargeted delivery profiles. With the concomitant progress in biology research, many of these strategies have been inspired by nature and have taken advantage of physiological processes to achieve brain delivery. Here, the different nanosystems and targeting moieties used to achieve a neuronal delivery reported in the open literature are comprehensively reviewed and critically discussed, with emphasis on the most recent bioinspired advances in the field. Finally, we express our view on the paramount challenges in targeted neuronal delivery that need to be overcome for these promising therapeutics to move from the bench to the bedside.

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Silver nanoparticle-induced expression of proteins related to oxidative stress and neurodegeneration in an in vitro human blood-brain barrier model

Abstract: Frank Kjeldsen (2019) Silver nanoparticle-induced expression of proteins related to oxidative stress and neurodegeneration in an invitro human blood-brain barrier model,

Cited by 60 publications

References 98 publications

The Cytotoxicity of Metal Nanoparticles Depends on Their Synergistic Interactions

The Cytotoxicity of Metal Nanoparticles Depends on Their Synergistic Interactions

Genotoxicity of Silver Nanoparticles

Breaking Barriers: Bioinspired Strategies for Targeted Neuronal Delivery to the Central Nervous System

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