Silver nanoparticle toxicity in silkworms: Omics technologies for a mechanistic understanding

Chen, Liang; Xu, Meng; Gu, Jie; Fan, Weiqiang; Abdlli, Nouara; Peprah, Frank Addai; Wang, Niannian; Zhu, Feifei; Lu, Ping; Ma, Shangshang; Chen, Keping

doi:10.1016/j.ecoenv.2019.01.055

Cited by 35 publications

(14 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The midgut is the crucial part of the insect digest system and also regarded as a vital immune organ for exogenous factors and pathogens (Chen et al, 2019;Wang et al, 2013). Previous research has shown that the F I G U R E 6 Survival curve of G. pyloalis treated with chlorfenapyr (a) or phoxim (b) after GpPGRPs were silenced midgut of Anticarsia gemmatalis would be damaged by chlorantraniliprole (Castro et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…In terms of immunisation, the expression of the defensin C gene was increased by Trypanosoma cruzi infection (Araújo et al, 2021; Vieira et al, 2016). As an intermediate metabolic organ, the fat body plays an essential role in immunity, metabolic detoxification, and nutrient storage, which is similar to the mammalian liver (Arrese & Soulages, 2010; Chen et al, 2019; Mao et al, 2019; Verdú et al, 2020). In terms of metabolic detoxification, the fat body is related to multifarious detoxification enzymes such as GSTs, P450s, and CXEs (Wang et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A role of peptidoglycan recognition protein in mediating insecticide detoxification in Glyphodes pyloalis

Jiang

Ding

Liu

et al. 2021

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Glyphodes pyloalis Walker has become one of the most significant mulberry pests, and it has caused serious economic losses in major mulberry growing regions in China. Peptidoglycan recognition proteins (PGRPs) are responsible for initiating and regulating immune signalling pathways in insects. However, their roles responding to chemical pesticides is still less known. This study aimed to investigate the possible detoxication function of GpPGRP‐S2 and GpPGRP‐S3 in G. pyloalis in response to chlorfenapyr and phoxim. The chlorfenapyr and phoxim treatment significantly induced the expression level of GpPGRP‐S3 at 48 h. In addition, the expression levels of GpPGRP‐S2 and GpPGRP‐S3 in the chlorfenapyr/phoxim treatment group were significantly higher in midgut than those in the control group at 48 h. The results of the survival experiment showed that silencing either GpPGRP‐S2 or GpPGRP‐S3 would not influence the survival rate of G. pyloalis which treated with phoxim, however, silencing GpPGRP‐S2 or GpPGRP‐S3 would cause G. pyloalis to be more easily killed by chlorfenapyr. The expression of carboxylesterase GpCXE1 was significantly induced by chlorfenapyr/phoxim treatment, while it was suppressed once silenced GpPGRP‐S2 followed with chlorfenapyr treatment or silenced GpPGRP‐S3 followed with phoxim treatment. These results might suggest that under the chlorfenapyr/phoxim treatment condition, the connection between GpPGRPs and detoxification genes in insect was induced to maintain physiological homeostasis; and these results may further enrich the mechanisms of insects challenged by insecticides.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A role of peptidoglycan recognition protein in mediating insecticide detoxification in Glyphodes pyloalis

Jiang

Ding

Liu

et al. 2021

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

show abstract

“…The digestive system of silkworm is a large tube that runs through the center of the body cavity from the mouth to the anus, which includes the foregut (mouth, throat, esophagus), midgut (the most developed part of the digestive system, accounting for 78% of the total length of the digestive tract, which exchanges substances in the blood), and hindgut (small intestine, colon, rectum) [21][22]. So the midgut was selected to study the influence of two sized BN NSs on the cell morphology of silkworm.…”

Section: The Intake Of Bn Nss By Silkwormsmentioning

confidence: 99%

In vivo toxicity evaluation of boron nitride nanosheets with different sizes by silkworm model

Andoh¹,

Liu²,

Ma³

2021

Preprint

View full text Add to dashboard Cite

Boron nitride nanosheets (BN NSs), a novel material with great potential in biomedical applications, have attracted great attention due to its extraordinary properties. A crucial issue is the toxicity of BN NSs, which depends greatly on various factors, including size. The size may affect viability of cells due to the interactions between BN NSs and cell membranes. In this study, two kinds of silkworms (qiufeng × baiyu, Nistari 7019) were used as models to investigate the toxicity of BN NSs with different sizes (BN NSs-1: thickness of 41.5 nm, average diameter of about 200 nm; BN NSs-2: thickness of 48.2 nm, average diameter of about 500 nm) from the levels of animal entirety (silkworm mortality, silkworm growth, cocoons) and tissues. The results show that exposure to different sized BN NSs causes no obvious adverse effects on the growth or tissues of silkworm. This study has performed size-dependent in vivo toxicity evaluation of BN NSs and provided safety information to enrich the database for better application of BN NSs. Further studies should be carried out to discover the biosafety of diverse sizes and shapes BN NSs.

show abstract

“…Fourth, silver nanoparticles are toxic and should be used with caution (189,190). They can affect silkworms' metabolic cycle, signal transduction, apoptosis, and ion transport, weakening metabolic function and increasing energy storage and utilization (191). Silver nanoparticles can also degrade the ability of silkworms to withstand oxidative stress, interfere with programmed cell death, and attenuate the expression of detoxification proteins.…”

Section: Mechanically Enhanced Silkmentioning

confidence: 99%

Transgenic and Diet-Enhanced Silk Production for Reinforced Biomaterials: A Metamaterial Perspective

Leem

Fraser

Kim

2020

Annu. Rev. Biomed. Eng.

View full text Add to dashboard Cite

Silk fibers, which are protein-based biopolymers produced by spiders and silkworms, are fascinating biomaterials that have been extensively studied for numerous biomedical applications. Silk fibers often have remarkable physical and biological properties that typical synthetic materials do not exhibit. These attributes have prompted a wide variety of silk research, including genetic engineering, biotechnological synthesis, and bioinspired fiber spinning, to produce silk proteins on a large scale and to further enhance their properties. In this review, we describe the basic properties of spider silk and silkworm silk and the important production methods for silk proteins. We discuss recent advances in reinforced silk using silkworm transgenesis and functional additive diets with a focus on biomedical applications. We also explain that reinforced silk has an analogy with metamaterials such that user-designed atypical responses can be engineered beyond what naturally occurring materials offer. These insights into reinforced silk can guide better engineering of superior synthetic biomaterials and lead to discoveries of unexplored biological and medical applications of silk.

show abstract

Silver nanoparticle toxicity in silkworms: Omics technologies for a mechanistic understanding

Cited by 35 publications

References 49 publications

A role of peptidoglycan recognition protein in mediating insecticide detoxification in Glyphodes pyloalis

A role of peptidoglycan recognition protein in mediating insecticide detoxification in Glyphodes pyloalis

In vivo toxicity evaluation of boron nitride nanosheets with different sizes by silkworm model

Transgenic and Diet-Enhanced Silk Production for Reinforced Biomaterials: A Metamaterial Perspective

Contact Info

Product

Resources

About