Silver nanoparticle treatment ameliorates biliary atresia syndrome in rhesus rotavirus inoculated mice

Zhang, Ruizhong; Lin, Zefeng; Lui, Vincent Chi Hang; Wong, Kenneth K.; Tam, Paul Kwong Hang; Lee, Puiyan; Lok, Chun Nam; Lamb, Jonathan R.; Chen, Yan; Xia, Huimin

doi:10.1016/j.nano.2016.11.013

Cited by 23 publications

(17 citation statements)

References 39 publications

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“…Although the histological examination by H&E staining revealed reduced immunogenicity of all the decellularized slices, the AgNPs group showed a significant decrease (p < .05) in the early inflammatory response compared with those of the other groups, which might reflect the anti-inflammatory effect of the AgNPs. According to previous studies, AgNPs could reduce inflammation by decreasing proinflammatory cytokines expressions, scavenging free radicals and altering inflammatory signals in an in vitro model, and could decrease allergic, inflammatory and/or post-surgical adhesion in in vivo models [38][39][40][41].…”

Section: Discussionmentioning

confidence: 98%

Silver nanoparticles improve structural stability and biocompatibility of decellularized porcine liver

Saleh

Ahmed

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this study, we evaluated structural improvements and biocompatibility of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver slices were decellularized and then loaded with AgNPs (100 nm) after optimization of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide. The results indicated that AgNPs improved the ultra-structure of DLs' collagen fibres with good porosity and increased DLs' resistance against in vitro degradation with good cytocompatibility. AgNPs decreased the host inflammatory reaction against implanted porcine DL slices in vivo and increased the polarization of M2 macrophages. Thus, structural and functional improvements of Porcine DLs could be achieved using AgNPs.

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Section: Discussionmentioning

confidence: 98%

Silver nanoparticles improve structural stability and biocompatibility of decellularized porcine liver

Saleh

Ahmed

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…It is a fairly safe bet that there will be an increasingly important and significant proportion from Asia . From a standing start in the early 2000s, much of the current BA research already originates from Chinese institutions . Perhaps, surprisingly given the relatively huge numbers of infants being treated by centers in Beijing and Shanghai , the accent has been on basic science and laboratory‐based studies.…”

Section: Discussionmentioning

confidence: 99%

Biliary atresia: a scientometric analysis of the global research architecture and scientific developments

Friedmacher

Ford

Davenport

2019

J Hepato Biliary Pancreat

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Biliary atresia (BA) is a rare cholangiopathy of largely unknown etiology and unpredictable outcome. There has been an increasing number of BA-related publications, which may challenge researchers to determine their actual scientific value. This study aimed to evaluate the global research activity and developments relating to BA using a combination of scientometric methodologies and visualization tools. A comprehensive search strategy for the Web of Science TM database was designed to obtain bibliographic data on scientific BA publications for the timespan 1900-2018. Research output of countries, institutions, individual authors and collaborative networks was analyzed. Semi-qualitative research measures including citation rate and h-index were assessed. Choropleth mapping and network diagrams were used to visualize results. In total, 4,459 publications on BA were identified (88.5% in English), originating from 63 countries. The largest number was published by the USA (n = 991; 22.2%), Japan (n = 667; 15.0%) and the UK (n = 294; 6.6%). The USA combined the highest number of cooperation articles (n = 140). The most productive collaborative network was established between the USA and Canada (n = 17). Scientific papers from the UK received the highest average citation rate (16.7), whereas the USA had the highest country-specific h-index (59). Eighty-eight (2.0%) items were published under the auspices of multicenter consortiums and registries. The most productive institutions and authors were based in the USA, the UK, Japan, France, Canada and Taiwan. BArelated research has constantly been progressing, becoming more multidisciplinary but with main research endeavors concentrated in a few high-income countries. Studies into pathogenesis of BA remain uncommon, but are sorely needed to foster true scientific progress with this rare disease. Hence, international collaborative and translational research should be strengthened to allow further evolution in this field.

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“…A RRV strain (MMU 18006) was purchased from the American Type Culture Collection (Manassas, VA, USA), and amplified in MA104 cells. The experimental model of BA was established as described in previous study (10). Briefly, neonatal mice were injected with 20 µl of 1.2x10 5 pfu/ml of RRV or supernatant of MA104 cell culture medium (controls) intraperitoneally within 24 h of birth.…”

Section: Methodsmentioning

confidence: 99%

Antifibrotic potential of bone marrow‑derived mesenchymal stem cells in biliary atresia mice

et al. 2018

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Biliary atresia (BA) is a rare and severe disease that affects infants where a fibroinflammatory process destroys the bile ducts, leading to fibrosis and biliary cirrhosis, and mortality if untreated. Bone marrow‑derived mesenchymal stem cells (BMMSCs) have been considered as a promising therapy in fibrotic diseases. The aim of the present was to investigate the anti‑fibrotic roles of BMMSC transplantation in a BA mouse model. Mouse BA models were established by Rhesus rotavirus administration to neonatal mice. The results revealed that the liver enzyme and bilirubin metabolism levels, and the levels of the oxidative stress marker malondialdehyde (MDA) and the fibrosis marker were all increased in the BA model, while the liver tissue levels of superoxide dismutase and glutathione peroxidase were reduced. The hematoxylin and eosin and Masson's trichrome staining revealed severe liver fibrosis and collagen accumulation in BA livers. However, these indicators were all reversed once the BA mice were administered the BMMSC inoculation. In conclusion, the present study demonstrated the anti‑fibrotic potential of BMMSCs in BA mice, which may provide a novel approach to ameliorate the fibrotic response in BA patients.

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Silver nanoparticle treatment ameliorates biliary atresia syndrome in rhesus rotavirus inoculated mice

Cited by 23 publications

References 39 publications

Silver nanoparticles improve structural stability and biocompatibility of decellularized porcine liver

Silver nanoparticles improve structural stability and biocompatibility of decellularized porcine liver

Biliary atresia: a scientometric analysis of the global research architecture and scientific developments

Antifibrotic potential of bone marrow‑derived mesenchymal stem cells in biliary atresia mice

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