Silver nanoparticles: a positive factor for in vitro flowering and fruiting of purple passion fruit (Passiflora edulis Sim f. edulis)

Phong, Truong Hoai; Hieu, Tran Van; Tùng, Hoàng Thanh; Mai, Nguyen Thi Nhu; Khai, Hoang Dac; Cường, Đỗ Mạnh; Luận, Vũ Quốc; Nam, Nguyễn Bá; Nhựt, Dương Tấn

doi:10.1007/s11240-022-02361-x

Cited by 18 publications

(6 citation statements)

References 55 publications

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“…By modulating a wide array of signalling and metabolic processes in the shoot, NMs may have important effects on different processes of plant vegetative and reproductive development. Studies with various types of NMs (including carbon NMs, metallic/metal oxide NPs, and polymeric NPs) have reported positive effects of these nanostructures on the production of flowers, fruits, and seeds of many plant species, resulting in increased yield ( Jaberzadeh et al , 2013 ; Khodakovskaya et al , 2013 ; Tarafdar et al , 2014 ; Pereira et al , 2019 ; Phong et al , 2022 ). For example, Gupta et al (2022) demonstrated that the foliar application of ZnO and F 3 O 4 NPs to cucumber ( Cucumis sativus L.) plants increased the seed yield and quality.…”

Section: Nms Affect Plants At the Organ And Organism Levelsmentioning

confidence: 99%

“…Additionally, NMs have been used in plant in vitro cultures as novel elicitors to enhance the synthesis of secondary metabolites, with applications in pharmaceutical, cosmetic, and food industries ( Rivero-Montejo et al , 2021 ). There are other applications of NMs in plant in vitro cultures, including the induction of callus production, somatic embryogenesis, organogenesis, biomass accumulation, and flower/fruit production ( Khan et al , 2019 ; Mahajan et al , 2022 ; Phong et al , 2022 ).…”

Section: Application Of Theoretical Knowledge About Plant–nm Interact...mentioning

confidence: 99%

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Multilevel approach to plant–nanomaterial relationships: from cells to living ecosystems

Oliveira

Seabra

Kondak

et al. 2023

Journal of Experimental Botany

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Due to their unique properties, nanomaterials (NMs) behave peculiarly in biosystems. Regarding plants, the interactions of NMs can be interpreted on a spatial scale: from local interactions in cells to systemic effects on whole plants and on ecosystems. Interpreted on a time scale, the effects of NMs on plants may be immediate or subsequent. At the cellular level, the composition and structure of the cell wall and membranes are modified by NMs, promoting internalization. The effects of NMs on germination and seedling physiology and on the primary and secondary metabolisms in the shoot are realized at organ and organism levels. Nanomaterials interact with the beneficial ecological partners of plants. The effects of NMs on plant growth-promoting rhizobacteria and legume-rhizobia symbiosis can be stimulating or inhibitory, depending on the concentration and type of NM. Nanomaterials exert a negative effect on arbuscular mycorrhiza, and vice versa. Pollinators are exposed to NMs, which may affect plant reproduction. The substances released by the roots influence the availability of NMs in the rhizosphere and components of plant cells trigger internalization, translocation, and transformation of NMs. Understanding of the multilevel and bidirectional relationship between plants and NMs is of great relevance in practice.

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Section: Nms Affect Plants At the Organ And Organism Levelsmentioning

confidence: 99%

Section: Application Of Theoretical Knowledge About Plant–nm Interact...mentioning

confidence: 99%

Multilevel approach to plant–nanomaterial relationships: from cells to living ecosystems

Oliveira

Seabra

Kondak

et al. 2023

Journal of Experimental Botany

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“…Passion fruit ( Passiflora edulis ), also known as passiflora, is a perennial evergreen vine that grows mainly in tropical and subtropical regions due to its nutritional, medicinal and ornamental values. It belongs to the genus Passiflora , the largest genus in the Passifloraceae family, and originated in South America ( Fischer and Rezende, 2008 ; Xia et al., 2021 ; Yu et al., 2021 ; Phong et al., 2022 ). Passion fruit has been gaining popularity as a flavor in both drinks and foods as it’s rich in vitamins, antioxidants, and plant compounds that could benefit human health.…”

Section: Introductionmentioning

confidence: 99%

Cotyledon peeling method for passion fruit protoplasts: a versatile cell system for transient gene expression in passion fruit (Passiflora edulis)

Wang

Liu

et al. 2023

Front. Plant Sci.

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Passion fruit (Passiflora edulis) is a perennial evergreen vine that grows mainly in tropical and subtropical regions due to its nutritional, medicinal and ornamental values. However, the molecular biology study of passion fruit is extremely hindered by the lack of an easy and efficient method for transformation. The protoplast transformation system plays a vital role in plant regeneration, gene function analysis and genome editing. Here, we present a new method (‘Cotyledon Peeling Method’) for simple and efficient passion fruit protoplast isolation using cotyledon as the source tissue. A high yield (2.3 × 107 protoplasts per gram of fresh tissues) and viability (76%) of protoplasts were obtained upon incubation in the enzyme solution [1% (w/v) cellulase R10, 0.25% (w/v) macerozyme R10, 0.4 M mannitol, 10 mM CaCl2, 20 mM KCl, 20 mM MES and 0.1% (w/v) BSA, pH 5.7] for 2 hours. In addition, we achieved high transfection efficiency of 83% via the polyethylene glycol (PEG)-mediated transformation with a green fluorescent protein (GFP)-tagged plasmid upon optimization. The crucial factors affecting transformation efficiency were optimized as follows: 3 μg of plasmid DNA, 5 min transfection time, PEG concentration at 40% and protoplast density of 100 × 104 cells/ml. Furthermore, the established protoplast system was successfully applied for subcellular localization analysis of multiple fluorescent organelle markers and protein-protein interaction study. Taken together, we report a simple and efficient passion fruit protoplast isolation and transformation system, and demonstrate its usage in transient gene expression for the first time in passion fruit. The protoplast system would provide essential support for various passion fruit biology studies, including genome editing, gene function analysis and whole plant regeneration.

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“…Passion fruit (Passiflora edulis), also known as passiflora, is a perennial evergreen vine that grows mainly in tropical and subtropical regions due to its nutritional, medicinal and ornamental values. It belongs to the genus Passiflora, the largest genus in the Passifloraceae family, and originated in South America (Fischer and Rezende, 2008;Xia et al, 2021;Yu et al, 2021;Phong et al, 2022). Passion fruit has been gaining popularity as a flavor in both drinks and foods as it's rich in vitamins, antioxidants, and plant compounds that could benefit human health.…”

Section: Introductionmentioning

confidence: 99%

Cotyledon Peeling Method for Passion Fruit Protoplasts: a versatile cell system for transient gene expression in passion fruit (Passiflora edulis)

Wang

Liu

et al. 2023

Preprint

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Passion fruit (Passiflora edulis) is a perennial evergreen vine that grows mainly in tropical and subtropical regions due to its nutritional, medicinal and ornamental values. However, the molecular biology study of passion fruit is extremely hindered by the lack of an easy and efficient method for transformation. The protoplast transformation system plays a vital role in plant regeneration, gene function analysis and genome editing. Here, we present a new method (Cotyledon Peeling Method) for simple and efficient passion fruit protoplast isolation using cotyledon as the source tissue. A high yield (2.3 X 107 protoplasts per gram of fresh tissues) and viability (76%) of protoplasts were obtained upon incubation in the enzyme solution [1% (w/v) cellulase R10, 0.25% (w/v) macerozyme R10, 0.4 M mannitol, 10 mM CaCl2, 20 mM KCl, 20 mM MES and 0.1% (w/v) BSA, pH 5.7] for 2 hours. In addition, we achieved high transfection efficiency of 83% via the polyethylene glycol (PEG)-mediated transformation with a green fluorescent protein (GFP)-tagged plasmid upon optimization. The crucial factors affecting transformation efficiency were optimized as follows: 3 ug of plasmid DNA, 5 min transfection time, PEG concentration at 40% and protoplast density of 100 X 104 cells/ml. Furthermore, the established protoplast system was successfully applied for subcellular localization analysis of multiple fluorescent organelle markers and protein-protein interaction study. Taken together, we report a simple and efficient passion fruit protoplast isolation and transformation system, and demonstrate its usage in transient gene expression for the first time in passion fruit. The protoplast system would provide essential support for various passion fruit biology studies, including genome editing, gene function analysis and whole plant regeneration.

show abstract

Silver nanoparticles: a positive factor for in vitro flowering and fruiting of purple passion fruit (Passiflora edulis Sim f. edulis)

Cited by 18 publications

References 55 publications

Multilevel approach to plant–nanomaterial relationships: from cells to living ecosystems

Multilevel approach to plant–nanomaterial relationships: from cells to living ecosystems

Cotyledon peeling method for passion fruit protoplasts: a versatile cell system for transient gene expression in passion fruit (Passiflora edulis)

Cotyledon Peeling Method for Passion Fruit Protoplasts: a versatile cell system for transient gene expression in passion fruit (Passiflora edulis)

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