Silver nanoparticles mediated by Callistemon citrinus extracts and their antimalaria, antitrypanosoma and antibacterial efficacy

Plants are reservoir for potentially useful bioactive compounds, and owing to the rising occurrences of drug resistance to malaria parasites, there is a need to discover and develop new phytochemicals in plant that can be used as antimalarial agents. In this study, we gave a detailed description of the phytochemicals present in both ethyl acetate and methanolic extracts of Callistemon citrinus (C. citrinus) using Gas Chromatography-Mass Spectrometry (GC-MS) analysis; both extracts were also evaluated for their in vitro antimalarial, antitrypanosomal, and cytotoxicity activities against Trypanosoma brucei brucei (T. b brucei) parasites, Plasmodium falciparum (P. falciparum) malaria parasites 3D7 strain, and human cervix adenocarcinoma cells (HeLa cells); in addition, the antimicrobial and antioxidant efficacies were determined using standard methods. Both extracts were characterized by a high amount of fatty acids (52.88 and 62.48%). The ethyl acetate extract exhibited a greater activity with minimum inhibitory concentration (MIC) values ranging from 0.025 to 0.10 mg/mL while the methanol extract ranged from 0.025 to 0.15 mg/mL. Both extracts were bactericidal to Escherichia coli ATCC 35150 (E. coli) and Pseudomonas aeruginosa ACC (P. aeruginosa). Qualitative and quantitative phytochemical screenings conducted for both extracts revealed the presence of alkaloids, glycosides, saponins, steroids, and triterpenoids, fat and oils, flavonoids, phenols, and tannins in varying amounts. Both crude extracts exhibited antitrypanosomal potentials with an IC50 of 6.6/9.7 μg/mL and antiplasmodial activities with an IC50 of 8.4/13.0 μg/mL. Conclusion from this study indicates that apart from the folkloric uses of this plant in traditional settings, the extracts possess a broad spectrum of antimicrobial, antitrypanosomal, and antimalarial activities and some pharmaceutically essential bioactive components with remarkable antioxidant capacities that may be used in the synthesis of novel drugs for the management of different varieties of ailments.

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“…The reagent resazurin is usually reduced to resorufin by living cells. Pentamidine was used as a positive control [46].…”

Section: Methodsmentioning

confidence: 99%

“…Statistical analysis data was analysed using Microsoft Excel and reported as the mean ± standard deviation of triplicate determinations. In addition to this, nonlinear regression using Prism 5 for Windows, Version 5.02 (Graph Pad Software, Inc) program, was used to resolve IC 50 from the dose-response curve [46].…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Constituents, Antioxidant, Cytotoxicity, Antimicrobial, Antitrypanosomal, and Antimalarial Potentials of the Crude Extracts of Callistemon citrinus

Larayetan

Ololade

Ogunmola³

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…The AgNPs produced IC50 of 62.04 and 71.16 μg/ml against CQ-s and CQ-r strains. Larayetan et al [194] reported the synthesis of AgNPs with crude extracts of Callistemon citrinus plant and the AgNPs displayed antiplasmodial activities with IC50 ranging from 2.99-534 μg/ml. AgNPs and AuNPs were synthesized by both leaf and bark extract of S. jambos and their antiplasmodial potential against chloroquine sensitive (3D7) and resistant (Dd2) strain of Plasmodium falciparum was investigated by using 24h schizont maturation assay.…”

Section: Mosquitocidal Larvicidal Antiplasmo-dial and Trypanocidal mentioning

confidence: 99%

“…Although, there are very scanty reports on the use of nanoparticles in the treatment of trypanosomiasis, spherical AgNPs (4-9 nm) and AuNPs (7-22 nm) have been reported for the potential activity against Trypanosoma brucei [198]. AgNPs biosynthesized using the aqueous crude extracts of aerial parts of Callistemon citrinus plant was reported to display antitrypanosoma activities with IC50 of 107.30 μg/ml [194]. Also, prodigiosin, a bioactive pigments extracted from cultures of Serratia marcescens and Chromobacterium violaceum was used for the synthesis of silver and gold nanoparticles, and this was reported to display in vitro growth inhibition of Trypanosoma brucei gambiense although, the activi-ties obtained were lower than that of the pigment itself [199].…”

Section: Mosquitocidal Larvicidal Antiplasmo-dial and Trypanocidal mentioning

confidence: 99%

Biomedical Applications of Green Synthesized-Metallic Nanoparticles: A Review

Lateef

Elegbede

Akinola

et al. 2019

PAJOLS

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Advances in the field of nanoscience and nanotechnology to modulate materials at nanoscale level has continued to have great impact on different disciplines of science and engineering as well as agriculture and medical fields. The surge in the utilization of benign and non-toxic biomolecules to engineer enhanced biocompatible nanomaterials has contributed to a large extent to the applications of nanomaterials in healthcare; an emerging sub-discipline termed nanomedicine. Among the several nanomaterials that have been produced, metallic nanoparticles occupy prime position owing to their optical, catalytic and biological characteristics. These nanoparticles, which are of different types existing singly as mono-metallic nanoparticles or in two or more metals as alloys have found extremely useful applications as antimicrobial, antioxidant, anticancer, antidiabetic, anticoagulant and thrombolytic agents amongst others. In this review, an attempt is made to project the different applications of green synthesized metallic nanoparticles in diverse areas of biomedicine, including the discussion of issues of toxicity and prospects of nanotechnology in biomedical field.

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“…have as well demonstrated phytosynthesis of AgNPs using Kigelia africana with approximate size of 10 nm that inhibited P. aeruginosa, K. pneumoniae, and C. albicans Okeniyi et al (2018). employed Tectona grandis leaf extract to produce AgNPs that acted on E. coli, Serratia sp., P. aeruginosa, Bacillus sp., and Micrococcus varians Larayetan et al (2019a). used the extract of aerial part of Callistemon citrinus to synthesize AgNPs with good antibacterial activities against E. coli 0157:H7, Salmonella typhi, Vibrio alginolyticus, S. aureus, Listeria ivanovii, Mycobacterium smegmatis and Staphylococcus enteritis.…”

mentioning

confidence: 99%

Green Nanotechnology in Nigeria: The Research Landscape, Challenges and Prospects

Elegbede¹,

Lateef

2019

Annals of Science and Technology

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In this treatise, we examined the activities of researchers of Nigeria descent in the frontier areas of nanoscience and nanotechnology, with a focus on green nanotechnology. The exploration of literature published by scholars were reviewed and compartmentalized on the basis of applications of the nanomaterials. It can be concluded that the level of activities in this area is expanding owing to the emergence of more published works since the beginning of 2010. However, in comparison with research outputs from other developing African countries such as South Africa and Egypt, activities in green nanotechnology are still at low ebb in Nigeria. Issues that are contributory to the slow pace were identified and appropriate solutions in terms of improved funding of education, enactment of national policy on nanotechnology, curriculum development, international cooperation as well as human resource development among others were discussed.

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Silver nanoparticles mediated by Callistemon citrinus extracts and their antimalaria, antitrypanosoma and antibacterial efficacy

Cited by 39 publications

References 58 publications

Phytochemical Constituents, Antioxidant, Cytotoxicity, Antimicrobial, Antitrypanosomal, and Antimalarial Potentials of the Crude Extracts of Callistemon citrinus

Phytochemical Constituents, Antioxidant, Cytotoxicity, Antimicrobial, Antitrypanosomal, and Antimalarial Potentials of the Crude Extracts of Callistemon citrinus

Biomedical Applications of Green Synthesized-Metallic Nanoparticles: A Review

Green Nanotechnology in Nigeria: The Research Landscape, Challenges and Prospects

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