Simazine perturbs the maturational competency of mouse oocyte through inducing oxidative stress and DNA damage

Shang, Jianzhou; Li, Shiru; Li, Xiaoqing; Zhou, Yuting; Ma, Xiang; Liu, Lu; Niu, Dong; Duan, Xing

doi:10.1016/j.ecoenv.2021.113105

Cited by 10 publications

(8 citation statements)

References 46 publications

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“…BPF was reported to induce oxidative stress in various cells, which was well known to damage oocytes quality 34 . Thus, we speculated that BPF exposure could induce oxidative stress in mouse oocytes, which may further cause meiotic defects.…”

Section: Resultsmentioning

confidence: 94%

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Bisphenol F exposure affects mouse oocyte in vitro maturation through inducing oxidative stress and DNA damage

Ding

Chen

Ahmad

et al. 2022

Environmental Toxicology

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Bisphenol F (BPF), a substitute for bisphenol A (BPA), is progressively used to manufacture various consumer products. Despite the established reproductive toxicity of BPF, the underlying mechanisms remain to elucidate. This in‐vitro study deep in sighted the BPF toxicity on mouse oocyte meiotic maturation and quality. After treating oocytes with BPF (300 μM), the oocyte meiotic progression was blocked, accentuated by a reduced rate in the first polar body extrusion (PBE). Next, we illustrated that BPF induced α‐tubulin hyper‐acetylation disrupted the spindle assembly and chromosome alignment. Concurrently, BPF resulted in severe oxidative stress and DNA damage, which triggered the early apoptosis in mouse oocytes. Further, altered epigenetic modifications following BPF exposure were proved by increased H3K27me3 levels. Concerning the toxic effects on spindle structure, oxidative stress, and DNA damage in mouse oocytes, BPF toxicity was less severe to oocyte maturation and spindle structure than BPA and induced low oxidative stress. However, compared with BPA, oocytes treated with BPF were more prone to DNA damage, indicating not less intense or even more severe toxic effects of BPF than BPA on some aspects of oocytes maturation. In brief, the present study established that like wise to BPA, BPF could inhibit meiotic maturation and reduce oocyte quality, suggesting it is not a safe substitute for BPA.

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Section: Resultsmentioning

confidence: 94%

“…BPF was reported to induce oxidative stress in various cells, which was well known to damage oocytes quality. 34 Thus, we speculated…”

Section: Bpf Exposure Increased the Ros Level And Dna Damage In Mouse...mentioning

confidence: 98%

Bisphenol F exposure affects mouse oocyte in vitro maturation through inducing oxidative stress and DNA damage

Ding

Chen

Ahmad

et al. 2022

Environmental Toxicology

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“…If there is an imbalance in mitochondrial fission and fusion processes, this can cause mitochondrial dysfunction, the production of ROS, and apoptosis [33]. Simazine exposure has been shown to cause mitochondrial dysfunction and oxidative stress, both of which are associated with early oocyte apoptosis [34]. PFOA-induced oocyte deterioration was caused by mitochondrial dysfunction and apoptosis in the F1 offspring [35].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Arp2/3 Complex Inhibition on Cytoskeleton Dynamics and Mitochondrial Function during Goat Oocyte Meiosis

Pan

Zhang

et al. 2023

Animals

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F-actin is of critical importance in oocyte meiotic maturation. Actin assembly and its dynamics are mainly regulated by actin nucleation factors. The actin-related protein complex 2/3 (Arp2/3) is responsible for the organization of F-actin filaments. However, the role of Arp2/3 complex in goat oocytes has not been fully elucidated. Our findings demonstrate that Arp2/3 complex activity is necessary for the maturation of goat oocytes. The Arp2/3 complex-specific inhibitor CK666 impairs the maturation of goat oocytes and alters the genes associated with cumulus expansion, both of which suggest that normal meiosis is affected. Arp2, one of the subunits of the Arp2/3 complex, was found to be mainly accumulated at the oocyte cortex and to co-localize with F-actin during goat oocyte maturation in our results. Thus, we further investigated the cytoskeleton dynamics and found that Arp2/3 complex inhibition disrupts the F-actin assembly and spindle organization. Further analysis revealed that, in addition to direct effects on the cytoskeleton, Arp2/3 complex could also induce ROS accumulation and oxidative stress by disrupting mitochondrial distribution and function, ultimately increasing the rate of early apoptosis in goat oocytes. Our study provides evidence that the Arp2/3 complex is a key regulator of goat oocyte maturation through its regulation of the cytoskeleton dynamics and mitochondrial function.

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“…Mitochondria are the major sources of ROS, and mitochondrial dysfunction often causes excessive ROS production, resulting in oxidative stress and apoptosis [ 29 , 30 , 31 ]. Previous studies have shown that mitochondrial dynamics is an essential quality control method involved in mitochondrial fission and fusion [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

SIRT4 Expression Ameliorates the Detrimental Effect of Heat Stress via AMPK/mTOR Signaling Pathway in BMECs

Ding

Wang

Xia

et al. 2022

IJMS

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Sirtuin 4 (SIRT4), a member of the SIRT family, has been reported to be a key factor involved in antioxidant defense in mitochondria. This study aimed to explore the potential molecular mechanism via which SIRT4 regulates heat stress-induced oxidative stress and lactoprotein synthesis in bovine mammary epithelial cells (BMECs). Our results showed that SIRT4 was significantly decreased in heat stressed mammary tissue. Depletion of SIRT4 in BMECs induced the generation of ROS, which, as exhibited by the decreased activity of antioxidant enzymes, changed mitochondrial morphology through mediating protein and mRNA levels related to mitochondrial fission and fusion. Moreover, we found that depletion of SIRT4 or stress conditions inhibited the expression of milk proteins, as well as lipid and glucose synthesis-related genes, and activated the AMPK/mTOR signaling pathway. Increased SIRT4 expression was found to have the opposite effect. However, blocking the AMPK/mTOR signaling pathway could inhibit the regulatory function of SIRT4 in milk synthesis-related gene expression. In summary, our results suggest that SIRT4 may play critical roles in maintaining mammary gland function by regulating the AMPK/mTOR signaling pathway in dairy cows, indicating that SIRT4 may be a potential molecular target for curing heat stress-induced BMEC injury and low milk production in dairy cows.

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Simazine perturbs the maturational competency of mouse oocyte through inducing oxidative stress and DNA damage

Cited by 10 publications

References 46 publications

Bisphenol F exposure affects mouse oocyte in vitro maturation through inducing oxidative stress and DNA damage

Bisphenol F exposure affects mouse oocyte in vitro maturation through inducing oxidative stress and DNA damage

The Impact of Arp2/3 Complex Inhibition on Cytoskeleton Dynamics and Mitochondrial Function during Goat Oocyte Meiosis

SIRT4 Expression Ameliorates the Detrimental Effect of Heat Stress via AMPK/mTOR Signaling Pathway in BMECs

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