Similar Connotation in Chronic Hepatitis B and Nonalcoholic Fatty Liver Patients with Dampness-Heat Syndrome

Dai, Jianye; Sun, Shujun; Cao, Jun; Zhao, Yu; Cao, Huijuan; Zheng, Ningning; Fang, Juemin; Wang, Yang; Zhang, Wei; Zhang, Yongyu; Hu, Yiyang; Cao, Zhiwei

doi:10.1155/2013/793820

Cited by 10 publications

(14 citation statements)

References 15 publications

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“…Protein precipitation and metabolite extraction were conducted as previously described (Dai et al, 2013 ). Each 100-μL serum sample was combined with 10 μL of heptadecanoic acid (1 mg/mL) and 300 μL of solvent (methanol: chloroform, 3:1, V/V).…”

Section: Methodsmentioning

confidence: 99%

Gut Microbial Dysbiosis Is Associated with Altered Hepatic Functions and Serum Metabolites in Chronic Hepatitis B Patients

et al. 2017

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Chronic hepatitis B (CHB) is a global epidemic disease that results from hepatitis B virus (HBV) infection and may progress to severe liver failure, including liver fibrosis, cirrhosis and hepatocellular carcinoma. Previous evidence has indicated that the dysbiosis of gut microbiota occurs after liver virus infection and is associated with severe liver disease. The aim of this study is to elucidate the compositional and functional characteristics of the gut microbiota in early-stage CHB and to understand their influence on disease progression. We investigated the gut microbial composition of stool samples from 85 CHB patients with low Child-Pugh scores and 22 healthy controls using the Illumina MiSeq sequencing platform. Furthermore, the serum metabolome of 40 subjects was measured by gas chromatography mass spectrometry. Compared with the controls, significant alteration in the gut microbiota was observed in the CHB patients; 5 operational taxonomic units (OTUs) belonging to Actinomyces, Clostridium sensu stricto, unclassified Lachnospiraceae and Megamonas were increased, and 27 belonging to Alistipes, Asaccharobacter, Bacteroides, Butyricimonas, Clostridium IV, Escherichia/Shigella, Parabacteroides, Ruminococcus, unclassified Bacteria, unclassified Clostridiales, Unclassified Coriobacteriaceae, unclassified Enterobacteriaceae, unclassified Lachnospiraceae and unclassified Ruminococcaceae were decreased. The inferred metagenomic information of gut microbiota in CHB showed 21 enriched and 17 depleted KEGG level-2 pathways. Four OTUs, OTU38 (Streptococcus), OTU124 (Veillonella), OTU224 (Streptococcus), and OTU55 (Haemophilus), had high correlations with hosts' hepatic function indices and 10 serum metabolites, including phenylalanine and tyrosine, which are aromatic amino acids that play pathogenic roles in liver disease. In particular, these 4 OTUs were significantly higher in patients with higher Child-Pugh scores, who also showed diminished phenylalanine and tryptophan metabolisms in the inferred gut metagenomic functions. These compositional and functional changes in the gut microbiota in early-stage CHB patients suggest the potential contributions of gut microbiota to the progression of CHB, and thus provide new insight into gut microbiota-targeted interventions to improve the prognosis of this disease.

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Section: Methodsmentioning

confidence: 99%

Gut Microbial Dysbiosis Is Associated with Altered Hepatic Functions and Serum Metabolites in Chronic Hepatitis B Patients

et al. 2017

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“…In TCM, spleen regulates transportation and transformation of nutrition through converting food and water into nutritions, and Damp-heat constitution was caused by disordered function of spleen and abnormal water metabolism. In Damp-heat constitution subjects, sodium elements were accumulated in intracellular fluid, leading to symptoms of edema [ 27 ], a common manifestation of diabetes and its complication, diabetic retinopathy. Therefore, Damp-heat constitution could also be used to estimate the risk of diabetes.…”

Section: Discussionmentioning

confidence: 99%

Association of TCM body constitution with insulin resistance and risk of diabetes in impaired glucose regulation patients

You

Zhang

Feng

et al. 2017

BMC Complement Altern Med

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BackgroundImpaired glucose regulation (IGR) patients have increased risk of type 2 diabetes mellitus (T2DM). Identifying relevant risk factors in IGR subjects could facilitate early detection and prevention of IGR progression to diabetes. This study investigated the association between Traditional Chinese Medicine (TCM) body constitution and serum cytokines, and whether body constitution could independently predict diabetes in IGR subjects.MethodPatients with IGR (n = 306) received a blood test and their body constitution type was assessed using a body constitution questionnaire (BCQ). Serum levels of cytokines were measured by ELISA. Patients were followed up for at least three years, and their status of diabetes were recorded. Multivariate logistic regression was used to estimate odds ratios (ORs) of diabetes for body constitution.ResultsPhlegm-damp, Damp-heat and Qi-deficiency were three most common unbanlenced constitutions among IGR subjects. Phlegm-damp and Damp-heat constitution subjects showed higher serum levels of interleukin 6 (IL-6), tumour necrosis factor-α (TNF-α), leptin and lower serum levels of adiponectin (P<0.05). Qi-deficiency constitution subjects showed higher serum levels of leptin and lower serum levels of adiponectin, glucagon-like peptide-1 (GLP-1) and gastric inhibitory polypeptide (GIP) (P<0.05). Subjects with Phlegm-damp or Damp-heat constitution demonstrated a significantly higher risk of diabetes (P<0.05).ConclusionPhlegm-damp and Damp-heat TCM body constitution are strongly associated with abnormal serum cytokines, and could potentially serve as a predictor of diabetes in IGR subjects. Body constitution can help to identify IGR subjects who are at a high risk of progression to diabetes.

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“…All serum, feces, and tissues were prepared for metabolomics analysis. For serum samples, the preparation was the same as in our previous reports (Sun et al, 2012; Dai et al, 2013b,c). For liver tissue, 50 mg of tissue was homogenized by 150 μL of normal saline.…”

Section: Methodsmentioning

confidence: 99%

“…And for feces, 80 mg of fecal sample was transferred into a screw tube with 320 μL of normal saline and extracted with ultrasonic treatment for 20 min. Then, the derivatization process was performed as in our previous publications (Sun et al, 2012; Dai et al, 2013b,c).…”

Section: Methodsmentioning

confidence: 99%

The Medicinal Plant Pair Bupleurum chinense-Scutellaria baicalensis – Metabolomics and Metallomics Analysis in a Model for Alcoholic Liver Injury

Dang

Sun

et al. 2019

Front. Pharmacol.

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Traditional Chinese Medicine (TCM), a complex natural herbal medicine system, has increasingly attracted attention from all over the world. Most research has illustrated the mechanism of TCM based on the active components or single herbs. It was fruitful and effective but far from satisfactory as it failed to gain insights into the interactivity and combined effects of TCM. In this work, we used Bupleurum chinense ( B. chinense DC, a species in the genus Bupleurum , family Apiaceae ) and Scutellaria baicalensis ( S. baicalensis Georgi, a species in the genus Scutellaria , family Lamiaceae ), an herbal pair in TCM, to illustrate the combined effect. We compared the diverse effects between the B. chinense-S. baicalensis herbal pair and its compositions in an animal model of Alcoholic Liver Injury to highlight the advantages of the formula. Biochemical and histological indicators revealed that the effect of B. chinense-S. baicalensis was better than its individual parts. Furthermore, metabolite profiling of the serum, liver tissue, and feces were conducted to reveal that the herbal pair largely presented its effects through enhanced tissue penetration to maintain liver-located intervention with less global and symbiotic disturbance. Furthermore, we analyzed the distribution of the metal elements in extracts of the serum and liver tissue and found that the herbal pair significantly regulated the distribution of endogenous selenium in liver tissue. As selenium plays an important role in the anti-oxidative and hepatoprotective effects, it may be the reason for combined effects in BS formula. This research could open new perspectives for exploring the material basis of combined effects in natural herbal medicine.

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Similar Connotation in Chronic Hepatitis B and Nonalcoholic Fatty Liver Patients with Dampness-Heat Syndrome

Cited by 10 publications

References 15 publications

Gut Microbial Dysbiosis Is Associated with Altered Hepatic Functions and Serum Metabolites in Chronic Hepatitis B Patients

Gut Microbial Dysbiosis Is Associated with Altered Hepatic Functions and Serum Metabolites in Chronic Hepatitis B Patients

Association of TCM body constitution with insulin resistance and risk of diabetes in impaired glucose regulation patients

The Medicinal Plant Pair Bupleurum chinense-Scutellaria baicalensis – Metabolomics and Metallomics Analysis in a Model for Alcoholic Liver Injury

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