Simple and sensitive lead ion detection based on difference of gold monomer ratio using dark field microscope as readout system

An algorithm-assisted automated identification and enumeration system for sensitive hydrogen sulfide sensing under dark field microscopy

“…Then, excess sulfuric acid was added to the tube. Owing to the low pH value, almost all sulfides form H 2 S molecules rather than HS − or S. 2–49 The low solubility made the H 2 S molecule volatilize and come into contact with AuNPs-N 3 -Alk and further reduced the azido group to an amino group, which has been proven by many works. 50–53 Therefore, the cuprous-catalysed click chemistry reaction was blocked, resulting in single AuNPs in the solution.…”

“…[29][30][31][32] These methods were developed based on a general image processing software (e.g., ImageJ) that collects the scattering signal changes and quickly corresponds to the target concentration. Our group also performed research by using Image J software as a data processor [33][34][35] for poisonous substance and biomolecule detection. However, the mechanical identification of nanoparticle scattering spots by their inherent size and central colour would result in mistaken identification.…”