Simple and sensitive liquid chromatography–tandem mass spectrometry method for determination of the S(+)- and R(−)-enantiomers of baclofen in human plasma and cerebrospinal fluid

Goda, Ryoya; Murayama, Nobuyuki; Fujimaki, Yuko; Sudo, Koji

doi:10.1016/j.jchromb.2003.11.025

Cited by 35 publications

(26 citation statements)

References 23 publications

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“…It is used in multiple sclerosis or spasticity caused by spinal or cerebral injury [31]. The results of screening of chiral selectors towards FITC-labeled baclofen are shown in Figs.…”

Section: Screening Of Chiral Selectors By Four-channel Microchip Elecmentioning

confidence: 99%

A multichannel electrophoresis microchip platform for rapid chiral selector screening

et al. 2008

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This study presents a four-channel electrophoresis chip platform, featuring double-cross hydrostatic sample injection, for rapid chiral selector screening. This platform needs only five electrodes to drive microchip electrophoresis in four separate channels for screening four chiral selectors at a time. To demonstrate the performance of this screening platform, eight neutral CDs and their derivatives as chiral selectors were screened towards two FITC-labeled chiral compounds. The screening could be accomplished in less than 2 min. Dimethyl-beta-CD and hydroxypropyl-alpha-CD was demonstrated to be the appropriate selectors for FITC-norfenefrine and FITC-baclofen, respectively. The established platform is easy to operate and suitable for rapid screening process, which is expected to be a potential platform for high-throughput screening of chiral selectors.

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“…It is used in multiple sclerosis or spasticity caused by spinal or cerebral injury [31]. The results of screening of chiral selectors towards FITC-labeled baclofen are shown in Figs.…”

Section: Screening Of Chiral Selectors By Four-channel Microchip Elecmentioning

confidence: 99%

A multichannel electrophoresis microchip platform for rapid chiral selector screening

et al. 2008

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“…Goda et al [18] use an ultrafiltration membrane and ultra-centrifugation to clean-up plasma and cerebrospinal fluid (CSP) samples. Their results showed that no matrix effects and no co-eluting peaks interfere with the enantiomeric quantification of baclofen, a muscle relaxant.…”

Section: Plasma and Serum Samplementioning

confidence: 99%

Liquid separation techniques coupled with mass spectrometry for chiral analysis of pharmaceuticals compounds and their metabolites in biological fluids

Erny

Cifuentes

2006

Journal of Pharmaceutical and Biomedical Analysis

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Determination of the chiral composition of drugs is nowadays a key step in order to determine purity, activity, bioavailability, biodegradation, etc, of pharmaceuticals. In this manuscript, works published for the last 5 years on the analysis of chiral drugs by liquid separation techniques coupled with mass spectrometry are reviewed. Namely, chiral analysis of pharmaceuticals including e.g., antiinflammatories, antihypertensives, relaxants, etc, by liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry are included. The importance and interest of the analysis of the enantiomers of the active compound and its metabolites in different biological fluids (plasma, urine, cerebrospinal fluid, etc) are also discussed.

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“…For determination of Bac enantiomers in human plasma and cerebrospinalfluid, Goda et al employed a chiral CROWNPAK CR(+)® column [15]. Chiral stationary phases (CSPs), based on macrocyclic antibiotic teicoplanin (Chirobiotic T) [16] and those based on ligand exchange (Phenomenex chirex®) [17], have been used for enantioseparation of Bac enantiomers in human plasma.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of three strong UV-absorbing Naproxen-based chiral derivatizing agents and their application for enantioseparation of Baclofen by RP-HPLC

Batra¹,

Bhushan²

2015

Acta Chromatographica

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Summary.A sensitive and stereoselective high-performance liquid chromatography (HPLC) method based on diastereomer formation was developed and fully validated for the simultaneous determination of the two enantiomers of Baclofen. (S)-Naproxen was used to prepare three chiral derivatizing reagents (CDRs) which were used for synthesizing diastereomers of Baclofen. The diastereomers so synthesized were separated on C 18 column under reversed phase conditions using a binary mixture of acetonitrile and triethylammonium phosphate, with UV detection at 220 nm. The results obtained for enantioseparation of Baclofen using the three CDRs have been compiled and compared. The conditions for derivatization and chromatographic separation have been optimized. The method was validated for linearity, repeatability, limits of detection, and limit of quantification.

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Simple and sensitive liquid chromatography–tandem mass spectrometry method for determination of the S(+)- and R(−)-enantiomers of baclofen in human plasma and cerebrospinal fluid

Cited by 35 publications

References 23 publications

A multichannel electrophoresis microchip platform for rapid chiral selector screening

A multichannel electrophoresis microchip platform for rapid chiral selector screening

Liquid separation techniques coupled with mass spectrometry for chiral analysis of pharmaceuticals compounds and their metabolites in biological fluids

Synthesis of three strong UV-absorbing Naproxen-based chiral derivatizing agents and their application for enantioseparation of Baclofen by RP-HPLC

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