Simple and tunable surface coatings via polydopamine for modulating pharmacokinetics, cell uptake and biodistribution of polymeric nanoparticles

Liu, Jingshuo; Xu, Huaizhe; Tang, Xing; Xu, Jinghua; Zeng, Jin; Li, Hui; Wang, Shihan; Gou, Jingxin; Jin, Xiangshu

doi:10.1039/c7ra01354j

Cited by 35 publications

(27 citation statements)

References 48 publications

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“…The functionalization is based on a Schiff base type or Michael addition reaction between amine groups of BSA and catechol/quinine of polydopamine, which can be achieved through overnight incubation at room temperature. [ 45–47 ] After several washing steps with HyClone water, the particle's hydrodynamic diameter slightly increased to 513 ± 6 nm which gave a first indication that coating with BSA was successful. In addition, the zeta‐potential turned slightly negative after the coating procedure (‐19.4 ± 0.8 mV in HyClone water), providing further proof that the BSA coating was successful (Figure S2, violet bar, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells

Harizaj

Wels

Raes

et al. 2021

Adv Funct Materials

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Safe and efficient production of chimeric antigen receptor (CAR)‐T cells is of crucial importance for cell‐based cancer immunotherapy. Physical transfection methods have quickly gained in importance in the context of transfecting T‐cells, since they are readily compatible with different cell types and a broad variety of cargo molecules. In particular, nanoparticle‐sensitized photoporation has been introduced in recent years as a gentle yet efficient method to transiently permeabilize cells, allowing subsequent entry of external cargo molecules into the cells. Gold nanoparticles (AuNPs) have been used the most as photothermal sensitizers because they can easily form laser‐induced vapor nanobubbles, a photothermal phenomenon that is shown to be particularly efficient for permeabilizing cells. However, as AuNPs are not biodegradable, clinical translation is hampered. Here, for the first time, the possibility to form laser‐induced vapor nanobubbles from biocompatible polymeric nanoparticles is reported. Compared to electroporation, the most used physical transfection method for T cells, 2.5 times more living mRNA transfected human T cells are obtained via photoporation sensitized by polydopamine nanoparticles. This shows that photoporation is a viable approach for efficiently producing therapeutic engineered T‐cells at a throughput easily exceeding 105 cells per second.

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Section: Resultsmentioning

confidence: 99%

Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells

Harizaj

Wels

Raes

et al. 2021

Adv Funct Materials

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“…ABTS is used widely as a diffusional redox mediator for BOD or laccase bioelectrocatalytic activation. Moreover, polydopamine layers can conjugate lysine groups that are available on the protein surface; thus, BOD can be attached covalently to the electrode‐modified polymeric layer. An examination of the free lysine groups on the BOD (MvBOD, PDB, 2XLL) surface showed that Lys 21, 44, 181, 214, and 314 are in close vicinity to the T1 site and may be conjugated to the polymeric layer, which would improve the enzyme orientation with respect to the electrode.…”

Section: Resultsmentioning

confidence: 99%

Bismuth Vanadate/Bilirubin Oxidase Photo(bio)electrochemical Cells for Unbiased, Light‐Triggered Electrical Power Generation

2020

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The construction of bias‐ and donor‐free photobioelectrochemical cells for the generation of light‐triggered electrical power is presented. The developed oxygen reduction biocathodes are based on bilirubin oxidase (BOD) that originates from Myrothecium verrucaria (MvBOD) and a thermophilic Bacillus pumilus (BpBOD). Methods to entrap the BOD with 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) redox molecules in a polydopamine layer are presented. A pH‐independent, positively charged pyrenebetaine linker was synthesized, utilized, and led to a threefold improvement to the bioelectrocatalytic current. Both the developed polydopamine/ABTS/MvBOD and the pyrenebetaine/BpBOD biocathodes were further coupled with BiVO4/cobalt phosphate water‐oxidation photoanodes to construct biotic/abiotic photobioelectrochemical cells, which generated power outputs of 0.74 and 0.85 mW cm−2, respectively. The presented methods are versatile, show the strength of biotic/abiotic hybrids, and can be further used to couple different redox enzymes with electrodes.

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“…Thus, as an excellent functional constituent material, PDA has an immense application scope, especially in the biomedicine. [ 10–14 ]…”

Section: Introductionmentioning

confidence: 99%

Polydopamine‐Incorporated Nanoformulations for Biomedical Applications

Zheng

Ding

Gao

2020

Macromolecular Bioscience

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Polydopamine (PDA), a pigment in natural melanin, has attracted considerable attention because of its excellent optical properties, extraordinary adhesion, and good biocompatibility, which make it a promising material for application in energy, environmental, and biomedical fields. In this review, PDA‐incorporated nanoformulations are focused for biomedical applications such as drug delivery, bioimaging, and tumor therapy. First, the recent advances in PDA‐incorporated nanoformulations for drug delivery are discussed. Further, their application in boimaging, such as fluorescence imaging, photothermal imaging, and photoacoustic imaging, is reviewed. Next, their therapeutic applications, including chemotherapy, photodynamic therapy, photothermal therapy, and synergistic therapy are discussed. Finally, other biomedical applications of PDA‐incorporated nanoformulations such as biosensing and clinical diagnosis are briefly presented. Finally, the biomedical applications of PDA‐incorporated nanoformulations along with their prospects are summarized.

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Simple and tunable surface coatings via polydopamine for modulating pharmacokinetics, cell uptake and biodistribution of polymeric nanoparticles

Abstract: A strategy that can modulate biological response such as pharmacokinetics, cell uptake and biodistribution of NPs simply by tunable coatings was established.

Cited by 35 publications

References 48 publications

Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells

Photoporation with Biodegradable Polydopamine Nanosensitizers Enables Safe and Efficient Delivery of mRNA in Human T Cells

Bismuth Vanadate/Bilirubin Oxidase Photo(bio)electrochemical Cells for Unbiased, Light‐Triggered Electrical Power Generation

Polydopamine‐Incorporated Nanoformulations for Biomedical Applications

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