Simple and ultrastable all-inclusive pullulan tablets for challenging bioassays

Jahanshahi‐Anbuhi, Sana; Kannan, Balamurali; Leung, Vincent; Pennings, Kevin; Liu, Meng; Carrasquilla, Carmen; White, Dawn; Li, Yingfu; Pelton, Robert; Brennan, John D.; Filipe, Carlos D. M.

doi:10.1039/c5sc04184h

Cited by 39 publications

(30 citation statements)

References 40 publications

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“…This explanation would be consistent with previously published results, which note trehalose’s common usage as a desiccation protectant that is often used during lyophilisation 21,29,45 . Moreover, we have previously shown that enzyme mobility is restricted in a pullulan film, even at 60 °C 41 . The restriction of mobility afforded by the pullulan glass matrix results in enhanced thermal stability.…”

Section: Discussionmentioning

confidence: 94%

Thermal Stabilization of Viral Vaccines in Low-Cost Sugar Films

Leung

Mapletoft

Zhang

et al. 2019

Sci Rep

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Most currently available vaccines, particularly live vaccines, require the cold chain, as vaccine efficacy can be significantly hampered if they are not stored in a temperature range of 2–8 °C at all times. This necessity places a tremendous financial and logistical burden on vaccination programs, particularly in the developing world. The development of thermally stable vaccines can greatly alleviate this problem and, in turn, increase vaccine accessibility worldwide. In this paper, we detail a simple and cost-effective method for stabilizing live vaccines that uses FDA-approved materials. To this end, we dried enveloped DNA (Herpes Simplex Virus type 2) and RNA (Influenza A virus) viral vaccines in a pullulan and trehalose mixture. The results of these studies showed that the live-attenuated HSV-2 vaccine retained its efficacy for at least 2 months of storage at 40 °C, while the inactivated influenza vaccine was able to retain its immunogenicity for at least 3 months of storage at 40 °C. This work presents a simple approach that allows thermo-sensitive vaccines to be converted into thermo-stable vaccines that do not require refrigeration, thus contributing to the improvement of vaccine deployment throughout the world.

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Section: Discussionmentioning

confidence: 94%

Thermal Stabilization of Viral Vaccines in Low-Cost Sugar Films

Leung

Mapletoft

Zhang

et al. 2019

Sci Rep

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“…The use of urease and nucleic acids in the biosensor system makes this a relevant challenge. However, recently demonstrated methods that can stabilize proteins (Jahanshahi-Anbuhi et al, 2014 , 2016 ) and nucleic acids (including RNA-cleaving DNAzymes) (Hsieh et al, 2017 ) through the use of a natural polysaccharide known as pullulan have been reported. The same approach can be used to solve the issue of reagent stability of our system.…”

Section: Resultsmentioning

confidence: 99%

Colorimetric Detection of Uranyl Using a Litmus Test

et al. 2018

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Ingestion of water containing toxic contaminants above levels deemed safe for human consumption can occur unknowingly since numerous common contaminants in drinking water are colorless and odorless. Uranyl is particularly problematic as it has been found at dangerous levels in sources of drinking water. Detection of this heavy metal-ion species in drinking water currently requires sending a sample to a laboratory where trained personnel use equipment to perform the analysis and turn-around times can be long. A pH-responsive colorimetric biosensor was developed to enable detection of uranyl in water which coupled the uranyl-specific 39E DNAzyme as a recognition element, and an enzyme capable of producing a pH change as the reporter element. The rapid colorimetric assay presented herein can detect uranyl in lake and well water at concentrations relevant for environmental monitoring, as demonstrated by the detection of uranyl at levels below the limits set for drinking water by major regulatory agencies including the World Health Organization (30 μg/L). This simple and inexpensive DNAzyme-based assay enabled equipment-free visual detection of 15 μg/L uranyl, using both solution-based and paper-based pH-dependent visualization strategies.

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“…Therefore, sensitive and specific detection of ATP is extremely urgent 3 . To date, various detection methods for ATP have been designed 4–6 . Noteworthily, electrochemical biological sensing, as one of the main detection technologies, is favored due to its simple equipment, good selectivity, fast response, low cost and broad applications 7–12 .…”

Section: Introductionmentioning

confidence: 99%

Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework

Shi

Zhang

et al. 2017

Sci Rep

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A sensitive, selective and recyclable electrochemical sensor is designed for ATP detection based on amino-functionalized metal-organic framework. The functional MOF as the sensor is constructed by one-step synthesis Ce-MOF and sequentially modified on the Au electrode and conjugated with the aptamer of ATP. The presence of target ATP leads to the conformational change of aptamer strands and strong electrochemical impedance. The electrochemical sensor can detect ATP down to 5.6 nM with the linear range of 10 nm to 1000 μM. The present study is the first report on the use of MOF as an electrochemical sensor for ATP at nM level. This strategy has been successfully applied in detection of ATP in serum of cancer patients, which reveals its potential application in clinical diagnosis.

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Simple and ultrastable all-inclusive pullulan tablets for challenging bioassays

Abstract: A glowing example: simple to use, highly stable and cost-effective luciferase/luciferin assays using rapidly solubilizing pullulan tablets.

Cited by 39 publications

References 40 publications

Thermal Stabilization of Viral Vaccines in Low-Cost Sugar Films

Thermal Stabilization of Viral Vaccines in Low-Cost Sugar Films

Colorimetric Detection of Uranyl Using a Litmus Test

Label-free Electrochemical Detection of ATP Based on Amino-functionalized Metal-organic Framework

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