Simple Eco-Friendly RP-LC Method for the Synchronous Separation of Six Widely Used Drugs in the Treatment of Cerebrovascular and Vestibular Disorders: Application for Analysis in Their Single and Combined Drug Products

Abstract

Background RP-LC is considered the most extensively used chromatographic technique in drug separation and quantification; yet its routine use shows increasing environmental challenges about energy consumption, solvent used and waste engendered. From this perception, efforts for Greening the developed RP-LC methods are focused on using of green solvents; reducing the amount of solvent used and waste produced. Objective The target of this work is to develop eco-friendly RP-LC method for the synchronous separation of six widely used drugs in the treatment of cerebrovascular and vestibular disorders namely; neurotropic Piracetam, antihistamines, Dimenhydrinate, Cinnarizine and Fluranizine, vasodilators Vincamine and Vinpocetine. Method Separation and quantification are accomplished at ambient temperature by isocratic elution mode using acetonitrile–0.2% triethylamine (85:15; by volume) as a mobile phase at a flow rate of 1.5 mL/min on Hypersil C18 column. UV detection and quantification are carried out at 225 nm. Results The method is fully validated per ICH guidelines, where all analytical parameters are within acceptable range, r > 0.9999, accuracy ≥99.55% and precision (0.02–0.97%). Conclusion The method is applied for the routine analysis of the cited drugs in their single and combined drug products and it is considered excellent in terms of greenness with respect to Eco-Scale assessment. Highlights As the routine use of RP-LC technique shows increasing environmental challenges, efforts for Greening are attempted focusing on the use of green solvents; reduce the amount of solvent used and waste produced. A simple eco-friendly RP-LC method for the synchronous separation of six widely used drugs in the treatment of cerebrovascular and vestibular disorders is developed. The method is fully validated per ICH guidelines, where all analytical parameter are within acceptable range. -The method is applied for the routine analysis of the cited drugs in their single and combined drug products.