Simple, fast and reliable CE method for simultaneous determination of ciprofloxacin and ofloxacin in human urine

Abstract

A simple, fast, and accurate capillary zone electrophoresis method has been developed for the determination of ciprofloxacin and ofloxacin. This method uses liquid–liquid extraction. Therefore, it is characterized by a very simple procedure of sample preparation but at the same time satisfactory precision and accuracy. The extraction process of the same urine sample was repeated three times. The extraction protocol was performed each time for 15 min with 1 mL of dichloromethane and chloroform mixture in a 3:1 volume ratio. A 0.1 mol/L phosphate-borate buffer (pH 8.40) was selected as the background electrolyte. UV detection was performed at 288 nm. The separation was carried out at a voltage of 16 kV, at a temperature of 25 °C. Experimentally evaluated LOQ values for ciprofloxacin and ofloxacin were 0.2 nmol/mL urine and 0.05 nmol/mL urine, respectively. For both analytes the calibration curves exhibited linearity over the entire tested concentration range of 1–6 nmol/mL urine. The precision of the method did not exceed 15%, and the recovery was in the range of 85–115%. The developed and validated procedure was applied to analyze human urine for the content of ciprofloxacin and ofloxacin.

Eco-friendly, sensitive and inventive first derivative synchronous spectrofluorimetric determination of ciprofloxacin and phenylephrine in their pure form, single and combined eye drops

“…Several analytical techniques have been evolved for analyzing CFX as; some of analytical methods were summarized in the reported review [ 6 ], spectrophotometry [ 7 – 12 ], spectrofluorimetry [ 13 , 14 ], electrochemistry [ 15 – 17 ], high-performance liquid chromatography [ 18 – 20 ], capillary electrophoresis [ 21 ] and titration [ 22 ].…”

Simultaneous Quantification of Some Fluoroquinolone Residues in Real Wastewater Effluents Using CZE

“…It occurs as a result Numerous methods for determining FQs in aquatic habitats have been developed, including HPLC [14][15][16], LC-MS/MS [17,18], and electrochemistry [19]. On the other hand, capillary electrophoresis (CE) has been applied to quantify FQs in different sample forms, including, bovine milk [20], human urine [21], animal tissues [22], pharmaceutical dosage forms [23], and water environments [24][25][26][27][28].…”

Stability-Indicating Quantification of Ciprofloxacin in the Presence of Its Main Photo-Degradation Product by CZE and UPLC: A Comparative Study

“…The major goal of this research was to optimize and validate the CZE and UPLC methods for quantifying CPF in the presence of its main photo-degradant through a comparative approach between the suggested methodologies. CPF has been determined by spectroscopy [6][7][8], liquid chromatography (LC) [9][10][11][12][13], capillary electrophoresis (CE) [14,15], and electrochemistry [16][17][18] in a variety of sample formats. For the selective quantification of the investigated medication in the presence of its various degradation products, various approaches have been used.…”