Simple High-pressure Liquid Chromatographic Determination of Trisulfapyrimidines in Human Serum

Goehl, Thomas J.; Mathur, Lalit K.; Strum, Jeffrey D.; Jaffe, James M.; Pitlick, William H.; Shah, Vinod P.; Poust, Rolland I.; Colaizzi, John L.

doi:10.1002/jps.2600670336

Cited by 19 publications

(3 citation statements)

References 16 publications

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“…T h e concentration of SMZ in the serum samples was measured by high-performance liquid chromatography according to the method of Goehl et al (1978).…”

Section: Sulfamethazine Assaymentioning

confidence: 99%

Pharmacokinetics, safety and tissue residues of sustained‐release sulfamethazine in sheep

Bulgin

Lane

Archer

et al. 1991

Vet Pharm & Therapeutics

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Concentration-time profiles and the rates of absorption, extent of distribution and half-lives of sulfamethazine (SMZ), administered intravenously, orally as a water solution and as a sustained-release formulation (CalfSpan) were determined in 10 healthy sheep. The geometric mean half-life of elimination of i.v. SMZ was 10.8 h, compared to 14.3 h for the sustained-release preparation (CalfSpan) and 4.3 h for the oral water solution. Blood levels of SMZ were at or above 50 micrograms/ml for more than 48 h for CalfSpan, for 24 h after i.v. SMZ (100 mg/kg body wt), and for less than 24 h after p.o. SMZ (100 mg/kg body wt). The mean bioavailability of the oral SMZ solution was 58.3% (AUCp.o./AUCi.v.). The estimated bioavailability of the CalfSpan preparation was 52.5%. The safety of the sustained-release preparation was tested by dosing sheep with multiples (one, three and five times) of the recommended dose (one tablet, 8 g SMZ, per 20 kg body wt), once a day for 3 days. Clinical blood chemistries showed a significant increase in serum iron, and a decrease in serum phosphorus in animals treated at the 3x and 5x dose levels. Necropsies of the 5x dose animals did not show any gross signs that could be attributed to SMZ, and histological examination of tissues from the 5x animals revealed no organ pathology. Residues of SMZ in liver, fat, kidney and skeletal muscle were measured in 20 animals that received one bolus per 20 kg body wt. The results indicate that SMZ residues are cleared rapidly, and are at or below the tolerance level of 0.1 mg/kg within 8 days after dosing so that the 18-day withdrawal time used in cattle would provide an appropriate margin of safety if used in sheep.

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“…T h e concentration of SMZ in the serum samples was measured by high-performance liquid chromatography according to the method of Goehl et al (1978).…”

Section: Sulfamethazine Assaymentioning

confidence: 99%

Pharmacokinetics, safety and tissue residues of sustained‐release sulfamethazine in sheep

Bulgin

Lane

Archer

et al. 1991

Vet Pharm & Therapeutics

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“…Fourteen blood samples were then collected over a 48-hr period, and serum was analyzed for I, 11, and 111 by a specific HPLC procedure (10).…”

Section: Methodsmentioning

confidence: 99%

In Vivo–In Vitro Correlations for Trisulfapyrimidine Suspensions

Mathur¹,

Colaizzi

Jaffe³

et al. 1983

Journal of Pharmaceutical Sciences

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“…1976;Helboe and Thomsen, 1977;Goehl et al 1978). However, only a few methods are available for the combination of sulphadiazine and tetroxoprim (Vergin and Fritschi, 1979;Vergin and Bishop, 1980;Bishop-Frcudling and Vergin, 1981; Springolo and Coppi, 1989).…”

Section: Introductionmentioning

confidence: 96%

High performance liquid chromatographic analysis of tetroxoprim and sulphadiazine in serum and urine

Alkaysi

Salem

Badwan

1991

Biomedical Chromatography

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Quantitative determination of tetroxoprim and sulphadiazine in serum and urine was performed using reversed phase high performance liquid chromatography. Protein precipitation using 10% perchloric acid was utilized for purification of serum samples while urine samples were diluted prior to analysis. The mobile phase consisted of triethylammonium acetate buffer (85%), acetonitrile (12%) and methanol (3%), with a final pH of 4.2. The eluent was monitored at 280 nm. Benzoic acid was used as an internal standard. Standardization, validation and application of the method is described.

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Simple High-pressure Liquid Chromatographic Determination of Trisulfapyrimidines in Human Serum

Cited by 19 publications

References 16 publications

Pharmacokinetics, safety and tissue residues of sustained‐release sulfamethazine in sheep

Pharmacokinetics, safety and tissue residues of sustained‐release sulfamethazine in sheep

In Vivo–In Vitro Correlations for Trisulfapyrimidine Suspensions

High performance liquid chromatographic analysis of tetroxoprim and sulphadiazine in serum and urine

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