Simple isotope dilution assay for propionic acid and isovaleric acid

Arthur, Kathleen; Hommes, F. A.

doi:10.1016/0378-4347(95)00255-h

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Of these three, we were able to confirm valerate and isovalerate, but not pentanal. In addition to these previously reported ligands, we now show that Olfr691 is broadly tuned towards carboxylic acid activation, including short and medium chain fatty acids (physiological concentrations are within the range of the ligand concentrations assayed for isovalerate (0.89±0.93 uM [50] )). Interestingly, gut bacterial metabolism is the primary physiological source of short chain fatty acids in the bloodstream, with the concentrations reported for propionate varying from 0.1–10 mM [6] .…”

Section: Discussionsupporting

confidence: 67%

Identification and Characterization of Novel Renal Sensory Receptors

et al. 2014

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Recent studies have highlighted the important roles that “sensory” receptors (olfactory receptors, taste receptors, and orphan “GPR” receptors) play in a variety of tissues, including the kidney. Although several studies have identified important roles that individual sensory receptors play in the kidney, there has not been a systematic analysis of the renal repertoire of sensory receptors. In this study, we identify novel renal sensory receptors belonging to the GPR (n = 76), olfactory receptor (n = 6), and taste receptor (n = 11) gene families. A variety of reverse transcriptase (RT)- PCR screening strategies were used to identify novel renal sensory receptors, which were subsequently confirmed using gene-specific primers. The tissue-specific distribution of these receptors was determined, and the novel renal ORs were cloned from whole mouse kidney. Renal ORs that trafficked properly in vitro were screened for potential ligands using a dual-luciferase ligand screen, and novel ligands were identified for Olfr691. These studies demonstrate that multiple sensory receptors are expressed in the kidney beyond those previously identified. These results greatly expand the known repertoire of renal sensory receptors. Importantly, the mRNA of many of the receptors identified in this study are expressed highly in the kidney (comparable to well-known and extensively studied renal GPCRs), and in future studies it will be important to elucidate the roles that these novel renal receptors play in renal physiology.

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Section: Discussionsupporting

confidence: 67%

Identification and Characterization of Novel Renal Sensory Receptors

et al. 2014

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“…In the urine of a patient with inborn errors of metabolism (IEMs), the substrate of an enzyme reaction and/or the metabolites formed secondarily via by-paths due to the accumulation of the substrate markedly increase [1][2][3][4][5][6]. Methylmalonic acidemia (MMA) is an autosomal recessive IEM in which the activity of L-methylmalonyl-CoA mutase is deficient or greatly reduced (OMIM 251000).…”

Section: Introductionmentioning

confidence: 99%

Determination of methylmalonic acid and glutaric acid in urine by aqueous‐phase derivatization followed by headspace solid‐phase microextraction and gas chromatography‐mass spectrometry

Deng²,

Zhang

2007

J of Separation Science

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In this work, a novel technique of aqueous-phase derivatization followed by headspace solid-phase microextraction and gas chromatography-mass spectrometry was developed for the determination of organic acids in urine. The analytical procedure involves derivatization of organic acids to their ethyl esters with diethyl sulfate, headspace sampling, and GC/MS analysis. The proposed method was applied to the determination of methylmalonic acid and glutaric acid in urine. The experimental parameters and method validation were studied. Optimal conditions were obtained: PDMS fiber, extraction temperature 55 degrees C, extraction time 30 min, and 60 microL of diethyl sulfate as derivatization reagent with 2 mg of the ion pairing agent tetrabutylammonium hydrogensulfate. The method was linear over three orders of magnitude, and detection limits were 21 nM for methylmalonic acid and 34 nM for glutaric acid, respectively. Consequently, in-situ derivatization/HS-SPME/GC/MS is an alternative and powerful method for determination of organic acids as biomarkers in biological fluids.

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A sensitive liquid chromatographic method for the analysis of isovaleric and valeric acids in urine as fluorescent derivatives

Chung

Chao

2007

Journal of Chromatography A

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Simple isotope dilution assay for propionic acid and isovaleric acid

Cited by 3 publications

References 13 publications

Identification and Characterization of Novel Renal Sensory Receptors

Identification and Characterization of Novel Renal Sensory Receptors

Determination of methylmalonic acid and glutaric acid in urine by aqueous‐phase derivatization followed by headspace solid‐phase microextraction and gas chromatography‐mass spectrometry

A sensitive liquid chromatographic method for the analysis of isovaleric and valeric acids in urine as fluorescent derivatives

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