Simple Method for Spectrophotometric Determination of Benzidine in Aqueous Solutions by Coupling with Β-Naphthol

Abstract

Anew simple and rapid colorimetric method has been developed for the determination of benzidine in aqueous solutions. The method is based on the formation of a pink colored azo dye by diazotization of benzidine, followed by a diazo-coupling reaction between the resulting product and β-naphthol. The maximum absorbance of azo dye at 525 nm. Beer's law was found to be obeyed in the concentration range of 0-12 µg. ml −1 with molar absorptivity 12360 L. mol −1. cm −1. The optimum reaction conditions and other analytical parameters were evaluated.

Liquid Chromatographic and validation study in separation and determination of benzidines and phenols in the main discharge point of wastewater

“…The content of benzidinic compounds in aqueous samples can be determined by various analytical instrumental methods, such as a gas chromatography(GC)/mass spectrometric assay [8], rapid colourimetry [9], fluorescence spectrographic method [10] [10],novel resonance light scattering (RLS) [11], ion selective electrodes [12] , direct injection and Ultrahigh-pressure liquid chromatography (UPLC) with fluorescence detection [13], and high-performance liquid chromatography with various detectors such as UV detector [14] , mass spectroscopy detector [15] electrochemical detector [16].…”