Simple strategy for the in vitro conservation of Alnus glutinosa (L.) Gaertn. germplasm

Abstract

The aim of this study was to develop a simple method for the medium-term storage of Alnus glutinosa (L.) Gaertn. explants obtained from trees aged 20-30 years. Several parameters were evaluated, including type of explant (shoot apex or nodal segments), pre-storage treatment (0 or 10 days after the last subculture) and duration of cold storage (3-24 months) at 2-4ºC. Explants were maintained at this temperature under dim lighting on Woody Plant Medium supplemented with 0.1 mg l-1 6-benzyladenine and 0.5 mg l-1 indole-3-acetic acid. Under these conditions, a high percentage (75-87%) of cultures remained viable after 18 months in cold cabinets. The stored material was successfully recovered and multiplied normally in the same medium, showing good growth and developing into normal shoots that were morphologically similar to those of non-stored controls. At the histological level, the main change observed was the accumulation of starch granules in cells of the shoot apex, as well as in cells located close to the vascular bundles, after 3 months of cold storage. As the duration of cold storage increased, the number and size of the starch granules decreased but cell plasmolysis and the content of lipid droplets increased. Cold damage was generalized after 24 months at 4ºC. This study provides new insights into the changes occurring in A. glutinosa during cold storage.

Conservación de plantas regeneradas in vitro y análisis de la variación somaclonal de Cinchona officinalis, Linneo

“…Por tanto la aplicación de estas herramientas biotecnológicas con fines de conservación se justifican para proteger la diversidad genética de las especies (Mohanty et al 2013;San José et al 2014). En este trabajo se evaluará la efectividad de la modificación del medio de cultivo y la adición de agentes osmótico en la conservación in vitro para limitar el crecimiento y del nitrógeno líquido (NL) (-196°C) en la conservación a largo plazo de explantos de C.…”

Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

“…On the other hand, tissue cryopreservation involves the suspension of growth for indefinite periods (Paunescu 2009;Ai et al 2012;Reed et al 2011;Sen-Rong and Ming-Hua 2012;Cruz et al 2013;Panis et al 2020;Popova et al 2023). The application of these techniques translates into a significant contribution to the conservation of species facing challenges in natural propagation (Ruta et al 2020), safeguarding the genetic diversity of the species in question (Mohanty et al 2013;San José et al 2014;Armijos-González et al 2021).…”

Genome Status of Lippia alba Polyploid Complex Long-term in vitro Cultivated

“…The in vitro technique is a powerful tool to maintain plant genetic resources. It has been widely used for a long time as an efficient alternative to preserve native and cultivated plant germplasm [1,2] and also to quickly increase the number of plants of commercial interest [3]. In addition, the possibility to modulate the production of chemical components constitutes an interesting aspect to explore in vitro cultivation [4,5,6].…”