Simple Ways to Measure Behavioral Responses of Drosophila to Stimuli and Use of These Methods to Characterize a Novel Mutant

Vang, Lar L; Medvedev, Alexei V.; Adler, Julius

doi:10.1371/journal.pone.0037495

Cited by 35 publications

(32 citation statements)

References 55 publications

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“…In the case of the repellent quinine, flies were started in a 0.1M quinine half and then they had the opportunity to go into a non-quinine half (see Vang, Medvedev, and Adler, 2012, for details of the method). The parent went into the non-quinine half but Mutant 1 and Mutant 2 did not.…”

Section: Response To Other External Stimulimentioning

confidence: 99%

“…To test response to gravity, these flies were placed into a vertical tube and pounded down, then at every minute the flies in each third of the tube were counted (see Vang, Medvedev, and Adler, 2012, for details of the method). The parent responded by climbing up while Mutants 1 and 2 climbed up 10% as well after subtraction of movement without any added stimuli.…”

Section: Response To Other External Stimulimentioning

confidence: 99%

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DrosophilaMutants that Are Motile but Respond Poorly to All Stimuli Tested: Mutants in RNA Splicing and RNA Helicase, Mutants in The Boss

Vang

Adler

2016

Preprint

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Adult Drosophila melanogaster fruit flies were placed into one end of a tube near to repellents (benzaldehyde and heat) and away from the other end containing attractants (light and a favored temperature). They escaped from the repellents and went to the attractants. Five motile mutants that failed to do that were isolated. They did not respond to any external attractants tested or external repellents tested. In addition, they did not respond well to internal sensory stimuli like hunger, thirst, and sleep. The mutants, although motile, failed to respond to stimuli at both 34°C and at room temperature. Some of the mutants have been mapped. The mutants are missing RNA splicing and RNA helicase. In addition, mutants missing information from The Boss are discussed.

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Section: Response To Other External Stimulimentioning

confidence: 99%

Section: Response To Other External Stimulimentioning

confidence: 99%

DrosophilaMutants that Are Motile but Respond Poorly to All Stimuli Tested: Mutants in RNA Splicing and RNA Helicase, Mutants in The Boss

Vang

Adler

2016

Preprint

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“…In the case of the repellent quinine, flies were started in a 0.1M quinine half and then they had the opportunity to go into a non-quinine half (see Vang et al, 2012, for details of the method). The parent went into the non-quinine half but Mutant 1 and Mutant 2 did not.…”

Section: Response To Other External Stimulimentioning

confidence: 99%

“…To test response to gravity, these flies were placed into a vertical tube and pounded down, then at every minute the flies in each third of the tube were counted (see Vang et al, 2012, for details of the method). The parent responded by climbing up while Mutants 1 and 2 climbed up 10% as well after subtraction of movement without any added stimuli.…”

Section: Response To Other External Stimulimentioning

confidence: 99%

DrosophilaMutants that Are Motile but Respond Poorly to All Stimuli Tested

Vang

Adler

2016

Preprint

Self Cite

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Adult Drosophila melanogaster fruit flies were placed into one end of a tube near to repellents (benzaldehyde and heat) and away from the other end containing attractants (light and a favored temperature). They escaped from the repellents and went to the attractants. Five motile mutants that failed to do that were isolated. They did not respond to any external attractants tested or external repellents tested. In addition, they did not respond well to internal sensory stimuli like hunger, thirst, and sleep. The mutants, although motile, failed to respond to stimuli at both 34°C and at room temperature. Some of the mutants have been mapped. It is proposed that the information from the different sensory receptors comes together at an intermediate, called "inbetween" (Inbet), that brings about a behavioral response. The Boss is defined here.

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“…Adult flies are known to react to a number of stimulants, e.g. gravity, odours, light, temperature and sound (Vang et al, 2012), which have all been applied when creating assays to evaluate the flies' behaviour, and thereby gain information on the effect of a certain drug or how the expression of transgenes impacts behaviour. In transgenic flies, by directing the protein expression to different cells or tissues, different phenotypical assays can be used to study any possible toxic effects.…”

Section: Phenotypic Analysis Of Drosophilamentioning

confidence: 99%

Amyloid-β and lysozyme proteotoxicity in Drosophila : Beneficial effects of lysozyme and serum amyloid P component in models of Alzheimer’s disease and lysozyme amyloidosis

Bergkvist¹

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Previously published papers have been re-printed with kind permission from the publishers.Cover: Drosophila melanogaster, amino acid sequence of Aβ 1-42 and sketch of a secondary protein structure inspired by human lysozyme. Liza BergkvistAmyloid-β and lysozyme proteotoxicity in Drosophila Beneficial effects of lysozyme and serum amyloid P component in models of Alzheimer's disease and lysozyme amyloidosis Alzheimers sjukdom och lysozymamyloidos samt molekyler som skulle kunna ha en positiv effekt på dessa sjukdomar studerats med hjälp av Drosophila melanogaster, bananflugan. Trots att bananflugan är ett ryggradslöst djur så finns det stora likheter mellan människors och bananflugors fundamentala biologiska mekanismer. I början av 1980-talet kom de första transgena flugorna, det vill säga flugor där man placerat in humana gener i flugans egen arvsmassa. Sedan dess har bananflugan varit en av de mest använda modellorganismerna för att studera effekten av överuttryckta humana proteiner i biologiska system; man kan se flugan som ett levande provrör. För majoriteten av proteinerna i vår kropp krävs det att de veckar sig till en tredimensionell struktur för att de ska fungera ordentligt. Men ibland blir det fel, och proteinet felveckas. Det kan till exempel vara på grund av mutationer som gör proteinet mindre stabilt. Ett felveckat protein kan då klumpa ihop sig, aggregera, med andra felveckade proteiner. I Alzheimers sjukdom, som är den vanligaste formen av demens, finns proteinaggregat i hjärnan hos patienterna. Dessa aggregat består av amyloid-β (Aβ) -peptiden, en liten peptid på runt 42 aminosyror som klipps ut från det större membranbundna proteinet AβPP av två olika enzymer, BACE1 och gamma-sekretas. I det första arbetet inkluderat i den här avhandlingen har två olika flugmodeller för Alzheimers sjukdom använts; Aβ-modellen som direkt uttrycker Aβ-peptiden samt AβPP-BACE1-modellen, där alla komponenter som behövs för att flugan ska producera Aβ-peptiden. De två olika flugmodellerna jämfördes och vi kunde se att det behövs en betydligt lägre mängd av Aβ i AβPP-BACE1-modellen för att få samma, eller till och med en större, toxisk effekt jämfört med Aβ-modellen. I det andra arbetet har dessa två flugmodeller för Alzheimers sjukdom använts igen, men nu för att studera huruvida lysozym, ett protein involverat i vårt medfödda immunsystem, kunde motverka den toxiska effekt som Aβ genererade i flugmodellerna. Vi fann att lysozym kan rädda flugorna från Aβ inducerad toxicitet, samt att Aβ och lysozym interagerar med varandra. Den andra proteinfelveckningssjukdomen som studerats i denna avhandling är lysozymamyloidos. Det är en ovanlig, ärftlig sjukdom där mutanta varianter av lysozym ger upphov till flera kilogram tunga aggregat som lägger sig runt njurar och lever, vilket tillslut leder till organsvikt. I avhandlingens tredje arbete har en flugmodell för lysozymamyloidos använts för att studera vad serum amyloid P komponenten, SAP, ett protein som finns i alla proteinaggregat man hittar inom denna sjukdomsklass, har f...

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Simple Ways to Measure Behavioral Responses of Drosophila to Stimuli and Use of These Methods to Characterize a Novel Mutant

Cited by 35 publications

References 55 publications

DrosophilaMutants that Are Motile but Respond Poorly to All Stimuli Tested: Mutants in RNA Splicing and RNA Helicase, Mutants in The Boss

DrosophilaMutants that Are Motile but Respond Poorly to All Stimuli Tested: Mutants in RNA Splicing and RNA Helicase, Mutants in The Boss

DrosophilaMutants that Are Motile but Respond Poorly to All Stimuli Tested

Amyloid-β and lysozyme proteotoxicity in Drosophila : Beneficial effects of lysozyme and serum amyloid P component in models of Alzheimer’s disease and lysozyme amyloidosis

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