Simplified immunoassay for rapid Dengue serotype diagnosis, revealing insensitivity to non-specific binding interference

Loureiro, F.C.C.L.; Neff, H.; Melcher, Elmar U. K.; Roque, Rosemary Aparecida; Figueiredo, Regina Maria Pinto de; Thirstrup, Carsten; Borre, Martin B.; Lima, Antonio Marcus Nogueira

doi:10.1016/j.sbsr.2016.10.002

Cited by 15 publications

(9 citation statements)

References 19 publications

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“… Biosensor type Analyte Materials Real sample/spiked sample Detection range LOD Year Refs. Immunosensor NS1 Gold film electrode Serum 1–100 ng/mL 0.33 ng/mL 2012 42 LSPR fiber optic sensor NS1 Gold Nanoparticles – – 1.54 nM 2013 10 Immunosensor NS1 Microspheres Serum – 5.2 ng/mL 2013 43 Impedimetric biosensor NS1 Gold electrode Neat serum 0.01–1.00 μg/mL 30 ng/mL 2015 44 SPR biosensors DENV2 Ag Gold film Serum – 4 × 10 4 particles/mL 2017 45 FITC optical biosensors NS1 Indium tin oxide Plasma 15–500 ng/mL 15 ng/mL 2018 46 Immunosensor NS1 …”

Section: Resultsmentioning

confidence: 99%

Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots

Tran

Park

2021

Sci Rep

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A highly sensitive immunosensor using streptavidin-conjugated quantum dots (QDs/SA) was developed to detect dengue biomarker of non-structural protein 1 (NS1) at very low concentration, so that it can probe dengue infection even in the early stage. The QDs/SA were first bound to biotinylated NS1 antibody (Ab) and the QDs/SA-Ab conjugates were then used to detect the NS1 antigen (Ag) in the Ag concentration range of 1 pM to 120 nM. The formation of QDs/SA-Ab and QDs/SA-Ab-Ag conjugates was confirmed by the measurements of field emission scanning electron microscopy (FF-SEM), field emission transmission electron microscopy (FE-TEM), dynamic light scattering (DLS), and zeta-potential. Fluorescence emission spectra of QDs/SA-Ab-Ag conjugates showed that the magnitude of fluorescence quenching was linearly proportional to the NS1 Ag concentration and it nicely followed the Stern–Volmer (SV) equation in phosphate buffer solution. However, in human plasma serum solution, the fluorescence quenching behavior was negatively deviated from the SV equation presumably due to interference by the serum component biomolecules, and it was well explained by the Lehrer equation. These results suggest that the current approach is promising because it is highly sensitive, fast, simple, and convenient, and thus it has a potential of application for point-of-care.

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Section: Resultsmentioning

confidence: 99%

Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots

Tran

Park

2021

Sci Rep

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“…The detection limit in samples spiked with nasal mucosa from flu-like syndrome patients was observed to be 402 copies/mL, which is significantly lower than the detection limit of conventional influenza detection approaches including rapid influenza diagnostic tests and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Similarly, Loureiro et al developed a PSPR-based immunoassay for the rapid diagnosis of dengue viral infection [49]. In this method, a neutravidin-biotin mediated monoclonal antibody is immobilized on a thin gold film as the sensing element.…”

Section: Analytical Methods For Viral Agentsmentioning

confidence: 99%

Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Choi

Lee

Son

et al. 2020

Sensors

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For the early diagnosis of several diseases, various biomarkers have been discovered and utilized through the measurement of concentrations in body fluids such as blood, urine, and saliva. The most representative analytical method for biomarker detection is an immunosensor, which exploits the specific antigen-antibody immunoreaction. Among diverse analytical methods, surface plasmon resonance (SPR)-based immunosensors are emerging as a potential detection platform due to high sensitivity, selectivity, and intuitive features. Particularly, SPR-based immunosensors could detect biomarkers without labeling of a specific detection probe, as typical immunosensors such as enzyme-linked immunosorbent assay (ELISA) use enzymes like horseradish peroxidase (HRP). In this review, SPR-based immunosensors utilizing noble metals such as Au and Ag as SPR-inducing factors for the measurement of different types of protein biomarkers, including viruses, microbes, and extracellular vesicles (EV), are briefly introduced.

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“…The diagnosis of dengue viral infection was possible by a rapid propagating surface plasmon resonance (PSPR)-based immunoassay, where a neutravidin-biotin monoclonal antibody (the sensing element) was immobilized on a thin gold film. An LoD of 2 × 10 4 particles/mL was obtained [ 64 ]. Avian influenza A H7N9 was detected by Chang et al [ 65 ].…”

Section: Surface Plasmon Resonance (Spr) Based Biosensorsmentioning

confidence: 99%

Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

Camarca

Varriale

Capo

et al. 2021

Sensors

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The purpose of this work is to provide an exhaustive overview of the emerging biosensor technologies for the detection of analytes of interest for food, environment, security, and health. Over the years, biosensors have acquired increasing importance in a wide range of applications due to synergistic studies of various scientific disciplines, determining their great commercial potential and revealing how nanotechnology and biotechnology can be strictly connected. In the present scenario, biosensors have increased their detection limit and sensitivity unthinkable until a few years ago. The most widely used biosensors are optical-based devices such as surface plasmon resonance (SPR)-based biosensors and fluorescence-based biosensors. Here, we will review them by highlighting how the progress in their design and development could impact our daily life.

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Simplified immunoassay for rapid Dengue serotype diagnosis, revealing insensitivity to non-specific binding interference

Cited by 15 publications

References 19 publications

Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots

Highly sensitive detection of dengue biomarker using streptavidin-conjugated quantum dots

Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

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