Simplified Microchip Electrophoresis for Rapid Separation of Urine Proteins

Song, Hongwei; Wang, Huimin; Ju, Saoqing; Jin, Qinghui; Jia, Chunping; Cong, Hui

doi:10.1002/jcla.21651

Cited by 7 publications

(6 citation statements)

References 30 publications

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“…Consequently, to improve and miniaturize the electrophoretic techniques, microchip electrophoresis (mCE) has also been developed as an attractive tool for intact protein analysis [167–171]. This technique can significantly reduce analysis times, in addition to reducing sample volume requirements, with an increased efficiency of resolution relative to traditional CE.…”

Section: Electrophoretic Methodsmentioning

confidence: 99%

Sample preparation and fractionation techniques for intact proteins for mass spectrometric analysis

Thomas

Thacker

Schug

et al. 2020

J of Separation Science

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The analysis of proteins in biological samples is highly desirable, given their connection to myriad biological functions and disease states, as well as the growing interest in the development of protein‐based pharmaceuticals. The introduction and maturation of “soft” ionization methods, such as electrospray ionization and matrix‐assisted laser desorption/ionization, have made mass spectrometry an indispensable tool for the analysis of proteins. Despite the availability of powerful instrumentation, sample preparation and fractionation remain among the most challenging aspects of protein analysis. This review summarizes these challenges and provides an overview of the state‐of‐the‐art in sample preparation and fractionation of proteins for mass spectrometric analysis, with an emphasis on those used for top‐down proteomic approaches. Biological fluids, particularly important for clinical and pharmaceutical applications and their characteristics are also discussed. While immunoaffinity‐based methods are addressed, more attention is given to non‐immunoaffinity‐based methods, such as precipitation, coacervation, size exclusion, dialysis, solid‐phase extraction, and electrophoresis. These techniques are presented in the context of a significant number of studies where they have been developed and utilized.

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Section: Electrophoretic Methodsmentioning

confidence: 99%

Sample preparation and fractionation techniques for intact proteins for mass spectrometric analysis

Thomas

Thacker

Schug

et al. 2020

J of Separation Science

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“…The approaches to study the function of liver [139], kidney [140][141][142][143], lacrimal gland [144,145], and thyroid gland [146] included miniaturised immunoassays, gel electrophoresis, and zone electrophoresis.…”

Section: Organ Diseases and Dysfunctionsmentioning

confidence: 99%

“…Kidney function was evaluated based on measuring the urea concentration in human serum [140], creatinine and protein concentration in urine [142,143] and artificial human serum [141].…”

Section: Kidney Functionmentioning

confidence: 99%

A decade of microchip electrophoresis for clinical diagnostics – A review of 2008–2017

Wuethrich

Quirino

2019

Analytica Chimica Acta

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A core element in clinical diagnostics is the data interpretation obtained through the analysis of patient samples. To obtain relevant and reliable information, a methodological approach of sample preparation, separation, and detection is required. Traditionally, these steps are performed independently and stepwise. Microchip capillary electrophoresis (MCE) can provide rapid and high-resolution separation with the capability to integrate a streamlined and complete diagnostic workflow suitable for the point-of-care setting. Whilst standard clinical diagnostics methods normally require hours to days to retrieve specific patient data, MCE can reduce the time to minutes, hastening the delivery of treatment options for the patients. This review covers the advances in MCE for disease detection from 2008 to 2017. Miniaturised diagnostic approaches that required an electrophoretic separation step prior to the detection of the biological samples are reviewed. In the two main sections, the discussion is focused on the technical setup used to suit MCE for disease detection and on the strategies that have been applied to study various diseases. Throughout these discussions MCE is compared to other techniques to create context of the potential and challenges of MCE. A comprehensive table categorised based on the studied disease using MCE is provided. We also comment on future challenges that remain to be addressed.

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“…Song et al. used a ME to determine protein profiles in urine samples from patients with different types of proteinuria. The procedure compared excellently with standard gel electrophoresis and was able to identify all the major protein bands in 4 min.…”

Section: Applications In Different Clinical Fieldsmentioning

confidence: 99%

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

Phillips

2017

Electrophoresis

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Recent advances in CE and microchip-CE in clinical applications: 2014 to mid-2017CE and microchip CE (ME) are powerful tools for the analysis of a number of different analytes and have been applied to a variety of clinical fields and human samples. This review will present an overview of the most recent applications of these techniques to different areas of clinical medicine during the period of 2014 to mid-2017. CE and ME have been applied to clinical chemistry, drug detection and monitoring, hematology, infectious diseases, oncology, endocrinology, neonatology, nephrology, and genetic screening. Samples examined range from serum, plasma, and urine to lest utilized materials such as tears, cerebral spinal fluid, sweat, saliva, condensed breath, single cells, and biopsy tissue. Examples of clinical applications will be given along with the various detection systems employed.

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Simplified Microchip Electrophoresis for Rapid Separation of Urine Proteins

Abstract: This newly established ME could have broad applications to determine the type of proteinuria in clinics.

Cited by 7 publications

References 30 publications

Sample preparation and fractionation techniques for intact proteins for mass spectrometric analysis

Sample preparation and fractionation techniques for intact proteins for mass spectrometric analysis

A decade of microchip electrophoresis for clinical diagnostics – A review of 2008–2017

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

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