Simplified Preliminary Blood Testing. An Improved Technique and a Comparative Study of Methods

Grodsky, Morris; Wright, Keith; Kirk, Paul L.

doi:10.2307/1140307

Cited by 61 publications

(46 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These facts are noted here, not because any remedy for the variability has been found, but because the difficulty was not fully appreciated in earlier work (1). Failure of other workers to find as satisfactory results as claimed can result in distrust of the basic method, when actually the answer will undoubtedly be found in some variation of the method of performing the test.…”

Section: The Puenemorpnha= Testmentioning

confidence: 75%

See 1 more Smart Citation

Some Problems in Blood Testing and Grouping

Kirk¹,

Brown²,

Conners³

1954

The Journal of Criminal Law, Criminology, and Police Science

Self Cite

View full text Add to dashboard Cite

Section: The Puenemorpnha= Testmentioning

confidence: 75%

“…Several difficulties in routine application of blood testing methods (1), and particularly of blood grouping (2) have arisen in this and other laboratories. The reasons for some of these and methods of avoiding or correcting the difficulties need to be called to the attention of workers in crime laboratories.…”

mentioning

confidence: 99%

Some Problems in Blood Testing and Grouping

Kirk¹,

Brown²,

Conners³

1954

The Journal of Criminal Law, Criminology, and Police Science

Self Cite

View full text Add to dashboard Cite

“…Para a preparação de luminol primeiramente foi dissolvido 0,07 g de perborato de sódio em 10 mL de água, na sequência foi adicionado 0,01g de luminol e 0,5 g de carbonato de sódio [20].…”

Section: Preparação Do Reagente Luminolunclassified

A interferência da solução de luminol em teste imunocromatográfico para pesquisa de sangue humano

Vaz¹,

Kobachuck²

2017

Rev. Bras. Crimin.

View full text Add to dashboard Cite

ResumoAs manchas de sangue muitas vezes são limpas nas cenas de crime, e um dos métodos para identificação de sangue latente é o luminol, o qual possui alta sensibilidade, porém, pouca especificidade, e por esse motivo, deve ser acompanhado de um método de confirmação. Este estudo teve como objetivo analisar a possível interferência do teste presuntivo luminol, preparado de acordo com a formulação de Grodsky sobre o teste confirmatório imunocromatográfico (WAMA®) para pesquisa de sangue humano e verificar a sensibilidade de ambos os testes. Diferentes diluições de uma amostra de sangue humano foram depositadas sobre fragmentos de tecido de algodão branco, deixadas secar por 24 horas em temperatura ambiente e, em seguida, foram submetidas aos dois métodos. Após as amostras terem sido pulverizadas com luminol, a diluição 1:1.000 não mostrou interferência no teste feito imediatamen te após a pulverização, porém após 24 horas a interferência foi observada. Na diluição 1:10.000 houve interferência imediata no teste imunocromatográfico e o resultado persistiu após 24 horas. Já as diluições de 1: 50.000 e 1:100.000 tanto o controle positivo quanto a amostra pulverizada por luminol apresentaram resultados negativos imediatamente e após 24 horas no teste imunocromatográfico. Resultado positivo para o teste de luminol com emissão de quimiluminescência foi obtido até a concentração 1:50.000 e o teste imunocromatográfico mostrou-se positivo até a diluição 1:10.000, indicando que realmente há uma diferença de sensibilidade entre os métodos.Palavras-Chave: Crime; Sangue; Luminol; Imunocromatografia. AbstractBlood stains are often cleaned the crime scene, and one of the methods for latent blood identification is luminol, which has high sensitivity, but low specificity and therefore must be accompanied by a confirmatory method. This study aimed to analyze the possible interference of the presumptive luminol test, prepared according to Grodsky formulation of the immunoassay confirmatory test (WAMA®) for human blood research and check the sensitivity of both tests. Different dilutions of a human blood sample we re deposited on white cotton fabric fragments, allowed to dry for 24 hours at room temperature and then were subjected to two methods. After the samples have been sprayed with luminol, dilution 1: 1000 showed no interference with the test done immediately afte r the spray, but after 24 hours was observed interference. In the 1: 10,000 dilution was no immediate interference in the immunoassay test and the results persisted after 24 hours. Since dilutions of 1: 50,000 and 1: 100,000 both as positive control sample by spraying luminol showed negative results immediately and after 24 hours in the immunoassay. Positive for the luminol test with chemiluminescence emission was obtained by the concentration of 1:50,000 and the immunoassay was positive up to 1:10,000 dilution, indicating that there really is difference in sensitivity between the methods.

show abstract

“…that has been placed in a saline solution or water (Grodsky et al 1951). A small drop of the extracted sample is then placed into a solution containing phenolphthalein ( Fig.…”

Section: Blood Evidencementioning

confidence: 99%

“…1). Blood from all animal species has different proteins therein; thus, blood from one species will not be accepted by another species based upon the differences in the interacting proteins within the two different blood types (Grodsky et al 1951). If specific blood type must be determined, an ABO test can be performed on the sample (Fig.…”

Section: Blood Evidencementioning

confidence: 99%

Forensic blood evidence analysis using RNA targets and novel molecular tools

Counsil

McKillip

2010

Biologia

View full text Add to dashboard Cite

In the realm of forensic investigation, messenger RNA (mRNA) is a good source for specimen analysis, owing to tissue-specific expression patterns for many transcripts. Thus, differentiation of bodily fluids collected at a crime scene is possible if the proper target mRNA is identifiable and detectable using molecular methods. With the analysis of menstrual blood evidence, for example, the matrix metalloproteinase (MMP) family of genes encodes proteins involved in extracellular matrix breakdown during tissue remodeling, and in particular MMP11 and MMP7 mRNA have proven useful for forensic blood evidence analysis. Nucleic acid sequence based amplification (NASBA) is a highly sensitive RNA transcription-based amplification method used to detect gene expression. As an emerging real-time technology, NASBA holds promise as a tool to rapidly and sensitively amplify mRNA markers associated with the MMP genes to verify the presence of menstrual blood in a forensic sample, and has a number of advantages compared to reverse transcriptase polymerase chain reaction. The purpose of this review is to overview the current state of mRNA analysis in forensic evidence casework and to highlight areas of research, progress, and development in the near-term future that will lead to reduction of case evidence backlog in crime laboratories in developed countries.

show abstract

Simplified Preliminary Blood Testing. An Improved Technique and a Comparative Study of Methods

Cited by 61 publications

References 6 publications

Some Problems in Blood Testing and Grouping

Some Problems in Blood Testing and Grouping

A interferência da solução de luminol em teste imunocromatográfico para pesquisa de sangue humano

Forensic blood evidence analysis using RNA targets and novel molecular tools

Contact Info

Product

Resources

About